Wisconsin: Lignin Project/2 July 2008

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(Difference between revisions)

Latest revision as of 02:32, 29 October 2008

Team Sorbitol:

The pBAD30 was harvested using a Qiagen Miniprep kit and digested as described before.
We were able to extract enough plasmid DNA to perform a ligation.
We then set up a ligation with the following rations of vector to insert: 5:1, 3:1, 1:1, 1:3, 1:5, 1:0, 0:1
The mixture was allowed to ligate at 4C overnight.
Team Fungus:
Ran out PCR from last night on 1% agarose gel. Correct product size.
Lab meeting.
Made overnight cultures of cells containing pET28a plasmid.
Diluted successful PCR product and used as template in another PCR.
Purified PCR product. Nanodrop giving inconsistent readings, but mostly too low.

@@ Line 10: / Line 10: @@
 We then set up a ligation with the following rations of vector to insert: 5:1, 3:1, 1:1, 1:3, 1:5, 1:0, 0:1<br>
 The mixture was allowed to ligate at 4C overnight.<br>
+'''Team Fungus:'''<br>
+Ran out PCR from last night on 1% agarose gel. Correct product size.<br>
+Lab meeting.<br>
+Made overnight cultures of cells containing pET28a plasmid.<br>
+Diluted successful PCR product and used as template in another PCR.<br>
+Purified PCR product. Nanodrop giving inconsistent readings, but mostly too low.<br>
 |}