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User:University of Washington/8 August 2008
From 2008.igem.org
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-Miniprepped and sent sequencing for potential new BioBrick part: promoter AraC and TetR (from Elowitz) on pSB1AC3. | -Miniprepped and sent sequencing for potential new BioBrick part: promoter AraC and TetR (from Elowitz) on pSB1AC3. | ||
| - | ==MG1655Z1== | + | ==MG1655Z1(Faifan)== |
-Got lawn of E.coli on Tsy plates. The culture wasn't diluted enough(used 1:100 twice, supposed to be 1:1000 twice). | -Got lawn of E.coli on Tsy plates. The culture wasn't diluted enough(used 1:100 twice, supposed to be 1:1000 twice). | ||
Latest revision as of 22:56, 8 August 2008
pLux Regulation (Scott)
PCR of TrbA and KorA for making BioBrick parts
1.TrbA - pUB307
2.TrbA - RP4
3.KorA - pUB307
4.KorA - RP4
LuxR from AraC and TetR(Faifan)
-QuikChange trial#3 didn't seem to work. There was no growth in the reaction 1 and 2. There was no growth in negative(no primers) and growth in positive(no Dpn1) which said Dpn1 worked.
- Ingrid set up new reactions(trial#4).
- Tried tranformation again with 5 ul and 15 ul of DNA. (Last time used 2 ul), plated onto Amp.
-Miniprepped and sent sequencing for potential new BioBrick part: promoter AraC and TetR (from Elowitz) on pSB1AC3.
MG1655Z1(Faifan)
-Got lawn of E.coli on Tsy plates. The culture wasn't diluted enough(used 1:100 twice, supposed to be 1:1000 twice).
-Streaked out cultures from the plate with lawn of cells into new Tsy plates.
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