Wisconsin: Lignin Project/3 July 2008

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To do this we followed the protocol of the Weibel Lab.<br>
To do this we followed the protocol of the Weibel Lab.<br>
We plated the transformations on LB/Amp (100ug/mL) overnight at 30C<br>
We plated the transformations on LB/Amp (100ug/mL) overnight at 30C<br>
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further growth curves completed with C-media and TpiA knockout strain JW3890.<br>
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'''Team Fungus:'''<br>
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Purified pET28a from overnight culture.<br>
 +
Ran out yesterday's 2nd PCR product on 1% agarose gel. Correct band size.<br>
 +
Purified PCR product from yesterday's 2nd PCR.<br>
 +
Digested both purified PCR product and pET28a with EcoRI and XhoI.<br>
 +
Ran digestion products out on gel. pET28a product too big?<br>
 +
Grew another overnight of pET28a.<br>
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Latest revision as of 02:40, 29 October 2008

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Team Sorbitol:

Created SOC media for transformations.
Transformed each of the ligations (as well as controls) into our chemically competent stocks of DH5a.
To do this we followed the protocol of the Weibel Lab.
We plated the transformations on LB/Amp (100ug/mL) overnight at 30C
further growth curves completed with C-media and TpiA knockout strain JW3890.
Team Fungus:
Purified pET28a from overnight culture.
Ran out yesterday's 2nd PCR product on 1% agarose gel. Correct band size.
Purified PCR product from yesterday's 2nd PCR.
Digested both purified PCR product and pET28a with EcoRI and XhoI.
Ran digestion products out on gel. pET28a product too big?
Grew another overnight of pET28a.

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