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EPF-Lausanne/20 August 2008
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| + | ==PCR of the small parts== | ||
| + | |||
| + | We found out that the primers used for PCR are very prominent in the gel. Therefore we cannot purify the small parts from it, as it is not possible to separate them. We will have to keep the small parts in the plasmid and add the other parts into it as was our initial plan. | ||
| + | |||
| + | ==Purification of LacIM== | ||
| + | |||
| + | After having controlled that the PCR of Ron Weiss's plasmids has worked, we did a gel purification of each form of LacIM separately. We then did another PCR of these products, which we will digest tomorrow. | ||
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| + | ==Ligations== | ||
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| + | The plate had a couple of colonies growing. We make new cell cultures using those colonies and let them grow overnight. | ||
Latest revision as of 07:56, 21 August 2008
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PCR of the small parts
We found out that the primers used for PCR are very prominent in the gel. Therefore we cannot purify the small parts from it, as it is not possible to separate them. We will have to keep the small parts in the plasmid and add the other parts into it as was our initial plan.
Purification of LacIM
After having controlled that the PCR of Ron Weiss's plasmids has worked, we did a gel purification of each form of LacIM separately. We then did another PCR of these products, which we will digest tomorrow.
Ligations
The plate had a couple of colonies growing. We make new cell cultures using those colonies and let them grow overnight.
