Alberta NINT/30 May 2008

From 2008.igem.org

(Difference between revisions)

Latest revision as of 22:12, 11 October 2008

lab work (SD):

         DNA from LB + amp50 culture tubes (from 29/05/08) was isolated using QIA prep standard miniprep 
         protocol.  
         This DNA was set up for sequencing. K102002.4 becomes K102002 and K102004.4 becomes K102004.

lab work (JD):

          Counted colonies on ligation plates: negative plate:0, Positive plate:2 
          because only two colonies grew we plated a second positive plate from our O/Ns and left it to grow

lab work (WM):

          Miniprepped 8 O/Ns
          Digested K102010/(EcoRI + PstI)
          Sequence with VR primer

@@ Line 1: / Line 1: @@
-lab work (SD): DNA from LB + amp50 culture tubes (from 29/05/08) was isolated using QIA prep standard miniprep protocol.  This DNA was set up for sequencing.
+[[Team:Alberta_NINT/Notebook | < Back to notebook]]
-lab work (JD): Counted colonies on ligation plates: negative plate:0, Positive plate:2 and because only two colonies grew we plated a second positive plate from our O/Ns and left it to grow
+[[Alberta_NINT/29_May_2008 | < Previous entry]] | [[Alberta_NINT/31_May_2008 | Next entry >]]
+lab work (SD):
+          DNA from LB + amp50 culture tubes (from 29/05/08) was isolated using QIA prep standard miniprep
+          protocol.
+          This DNA was set up for sequencing. K102002.4 becomes K102002 and K102004.4 becomes K102004.
+lab work (JD):
+           Counted colonies on ligation plates: negative plate:0, Positive plate:2
+           because only two colonies grew we plated a second positive plate from our O/Ns and left it to grow
+lab work (WM):
+           Miniprepped 8 O/Ns
+           Digested K102010/(EcoRI + PstI)
+           Sequence with VR primer
+           [[image: NINT_WMpg24b.JPG|500px]]