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User:University of Washington/19 August 2008
From 2008.igem.org
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-Sequence for 3A assembly came back. None were correct. All still contained P1010 part(on pSB3K3). (which was 1 bp different from the sequence post on part registry) Will probably try isolating digested DNA by running gel and do ligation from there. | -Sequence for 3A assembly came back. None were correct. All still contained P1010 part(on pSB3K3). (which was 1 bp different from the sequence post on part registry) Will probably try isolating digested DNA by running gel and do ligation from there. | ||
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| + | ==LuxR from pLac== | ||
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| + | -Retrieved data from the 25-well pLac assay. Results were great; Inhibition of GFP expression by glucose was stronger than the activation power of IPTG. Thus, indeed, the promoter behaves as desired. | ||
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Back to [[Team:University_of_Washington/Notebook#Notebook]] | Back to [[Team:University_of_Washington/Notebook#Notebook]] | ||
Latest revision as of 22:07, 22 August 2008
LuxR from AraC and TetR
-Sequence for 3A assembly came back. None were correct. All still contained P1010 part(on pSB3K3). (which was 1 bp different from the sequence post on part registry) Will probably try isolating digested DNA by running gel and do ligation from there.
LuxR from pLac
-Retrieved data from the 25-well pLac assay. Results were great; Inhibition of GFP expression by glucose was stronger than the activation power of IPTG. Thus, indeed, the promoter behaves as desired.
Back to Team:University_of_Washington/Notebook#Notebook
