Imperial College/17 August 2008
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|{{#calendar: title=Imperial_College |year=2008 | month=08}} | |{{#calendar: title=Imperial_College |year=2008 | month=08}} | ||
|{{#calendar: title=Imperial_College |year=2008 | month=09}} | |{{#calendar: title=Imperial_College |year=2008 | month=09}} | ||
- | | rowspan="2" bgcolor=# | + | | rowspan="2" bgcolor=#ffffff width="100%" | |
|} | |} | ||
- | = | + | =17 August 2008= |
==Wet Lab== | ==Wet Lab== | ||
===''B.subtilis=== | ===''B.subtilis=== | ||
+ | =====Monday===== | ||
+ | *Prepare media and reagents for the protocol transformation 1 protocol | ||
+ | *Prepare 2x10ml LB media in 100ml flasks | ||
+ | *Prepare 2x100ml LB media in 1000ml flasks | ||
+ | *Prepare 2x overnight cultures in the 10ml LB media flasks | ||
+ | *Prepare a 1% agarose gel and run the digest prepared on friday | ||
+ | |||
+ | =====Tuesday===== | ||
+ | *Prepare competent cells using the transformation 2 protocol, this time growing cells to an o.D.<sub>600</sub> | ||
+ | *Prepare 1x20ml of LB media in a 200ml flask (autoclaved in the morning) | ||
+ | *Prepare 1x20ml of SpC media in a 200ml flask (autoclaved in the morning) | ||
+ | *Prepare aliquots of SpII media (autoclaved in the morning) | ||
+ | |||
+ | =====Wednesday===== | ||
+ | *Prepare competent cells using the transformation 1 protocol, | ||
+ | *Electroporate the compete cells from the transformation protocol 2 using a range of concentrations | ||
+ | |||
+ | =====Thursday===== | ||
+ | *Transform the competent cells prepared from transformation 2 protocol | ||
+ | *Check the transformation cells from protocol 2, if transformed correctly then carry the test for correct integration, | ||
+ | |||
+ | =====Friday===== | ||
+ | *If the transformation has been successful then carry out the integration test. | ||
===Cloning=== | ===Cloning=== | ||
+ | |||
=====Monday===== | =====Monday===== | ||
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*PCR check the minipreps that could be digested by ''XbaI'' and ''SpeI'' | *PCR check the minipreps that could be digested by ''XbaI'' and ''SpeI'' | ||
+ | <br> | ||
+ | {{Imperial/EndPage|Notebook|Notebook}} |
Latest revision as of 20:37, 28 October 2008
17 August 2008Wet LabB.subtilisMonday
Tuesday
Wednesday
Thursday
Friday
CloningMonday
Tuesday
Wednesday
Thursday
Friday
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