Wisconsin: Lignin Project/24 June 2008

Attempted to isolate genomic DNA from E. coli MG1655 using the Weibel lab protocols.
Using this isolated DNA ran a pcr reaction as before and got one successful band at 700bp.
We purified and stored this band at -20.
After discussions with faculty new C-Media recipe was obtained.

Team Fungus:
Ran leftovers from yesterday's PCR in 1% agrose gel
-correct sized bands visible in UV light
Used QIAquick gel extraction microcentrifuge kit and QIAquick PCR purification to get purified product
Used QIAprep miniprep kit to purify pET28a vectors from liquid LB + Kanamycin media that had incubated overnight
Ran another touchdown PCR of both Carbon and Nitrogen limited cDNA using Herculase II polymerase
Used QIAquick PCR purification kit to purify PCR product