"
User:University of Washington/8 August 2008
From 2008.igem.org
(Difference between revisions)
(→MG1655Z1) |
|||
| (2 intermediate revisions not shown) | |||
| Line 1: | Line 1: | ||
| - | == pLux Regulation == | + | == pLux Regulation (Scott)== |
PCR of TrbA and KorA for making BioBrick parts<br> | PCR of TrbA and KorA for making BioBrick parts<br> | ||
| Line 6: | Line 6: | ||
3.KorA - pUB307<br> | 3.KorA - pUB307<br> | ||
4.KorA - RP4 | 4.KorA - RP4 | ||
| + | |||
| + | ==LuxR from AraC and TetR(Faifan)== | ||
| + | |||
| + | -QuikChange trial#3 didn't seem to work. There was no growth in the reaction 1 and 2. There was no growth in negative(no primers) and growth in positive(no Dpn1) which said Dpn1 worked. | ||
| + | *Ingrid set up new reactions(trial#4). | ||
| + | *Tried tranformation again with 5 ul and 15 ul of DNA. (Last time used 2 ul), plated onto Amp. | ||
| + | |||
| + | -Miniprepped and sent sequencing for potential new BioBrick part: promoter AraC and TetR (from Elowitz) on pSB1AC3. | ||
| + | |||
| + | ==MG1655Z1(Faifan)== | ||
| + | |||
| + | -Got lawn of E.coli on Tsy plates. The culture wasn't diluted enough(used 1:100 twice, supposed to be 1:1000 twice). | ||
| + | |||
| + | -Streaked out cultures from the plate with lawn of cells into new Tsy plates. | ||
| + | |||
---- | ---- | ||
Back to [[Team:University_of_Washington/Notebook#Notebook]] | Back to [[Team:University_of_Washington/Notebook#Notebook]] | ||
Latest revision as of 22:56, 8 August 2008
pLux Regulation (Scott)
PCR of TrbA and KorA for making BioBrick parts
1.TrbA - pUB307
2.TrbA - RP4
3.KorA - pUB307
4.KorA - RP4
LuxR from AraC and TetR(Faifan)
-QuikChange trial#3 didn't seem to work. There was no growth in the reaction 1 and 2. There was no growth in negative(no primers) and growth in positive(no Dpn1) which said Dpn1 worked.
- Ingrid set up new reactions(trial#4).
- Tried tranformation again with 5 ul and 15 ul of DNA. (Last time used 2 ul), plated onto Amp.
-Miniprepped and sent sequencing for potential new BioBrick part: promoter AraC and TetR (from Elowitz) on pSB1AC3.
MG1655Z1(Faifan)
-Got lawn of E.coli on Tsy plates. The culture wasn't diluted enough(used 1:100 twice, supposed to be 1:1000 twice).
-Streaked out cultures from the plate with lawn of cells into new Tsy plates.
Back to Team:University_of_Washington/Notebook#Notebook
