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Team:BCCS-Bristol/Protocols-DMSO stocks
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# Store the DMSO culture in the -80°C freezer | # Store the DMSO culture in the -80°C freezer | ||
# In the evening (or in one of the following days), make a streak out of the stock to see whether the bacteria are ok | # In the evening (or in one of the following days), make a streak out of the stock to see whether the bacteria are ok | ||
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Latest revision as of 09:47, 14 September 2008
DMSO stocks
- Make an overnight culture of the bacteria in LB broth (If the strain carries a resistance gene on a plasmid, you have to use LB with antibiotics)
- Next morning, put 70 µl DMSO into a cryotube that you labelled with the strain’s name and the date
- Add 930 µl overnight culture
- Incubate immediately for 30 min on ice
- Store the DMSO culture in the -80°C freezer
- In the evening (or in one of the following days), make a streak out of the stock to see whether the bacteria are ok
