"
Wisconsin: Lignin Project/30 June 2008
From 2008.igem.org
| (3 intermediate revisions not shown) | |||
| Line 6: | Line 6: | ||
|'''Team Sorbitol:''' | |'''Team Sorbitol:''' | ||
| - | Repeated the successful PCR reaction from before. | + | Repeated the successful PCR reaction from before.<br> |
| - | + | This time we obtained successful band for both ''srlD'' 700bp and the ''srl'' operon 2700bp<br> | |
| - | This time we obtained successful band for both ''srlD'' 700bp and the ''srl'' operon 2700bp | + | These products were purified and stored at 4C overnight.<br> |
| - | + | Some of the product was used to start a second PCR reaction to run overnight.<br> | |
| - | These products were purified and stored at 4C overnight. | + | Gowth curves using revised Cmedia with additional components completed to determine optimal growth media.<br> |
| - | + | <br> | |
| - | Some of the product was used to start a second PCR reaction to run overnight. | + | '''Team Fungus:''' <br> |
| + | Grew BL21 pET28a plates for colonies 1 & 2, streaked out new plates, and selected colonies and grew them in liquid media over the weekend.<br> | ||
| + | Minipreped to purify DNA from colonies and stored them on ice.<br> | ||
| + | Ran a second PCR to test results and get more colonies, however electrophoresis returned inconclusive results.<br> | ||
| + | Digested purified plasmid and ran out on 1% agarose gel. Colonies 1a and 1b possibly have insert.<br> | ||
| + | Ran PCR using previous PCR products as template. Ran out on 1% agarose gel. Saw faint bands. <br> | ||
| + | Ran a PCR using purified plasmids as templates.<br> | ||
| + | Made LB broth.<br> | ||
|} | |} | ||
Latest revision as of 02:02, 29 October 2008
| Team Sorbitol:
Repeated the successful PCR reaction from before. |
