User:University of Washington/27 August 2008
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==LuxR from AraC and TetR(Faifan)== | ==LuxR from AraC and TetR(Faifan)== | ||
- | + | <h4>3A ligation</h4> | |
+ | -miniprepped x 6 | ||
+ | |||
+ | -submit forward sequencing x 6 | ||
+ | |||
+ | <h4>with CIP reaction</h4> | ||
+ | |||
+ | - ran gel on digested promoter, GFP, and LuxR and cut bands(3184 bp for promoter vector, 898 bp for GFP insert, and 958 bp for LuxR insert) | ||
+ | |||
+ | - did gel purification | ||
+ | |||
+ | - nanodropped at 260 nm | ||
+ | <table border=1> | ||
+ | <tr align="center"> | ||
+ | <th></th><th>ng/ul</th><th>260/280</th><th>260/230</th> | ||
+ | </tr> | ||
+ | <tr align="center"> | ||
+ | <td>promoter vector</td><td>129.9</td><td>1.86</td><td>0.61</td> | ||
+ | </tr> | ||
+ | <tr align="center"> | ||
+ | <td>GFP</td><td>20.8</td><td>2.35</td><td>0.04</td> | ||
+ | </tr> | ||
+ | <tr align="center"> | ||
+ | <td>LuxR</td><td>11.6</td><td>1.98</td><td>0.05</td> | ||
+ | </tr> | ||
+ | </table> | ||
---- | ---- | ||
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Latest revision as of 23:17, 27 August 2008
LuxR from AraC and TetR(Faifan)
3A ligation
-miniprepped x 6
-submit forward sequencing x 6
with CIP reaction
- ran gel on digested promoter, GFP, and LuxR and cut bands(3184 bp for promoter vector, 898 bp for GFP insert, and 958 bp for LuxR insert)
- did gel purification
- nanodropped at 260 nm
ng/ul | 260/280 | 260/230 | |
---|---|---|---|
promoter vector | 129.9 | 1.86 | 0.61 |
GFP | 20.8 | 2.35 | 0.04 |
LuxR | 11.6 | 1.98 | 0.05 |
Back to Team:University_of_Washington/Notebook#Notebook