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Team:The University of Alberta/2 June 2008
From 2008.igem.org
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==To Do== | ==To Do== | ||
Design New Primers<br> | Design New Primers<br> | ||
Start Another Tray of Plants<br> | Start Another Tray of Plants<br> | ||
Test Gel Purifying Kit<br> | Test Gel Purifying Kit<br> | ||
| - | + | Protien Gels of the Butanol biobricks <br> | |
==Bad News== | ==Bad News== | ||
| - | The primers that we ordered for the Purple Russian and the Blue Ox that where supposed to contain the prefix and suffix do not contain a suffix. Sorry about that should have been double checked | + | The primers that we ordered for the Purple Russian and the Blue Ox that where supposed to contain the prefix and suffix do not contain a suffix. Sorry about that; should have been double checked |
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| + | '''EDIT:'''New primers have been designed, checked, double checked and triple checked. They should work this time. All of the other primers were not a total loss - the reverse primer for Blue Ox appeared to be correct. I think we'll order more with the new primers, though, just to make sure. -[[User:Cwk|Cwk]] 19:54, 2 June 2008 (UTC) | ||
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| + | ==Stuff We Did== | ||
| + | '''Chris''': | ||
| + | * Designed fancy new Buttons for the Project page in an attempt to get the Nav Bar to center. It didnt work but at least we now have some rather stylish buttons. | ||
| + | * Helped Winnie with colony PCR on all of the butanol BioBricks in both J61003 and I0500. The colonies were also streaked for SCIs with the exception of 23. I placed the colonies in the freezer (oops!) and 23 was streaked out afterwards; hopefully something survived so we'll get colonies. | ||
==Volunteer Questions From Saturday== | ==Volunteer Questions From Saturday== | ||
1. We really don't know why our gel purifications have such low concentrations, today we will run some tests to check and make sure our kit is still good<br> | 1. We really don't know why our gel purifications have such low concentrations, today we will run some tests to check and make sure our kit is still good<br> | ||
| - | 2.The reason I | + | 2.The reason I did not say to run a gel was because i was afriad that something was wrong with our purifying kit and I thought that we might just decrease our concintration more. Today however James corrected me saying that we should have run 10% of the digest saving the other 90% for ligation.<br> |
3. In this PCR we used PFU instead of the Homemade taq making our PCR more reliable and less error prone.<br> | 3. In this PCR we used PFU instead of the Homemade taq making our PCR more reliable and less error prone.<br> | ||
4. The maps of all vectors are up on the wiki in the parts registry.<br> | 4. The maps of all vectors are up on the wiki in the parts registry.<br> | ||
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| + | ==Lab Tip of the Day== | ||
| + | When meeting team mates for an unofficial pizza meeting always stand outside the restraunt to avoid being the only one in the restaraunt. | ||
Latest revision as of 23:22, 2 June 2008
Contents |
To Do
Design New Primers
Start Another Tray of Plants
Test Gel Purifying Kit
Protien Gels of the Butanol biobricks
Bad News
The primers that we ordered for the Purple Russian and the Blue Ox that where supposed to contain the prefix and suffix do not contain a suffix. Sorry about that; should have been double checked
EDIT:New primers have been designed, checked, double checked and triple checked. They should work this time. All of the other primers were not a total loss - the reverse primer for Blue Ox appeared to be correct. I think we'll order more with the new primers, though, just to make sure. -Cwk 19:54, 2 June 2008 (UTC)
Stuff We Did
Chris:
- Designed fancy new Buttons for the Project page in an attempt to get the Nav Bar to center. It didnt work but at least we now have some rather stylish buttons.
- Helped Winnie with colony PCR on all of the butanol BioBricks in both J61003 and I0500. The colonies were also streaked for SCIs with the exception of 23. I placed the colonies in the freezer (oops!) and 23 was streaked out afterwards; hopefully something survived so we'll get colonies.
Volunteer Questions From Saturday
1. We really don't know why our gel purifications have such low concentrations, today we will run some tests to check and make sure our kit is still good
2.The reason I did not say to run a gel was because i was afriad that something was wrong with our purifying kit and I thought that we might just decrease our concintration more. Today however James corrected me saying that we should have run 10% of the digest saving the other 90% for ligation.
3. In this PCR we used PFU instead of the Homemade taq making our PCR more reliable and less error prone.
4. The maps of all vectors are up on the wiki in the parts registry.
Lab Tip of the Day
When meeting team mates for an unofficial pizza meeting always stand outside the restraunt to avoid being the only one in the restaraunt.
