Team:NTU-Singapore/Parts/Fluorescence Microscope Image

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=Image Results obtained from fluorescence microscope=
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='''Images and Videos from the Characterization Lab'''=
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Below are Images obtained from the microscope on the various standards that iGEM MIT has provided for us this year.
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=='''pLacI-GFP part:BBa_J04430 found to express RFP instead'''==
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==Standard 102==
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The below pictures were taken after the BBa_J04430 was transformed into Top 10 competent cells. They clearly showed, either under normal light or UV light, the cells appears red or glow red respectively. This is contrary to what was expected, as we would expect it to glow green under UV light. This problem was highlighted to the iGEM Headquarters. In order to solve this problem the pLac promoter (R0010) was ligated with the GFP (E0040), allowing us to proceed further with the characterization experiments.
{| align="center" border="1"
{| align="center" border="1"
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|[[Image:102-norm_030708.JPG|thumb|400px|102 Normal]]
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|[[Image:NTU@iGEM_Pink_gfp.jpg|thumb|400px|Cells appears pinkish even under normal light]]
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|[[Image:102_gfp_030708.jpg|400px|GFP]]
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|[[Image:NTU@iGEM_(UV pink_gfp).JPG|thumb|400px|Cells glow PINK under UV light]]
|}
|}
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==Standard 150==
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=='''Image results obtained from fluorescence microscope'''==
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Below are Images obtained from the microscope of the various standards (Low, Medium, High & Standard) that iGEM Headquarters has provided for us in the [http://partsregistry.org/Measurement/SPU/Learn iGEM Newsletter].
 +
 
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===Weak promoter [http://partsregistry.org/Part:BBa_J23150 BBa_J23150]===
{| align="center" border="1"
{| align="center" border="1"
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|[[Image: 150_norm_030708.JPG|400px|Normal]]
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|[[Image:102-norm_030708.JPG|thumb|400px|BBa_J23150 Before Excitation]]
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|[[Image: 150_gfp_030708.JPG|400px|GFP]]
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|[[Image:NTU@iGEM_Weak_GREEN_gfp_030708.jpg|thumb|400px|BBa_J23150 After Excitation]]
|}
|}
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==Standard 151==
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===Medium promoter [http://partsregistry.org/Part:BBa_J23151 BBa_J23151]===
{| align="center" border="1"
{| align="center" border="1"
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|[[Image: 151_norm_030708.JPG|400px|Normal]]
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|[[Image: 150_norm_030708.JPG|thumb|400px|BBa_J23151 Before Excitation ]]
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|[[Image: 151_gfp_030708.JPG|400px|GFP]]
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|[[Image: NTU@iGEM_Medium_GREEN_gfp_030708.jpg|thumb|400px|BBa_J23151 After Excitation ]]
|}
|}
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==Standard 260==
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===Strong promoter [http://partsregistry.org/Part:BBa_J23102 BBa_J23102] ===
{| align="center" border="1"
{| align="center" border="1"
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|[[Image:260norm_030708.JPG|400px|Normal]]
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|[[Image: 151_norm_030708.JPG|thumb|400px|BBa_J23102 Before Excitation]]
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|[[Image:260gfp_030708_b.JPG|400px|GFP]]
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|[[Image: NTU@iGEM_Strong_GREEN_gfp_030708.jpg|thumb|400px|BBa_J23102 After Excitation]]
|}
|}
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The above picture shows the green fluorescence produced by Top 10 ecoli cells under blue light excitation. These Ecoli cells contain the standard promoter 260 with GFP and thye have been allowed to grow to an OD of 1.2 before a snap shot is taken.
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===Standard promoter with GFP reporter [http://partsregistry.org/Part:BBa_I20260 BBa_I20260]  ===
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{| align="center" border="1"
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|[[Image:260norm_030708.JPG|thumb|400px|BBa_I20260Before Excita]]
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|[[Image:NTU@iGEM_Standard_GREEN_gfp_030708.jpg|thumb|400px|BBa_I20260After Excitation]]
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|}
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The above pictures showed the green fluorescence protein was produced by Top 10 competent cells that were taken under blue light excitation. These cells contained the different standard promoters with GFP and they have been allowed to grow to an OD of 1.2 before a snap shot was taken with the microscope.
=Video obtained from fluorescence microscope=
=Video obtained from fluorescence microscope=
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Video of E coli under blue light excitation
Video of E coli under blue light excitation
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Latest revision as of 05:50, 27 October 2008

Contents

Images and Videos from the Characterization Lab

pLacI-GFP part:BBa_J04430 found to express RFP instead

The below pictures were taken after the BBa_J04430 was transformed into Top 10 competent cells. They clearly showed, either under normal light or UV light, the cells appears red or glow red respectively. This is contrary to what was expected, as we would expect it to glow green under UV light. This problem was highlighted to the iGEM Headquarters. In order to solve this problem the pLac promoter (R0010) was ligated with the GFP (E0040), allowing us to proceed further with the characterization experiments.

Cells appears pinkish even under normal light
Cells glow PINK under UV light

Image results obtained from fluorescence microscope

Below are Images obtained from the microscope of the various standards (Low, Medium, High & Standard) that iGEM Headquarters has provided for us in the [http://partsregistry.org/Measurement/SPU/Learn iGEM Newsletter].

Weak promoter [http://partsregistry.org/Part:BBa_J23150 BBa_J23150]

BBa_J23150 Before Excitation
BBa_J23150 After Excitation

Medium promoter [http://partsregistry.org/Part:BBa_J23151 BBa_J23151]

BBa_J23151 Before Excitation
BBa_J23151 After Excitation

Strong promoter [http://partsregistry.org/Part:BBa_J23102 BBa_J23102]

BBa_J23102 Before Excitation
BBa_J23102 After Excitation

Standard promoter with GFP reporter [http://partsregistry.org/Part:BBa_I20260 BBa_I20260]

BBa_I20260Before Excita
BBa_I20260After Excitation

The above pictures showed the green fluorescence protein was produced by Top 10 competent cells that were taken under blue light excitation. These cells contained the different standard promoters with GFP and they have been allowed to grow to an OD of 1.2 before a snap shot was taken with the microscope.

Video obtained from fluorescence microscope


Video of E coli under normal white light



Video of E coli under blue light excitation

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