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Team:The University of Alberta/11 August 2008
From 2008.igem.org
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(New page: ==Today== '''Chris''' *Got the sequence results back from Friday: Looks like the suspicious band was Tryp after all. Will go ahead with the ligation into Tryp. Ive decided that if it doesn...) |
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'''Chris''' | '''Chris''' | ||
*Got the sequence results back from Friday: Looks like the suspicious band was Tryp after all. Will go ahead with the ligation into Tryp. Ive decided that if it doesnt work this third time then Tryp+Blue Ox is a lost cause. It would probably be a better use of our time to get RFP ino pTetR while mutating Purple Russian than to keep spending inordinate amounts of time on Blue Ox + Tryp. | *Got the sequence results back from Friday: Looks like the suspicious band was Tryp after all. Will go ahead with the ligation into Tryp. Ive decided that if it doesnt work this third time then Tryp+Blue Ox is a lost cause. It would probably be a better use of our time to get RFP ino pTetR while mutating Purple Russian than to keep spending inordinate amounts of time on Blue Ox + Tryp. | ||
| + | **Digested the Tryp PCR product with Xba and Pst so I can ligate it into J6 cut with Xba and Pst (digested previously) | ||
| + | **Gel purified digest products - resulted in low concentration (9.1ug/mL) but will have to do | ||
| + | '''Saima''' | ||
| + | *Since the transformation of Cam35s+J6 ligations didn't work, we question the efficacy of our Ligase. To check that, we took J61003 digested with Xba1 and ligated it. If the tranformations don't work, we will conclude our ligase is not working. Results to be back tomorrow. | ||
| + | *Set up a digestion of Cam35s (PCRd and gel purified) with Spe/Pst. | ||
| + | *Set up a digestion of Lac1QERE with Xba1/Pst. | ||
Latest revision as of 16:39, 12 August 2008
Today
Chris
- Got the sequence results back from Friday: Looks like the suspicious band was Tryp after all. Will go ahead with the ligation into Tryp. Ive decided that if it doesnt work this third time then Tryp+Blue Ox is a lost cause. It would probably be a better use of our time to get RFP ino pTetR while mutating Purple Russian than to keep spending inordinate amounts of time on Blue Ox + Tryp.
- Digested the Tryp PCR product with Xba and Pst so I can ligate it into J6 cut with Xba and Pst (digested previously)
- Gel purified digest products - resulted in low concentration (9.1ug/mL) but will have to do
Saima
- Since the transformation of Cam35s+J6 ligations didn't work, we question the efficacy of our Ligase. To check that, we took J61003 digested with Xba1 and ligated it. If the tranformations don't work, we will conclude our ligase is not working. Results to be back tomorrow.
- Set up a digestion of Cam35s (PCRd and gel purified) with Spe/Pst.
- Set up a digestion of Lac1QERE with Xba1/Pst.
