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EPF-Lausanne/19 August 2008
From 2008.igem.org
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As the result on the last PCR ("checking all the parts") was unclear (or even negative) for several small parts, we do the PCR once more to check the results. | As the result on the last PCR ("checking all the parts") was unclear (or even negative) for several small parts, we do the PCR once more to check the results. | ||
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| + | Result: The parts are clearly visible this time. Only thing is that there are bands visible at around 5kbp. Therefore we will redo a gel (with more spacing) for a gel purification and subsequent PCR. This way we should have only the desired parts left. | ||
==PCR of LacIM from PHD1, PHD2 and PHD3== | ==PCR of LacIM from PHD1, PHD2 and PHD3== | ||
Using the primers designed by Alex with the Biobricks prefix and suffix, we maid a PCR of the PHD1, PHD2 and PHD3 plasmids to amplify the LacIM gene and have it as a Biobrick available for our construct. | Using the primers designed by Alex with the Biobricks prefix and suffix, we maid a PCR of the PHD1, PHD2 and PHD3 plasmids to amplify the LacIM gene and have it as a Biobrick available for our construct. | ||
Latest revision as of 16:20, 19 August 2008
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PCR of the small parts
As the result on the last PCR ("checking all the parts") was unclear (or even negative) for several small parts, we do the PCR once more to check the results.
Result: The parts are clearly visible this time. Only thing is that there are bands visible at around 5kbp. Therefore we will redo a gel (with more spacing) for a gel purification and subsequent PCR. This way we should have only the desired parts left.
PCR of LacIM from PHD1, PHD2 and PHD3
Using the primers designed by Alex with the Biobricks prefix and suffix, we maid a PCR of the PHD1, PHD2 and PHD3 plasmids to amplify the LacIM gene and have it as a Biobrick available for our construct.
