Wisconsin:Lignin Project/29 August 2008

From 2008.igem.org

(Difference between revisions)
(New page: <div style="background: #000; padding: 10px;"> <div style="width:800px; height:147px; border:0px; margin:0px;">Image:Igemwibanner.gif</div> {|align="justify" style="color:#aada84;bac...)
 
(3 intermediate revisions not shown)
Line 4: Line 4:
{|align="justify" style="color:#aada84;background-color:#000;" width="800 px"
{|align="justify" style="color:#aada84;background-color:#000;" width="800 px"
 +
|'''Team Sorbitol:'''<br>
 +
Performed PCR cleanup and reactions for sequencing.<br>
 +
Submitted for sequencing.<br>
 +
Sequencing results received same day. All colonies perfect matches with new primers. Success!<br>
 +
Ligation product transformed into E.coli.<br>
 +
Second His-tag purification resulted again with no protein.  Now it is believed to be too small an amount for the nanodrop to measure.
 +
 +
'''Team Fungus:'''<br>
 +
Remade assay solutions due to contaminants.<br>
 +
Re-ran Azure B assay on 96 well plate.<br>
|}
|}

Latest revision as of 02:45, 29 October 2008

Igemwibanner.gif
Team Sorbitol:

Performed PCR cleanup and reactions for sequencing.
Submitted for sequencing.
Sequencing results received same day. All colonies perfect matches with new primers. Success!
Ligation product transformed into E.coli.
Second His-tag purification resulted again with no protein. Now it is believed to be too small an amount for the nanodrop to measure.

Team Fungus:
Remade assay solutions due to contaminants.
Re-ran Azure B assay on 96 well plate.

Retrieved from "http://2008.igem.org/Wisconsin:Lignin_Project/29_August_2008"