"
Team:University of Lethbridge/Notebook/Project2September
From 2008.igem.org
(Difference between revisions)
Munima.alam (Talk | contribs) m (link to go back to the main notebook) |
Munima.alam (Talk | contribs) m (spacing) |
||
| (2 intermediate revisions not shown) | |||
| Line 1: | Line 1: | ||
[[Team:University_of_Lethbridge/Notebook|Back to The University of Lethbridge Main Notebook]] | [[Team:University_of_Lethbridge/Notebook|Back to The University of Lethbridge Main Notebook]] | ||
| + | |||
| + | ===September 1, 2008=== | ||
| + | ====Roxanne==== | ||
| + | -Picked the colonies which appear to have amplified during the screening PCR, and Incubated them in LB+amp media at 37.0C overnight. | ||
| + | |||
| + | |||
| + | ===September 2, 2008=== | ||
| + | ====Roxanne==== | ||
| + | -Plasmid Prepped the colonies which were incubated overnight. Ran Plasmids on a 1% Agarose Gel at 100V for 27 minutes. | ||
| + | |||
| + | ====Nathan Puhl==== | ||
| + | -Visulaized the Gel, and chose appropriate cells to glycerol stock. | ||
| + | |||
| + | |||
| + | ====September 3, 2008==== | ||
| + | ===Roxanne, Nathan Puhl=== | ||
| + | -Performed a Restriction Digest on RS1 and RS2 from pGEM T-easy using XbaI and SpeI. Ran overnight at 37.0C | ||
| + | |||
| + | ====Nathan==== | ||
| + | -Reran the PCR of the riboswtich from the primers and has been working on the Riboswitch throughout the month. | ||
| + | |||
| + | Gel of RS_cheZ and RS1, RS2 | ||
| + | |||
| + | [[Image:RS_cheZ.jpg|350 px]] | ||
| + | |||
| + | |||
| + | Gel of the New Riboswitch PCR product | ||
| + | |||
| + | [[Image:New_RS.jpg|150 px]] | ||
Latest revision as of 02:35, 30 October 2008
Back to The University of Lethbridge Main Notebook
Contents |
September 1, 2008
Roxanne
-Picked the colonies which appear to have amplified during the screening PCR, and Incubated them in LB+amp media at 37.0C overnight.
September 2, 2008
Roxanne
-Plasmid Prepped the colonies which were incubated overnight. Ran Plasmids on a 1% Agarose Gel at 100V for 27 minutes.
Nathan Puhl
-Visulaized the Gel, and chose appropriate cells to glycerol stock.
September 3, 2008
Roxanne, Nathan Puhl
-Performed a Restriction Digest on RS1 and RS2 from pGEM T-easy using XbaI and SpeI. Ran overnight at 37.0C
Nathan
-Reran the PCR of the riboswtich from the primers and has been working on the Riboswitch throughout the month.
Gel of RS_cheZ and RS1, RS2
Gel of the New Riboswitch PCR product
