Imperial College/19 August 2008
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|{{#calendar: title=Imperial_College |year=2008 | month=08}} | |{{#calendar: title=Imperial_College |year=2008 | month=08}} | ||
|{{#calendar: title=Imperial_College |year=2008 | month=09}} | |{{#calendar: title=Imperial_College |year=2008 | month=09}} | ||
- | | rowspan="2" bgcolor=# | + | | rowspan="2" bgcolor=#ffffff width="100%" | |
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= 19 August 2008 = | = 19 August 2008 = | ||
==Wetlab== | ==Wetlab== | ||
+ | |||
+ | ===Cloning=== | ||
+ | |||
+ | *GFP-3-mutB - Terminator and CAT containing XL1-BLues replica plated and overnight culture prepared | ||
+ | |||
+ | *Terminator, pSB1AK3 and mRFP - Terminator miniprepped from overnight cultures (all 5ml used) | ||
+ | **Single digests (''EcoRI'') run on a gel to determine approximate size | ||
+ | |||
===''B.subtilis''=== | ===''B.subtilis''=== | ||
- | *Transformation protocol 2 was repeated today, | + | |
+ | *Transformation protocol 2 was repeated today, [http://openwetware.org/wiki/IGEM:IMPERIAL/2008/Prototype/Wetlab/Transformation_protocol_4#Transformation_Protocol_2| Click link here for protocol]. The basic principle of this protocol is that competent cells are prepared by growing a culture to a high O.D.<sub>600</sub> and then performing electroporation on these competent cells. | ||
+ | *In previous attempts the competent cells were grown to an O.D.<sub>600</sub> of 1.5. Although this transformation worked, we wished to try to increase the O.D.<sub>600</sub> to increase the efficiency of transformation. The culture was grown to an O.D.<sub>600</sub> of 1.8 before being harvested. | ||
== Miscellaneous == | == Miscellaneous == | ||
- | * Krupa's birthday today! She's 19, for the third time | + | * Krupa's birthday today! She's 19, for the third time! |
+ | <br> | ||
+ | {{Imperial/EndPage|Notebook|Notebook}} |
Latest revision as of 20:37, 28 October 2008
19 August 2008WetlabCloning
B.subtilis
Miscellaneous
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