EPF-Lausanne/19 August 2008

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Latest revision as of 16:20, 19 August 2008

PCR of the small parts

As the result on the last PCR ("checking all the parts") was unclear (or even negative) for several small parts, we do the PCR once more to check the results.

Result: The parts are clearly visible this time. Only thing is that there are bands visible at around 5kbp. Therefore we will redo a gel (with more spacing) for a gel purification and subsequent PCR. This way we should have only the desired parts left.

PCR of LacIM from PHD1, PHD2 and PHD3

Using the primers designed by Alex with the Biobricks prefix and suffix, we maid a PCR of the PHD1, PHD2 and PHD3 plasmids to amplify the LacIM gene and have it as a Biobrick available for our construct.

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+==PCR of the small parts==
+As the result on the last PCR ("checking all the parts") was unclear (or even negative) for several small parts, we do the PCR once more to check the results.
+Result: The parts are clearly visible this time. Only thing is that there are bands visible at around 5kbp. Therefore we will redo a gel (with more spacing) for a gel purification and subsequent PCR. This way we should have only the desired parts left.
+==PCR of LacIM from PHD1, PHD2 and PHD3==
+Using the primers designed by Alex with the Biobricks prefix and suffix, we maid a PCR of the PHD1, PHD2 and PHD3 plasmids to amplify the LacIM gene and have it as a Biobrick available for our construct.