Wisconsin: Lignin Project/31 July 2008

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{|align="justify" style="color:#aada84;background-color:#000;" width="800 px"
{|align="justify" style="color:#aada84;background-color:#000;" width="800 px"
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|'''Team Fungus:'''<br>
+
|'''Team Sorbitol:'''<br>
 +
Competent cells for MG1655, pBAD30,pBAD18, and DH5a<br>
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<br>
 +
'''Team Fungus:'''<br>
Ran digestion of pET28a and ran out on .75% agrose gel.  All bands were correct size. <br>
Ran digestion of pET28a and ran out on .75% agrose gel.  All bands were correct size. <br>
Used the QIAquick gel extraction kit to purify the double digested vector band.  Spec reading of 14 ng/uL.<br>
Used the QIAquick gel extraction kit to purify the double digested vector band.  Spec reading of 14 ng/uL.<br>
Ran overnight ligation at 4 degrees C.
Ran overnight ligation at 4 degrees C.
|}
|}

Latest revision as of 03:11, 29 October 2008

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Team Sorbitol:

Competent cells for MG1655, pBAD30,pBAD18, and DH5a

Team Fungus:
Ran digestion of pET28a and ran out on .75% agrose gel. All bands were correct size.
Used the QIAquick gel extraction kit to purify the double digested vector band. Spec reading of 14 ng/uL.
Ran overnight ligation at 4 degrees C.