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Wisconsin: Lignin Project/11 July 2008
From 2008.igem.org
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created chemically competent stocks of MG1655, JW3890 and RL357.<br> | created chemically competent stocks of MG1655, JW3890 and RL357.<br> | ||
The stocks were frozen down and kept at -80C for future use.<br> | The stocks were frozen down and kept at -80C for future use.<br> | ||
| + | changing growth temperature increased growth of tpiA knockout greatly.<br> | ||
'''Team Fungus'''<br> | '''Team Fungus'''<br> | ||
Researched the necessary environment for the disulfide bond formation needed in correct folding of LiP <br> | Researched the necessary environment for the disulfide bond formation needed in correct folding of LiP <br> | ||
| - | Concluded that a oxidative cytoplasm was needed, thus the Novagen Origami strains were chosen as final transformation hosts. | + | Concluded that a oxidative cytoplasm was needed, thus the Novagen Origami strains were chosen as final transformation hosts, specifically Rosetta-gami2(DE3).<br> |
| + | Ran out gels of all tubes in freezer. Found five tubes with correct size bands.<br> | ||
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Latest revision as of 03:30, 29 October 2008
| Team Sorbitol Froze down 3 400ul samples of the overnight DH5a-pBAD30-Srld E.coli for future use. |
