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Alberta NINT/5 August 2008
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[[Team:Alberta_NINT/Notebook | < Back to notebook]] | [[Team:Alberta_NINT/Notebook | < Back to notebook]] | ||
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lab work (JD): | lab work (JD): | ||
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Incubated at 37C | Incubated at 37C | ||
Annealed new gates TA2, TA3, TA6 and TA7 | Annealed new gates TA2, TA3, TA6 and TA7 | ||
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Latest revision as of 23:06, 11 October 2008
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lab work (JD):
Collected some quantitative data of K102011/X+H and TAIn_X/X+H
Found that perhaps a 1/100 dilution of insert might work better for ligation
Carried out ligation of K102011 vector with TAIn_X inserts
Transformed XL1-B Cells
Plated on LB+kan35
Incubated at 37C
Annealed new gates TA2, TA3, TA6 and TA7
