Wisconsin: Lignin Project/31 July 2008
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{|align="justify" style="color:#aada84;background-color:#000;" width="800 px" | {|align="justify" style="color:#aada84;background-color:#000;" width="800 px" | ||
- | |'''Team Fungus:'''<br> | + | |'''Team Sorbitol:'''<br> |
+ | Competent cells for MG1655, pBAD30,pBAD18, and DH5a<br> | ||
+ | <br> | ||
+ | '''Team Fungus:'''<br> | ||
Ran digestion of pET28a and ran out on .75% agrose gel. All bands were correct size. <br> | Ran digestion of pET28a and ran out on .75% agrose gel. All bands were correct size. <br> | ||
Used the QIAquick gel extraction kit to purify the double digested vector band. Spec reading of 14 ng/uL.<br> | Used the QIAquick gel extraction kit to purify the double digested vector band. Spec reading of 14 ng/uL.<br> | ||
Ran overnight ligation at 4 degrees C. | Ran overnight ligation at 4 degrees C. | ||
|} | |} |
Latest revision as of 03:11, 29 October 2008
Team Sorbitol: Competent cells for MG1655, pBAD30,pBAD18, and DH5a |