"
Team:BCCS-Bristol/Protocols-Media
From 2008.igem.org
(Difference between revisions)
Tgorochowski (Talk | contribs) (→LB broth or agar (200 ml)) |
Tgorochowski (Talk | contribs) |
||
| (4 intermediate revisions not shown) | |||
| Line 1: | Line 1: | ||
| - | <html><link rel="stylesheet" href="http:// | + | <html><link rel="stylesheet" href="http://homepage.mac.com/tgorochowski/iGEM/bccs-igem.css" type="text/css"></html> |
__NOTOC__ | __NOTOC__ | ||
<div class="bccsNavBar"> | <div class="bccsNavBar"> | ||
| Line 33: | Line 33: | ||
|- | |- | ||
|Agar||For plates 2||For plates 4 g | |Agar||For plates 2||For plates 4 g | ||
| - | |||
|} | |} | ||
| Line 42: | Line 41: | ||
| align="center" style="background:#f0f0f0;"|'''Concentration [%]''' | | align="center" style="background:#f0f0f0;"|'''Concentration [%]''' | ||
| align="center" style="background:#f0f0f0;"|'''Amount for 200 ml''' | | align="center" style="background:#f0f0f0;"|'''Amount for 200 ml''' | ||
| - | |||
| - | |||
|- | |- | ||
|Bactotryptone||1||2 g | |Bactotryptone||1||2 g | ||
| Line 49: | Line 46: | ||
|NaCl||0.5||2 g | |NaCl||0.5||2 g | ||
|- | |- | ||
| - | |Agar||various | + | |Agar||various|| |
| - | | | + | |
|} | |} | ||
| Line 58: | Line 54: | ||
| align="center" style="background:#f0f0f0;"|'''Concentration [%]''' | | align="center" style="background:#f0f0f0;"|'''Concentration [%]''' | ||
| align="center" style="background:#f0f0f0;"|'''Amount for 95 (100) ml''' | | align="center" style="background:#f0f0f0;"|'''Amount for 95 (100) ml''' | ||
| - | |||
| - | |||
|- | |- | ||
|Bactotryptone||2||2 g | |Bactotryptone||2||2 g | ||
| Line 66: | Line 60: | ||
|- | |- | ||
|NaCl||0.05||0.05 g | |NaCl||0.05||0.05 g | ||
| - | |||
|} | |} | ||
Mix the three components with 95 ml distilled water and autoclave it. Just before using, add (filter sterilized): | Mix the three components with 95 ml distilled water and autoclave it. Just before using, add (filter sterilized): | ||
| - | + | * 0.5 ml 2 M MgCl2 | |
| - | + | * 2 ml 1 M Glucose | |
| - | + | ||
| - | + | ||
<br> | <br> | ||
</div> | </div> | ||
Latest revision as of 09:48, 14 September 2008
Media
LB broth or agar (200 ml)
| Component | Concentration [%] | Amount for 200 ml |
| Yeast Extract | 0.5 | 1 g |
| Bactotryptone | 1 | 2 g |
| NaCl | 1 | 2 g |
| Agar | For plates 2 | For plates 4 g |
Swimming agar medium
| Component | Concentration [%] | Amount for 200 ml |
| Bactotryptone | 1 | 2 g |
| NaCl | 0.5 | 2 g |
| Agar | various |
SOC medium
| Component | Concentration [%] | Amount for 95 (100) ml |
| Bactotryptone | 2 | 2 g |
| Bacto yeast extract | 0.5 | 0.5 g |
| NaCl | 0.05 | 0.05 g |
Mix the three components with 95 ml distilled water and autoclave it. Just before using, add (filter sterilized):
- 0.5 ml 2 M MgCl2
- 2 ml 1 M Glucose
