EPF-Lausanne/12 August 2008

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(→Molecular Biology, assemblage of biobricks)
(→F1610 and E1010)
 
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=Molecular Biology, assemblage of biobricks=
=Molecular Biology, assemblage of biobricks=
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All cells culture work but unfortunately, there is a problem in mini-prep procedure, any one have more than 30 ng/ul . So we do again new cell cultures.
+
All cells culture worked but unfortunately, there seems to have been a problem in the mini-prep procedure, as none of them have more than 30 ng/ul of DNA. So we redo new cell cultures once more.
   
   
==F1610 and E1010==
==F1610 and E1010==
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We do a gel to see if we can see fragments(we cut plasmids with SpeI and EcoRI to see size of insert). Unfortunately we see nothings, any fragment with 1500 bp. But the positive control doesn't work too. The positive control is F1610(800 bp) and E1010 (700 bp). So we decide to do tomorrow a test of digestion with only E1010 and F1610 that we use for positive control compare to a new digestion of these Biobricks that we do today. To see if there is problem in digestion procedure.
+
We do a gel to check if the ligation has worked (we cut the plasmids with SpeI and EcoRI to see the size of the insert). Unfortunately we don't see any fragment around the expected 1500 bp. However the positive control doesn't work either. The positive controls are F1610 (800 bp) and E1010 (700 bp).  
 +
We suspect an error in the digestion process, so we decide to do a new digestion tommorrow, using both the E1010 and F1610 that we used for positive control and new digestions of the same parts.
==R0040/B0034 and R0071/B0034==
==R0040/B0034 and R0071/B0034==
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We do transformation
+
We do the transformation.

Latest revision as of 09:19, 19 August 2008

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Molecular Biology, assemblage of biobricks

All cells culture worked but unfortunately, there seems to have been a problem in the mini-prep procedure, as none of them have more than 30 ng/ul of DNA. So we redo new cell cultures once more.

F1610 and E1010

We do a gel to check if the ligation has worked (we cut the plasmids with SpeI and EcoRI to see the size of the insert). Unfortunately we don't see any fragment around the expected 1500 bp. However the positive control doesn't work either. The positive controls are F1610 (800 bp) and E1010 (700 bp). We suspect an error in the digestion process, so we decide to do a new digestion tommorrow, using both the E1010 and F1610 that we used for positive control and new digestions of the same parts.

R0040/B0034 and R0071/B0034

We do the transformation.

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