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Wisconsin:Lignin Project/7 August 2008
From 2008.igem.org
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| - | |'''Team Fungus:'''<br> | + | |'''Team Sorbitol:'''<br> |
| + | Set up a Digestion of the srlD PCR product as well as the pET28a vector. The Products were verified on a gel and extracted using a gel purification kit.<br> | ||
| + | Performed practice assay on sheep liver sorbitol dehydrogenase. A statistically significant amount of replicants were done for eventual E.coli SDH comparison assays. | ||
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| + | '''Team Fungus:'''<br> | ||
Transformed into Rosetta-gami 2(DE3)pLysS.<br> | Transformed into Rosetta-gami 2(DE3)pLysS.<br> | ||
Made overnight cultures of colonies 5 & 6 in LB + KAN (30 ug/mL) media. <br> | Made overnight cultures of colonies 5 & 6 in LB + KAN (30 ug/mL) media. <br> | ||
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Latest revision as of 00:20, 29 October 2008
| Team Sorbitol: Set up a Digestion of the srlD PCR product as well as the pET28a vector. The Products were verified on a gel and extracted using a gel purification kit. Team Fungus: |
