Template:Team:UC Berkeley/Notebook/CDB construction

From 2008.igem.org

(Difference between revisions)
(New page: PCR ca8373/ca8374 on pSB1A2-I13521 (1261bp, EcoRI/BamHI/DpnI) Sub into pBca1256 (EcoRI/BamHI, 2472+910, L) Product is pBca1256-K112800 ----------------------------...)
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PCR ca8373/ca8374 on pSB1A2-I13521    (1261bp, EcoRI/BamHI/DpnI)
+
__toc__
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Sub into pBca1256                     (EcoRI/BamHI, 2472+910, L)
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==pBca1256-K112400==
-
Product is pBca1256-K112800
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-
-----------------------------------------------
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<pre>
-
ca8373 Forward Biobricking of mRFP
+
Biobricking of promoter for grpE protein
-
CCATAGAATTCatgAGATCTatgatatttgatgctagtttaaag
+
PCR grpE-F and grpE-R on MG1655   (129 bp, EcoRI/BamHI)
-
ca8374 Reverse Biobricking of mRFP
+
Sub into pBca1256                   (EcoRI/BamHI 910+2472bp, L)
-
CGttaGGATCCttactcccccaagaaaatcc
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Product is pBca1256-K112400
 +
----------------------------
 +
cb0001-grpE-F Forward Biobricking    cgataGAATTCatgAGATCTccgaggtccttgttgcgaag
 +
cb0002-grpE-R Reverse Biobricking    cgtagGGATCCcgcattcatctcgctaacttc
 +
</pre>
 +
 
 +
==pBca1256-K112401==
 +
 
 +
<pre>
 +
Biobricking of promoter for recA protein
 +
PCR recA-F and recA-R on MG1655   (317 bp, EcoR1/BamH1)
 +
Sub into pBca1256                   (EcoR1/BamH1 910+2472bp, L)
 +
Product is pBca1256-K112401
 +
  ----------------------------
 +
cb0003-recA-F Forward Biobricking    cgataGAATTCatgAGATCTagagaagcctgtcggcaccg
 +
cb0004-recA-R Reverse Biobricking    cgtagGGATCCcccagtgctgccgccaacgc
 +
</pre>
 +
 
 +
==pBca1256-K112402==
 +
<pre>
 +
Biobricking of promoter for fabA protein
 +
PCR fabA-F and fabA-R on MG1655   (285 bp, EcoR1/BamH1)
 +
Sub into pBca1256                   (EcoR1/BamH1 910+2472bp, L)
 +
Product is pBca1256-K112402
 +
----------------------------
 +
cb0005-fabA-F Forward Biobricking  cgataGAATTCatgAGATCTggccattacgttggctgaac
 +
cb0006-fabA-R Reverse Biobricking  cgtagGGATCCgaagttaccacccgtttcgg
 +
</pre>
 +
 
 +
==pBca1256-K112403==
 +
<pre>
 +
Biobricking of signal peptide (prepro) of phoA (alkaline phosphatase)
 +
PCR cb10007-F and cb10008 on MG1655 (114 bp, EcoRI/BamHI)
 +
Sub into pBca1256         (EcoRI/BamHI 910+2472bp, L)                  
 +
Product is pBca1256-K112403
 +
----------------------------
 +
cb10007-phoA-F cgataGAATTCatgAGATCTgtacatggagaaaataaaatgaaacaaagc
 +
cb10008-phoA-R cgtagGGATCCgggcttttgtcacaggggtaaac
 +
</pre>
 +
 
 +
==pBca1256-K112404==
 +
<pre>
 +
Biobricking of signal peptide (prepro) of lamB protein
 +
PCR cb10009-F and cb10010 on MG1655 (132 bp, EcoRI/BamHI)
 +
Sub into pBca1256         (EcoRI/BamHI 910+2472bp, L)                  
 +
Product is pBca1256-K112404
 +
----------------------------
 +
cb10009-lamB-F cgataGAATTCatgAGATCTgaaaagcaatgactcaggagatag
 +
cb10010-lamB-R cgtagGGATCCcagccattgcctgagcagacattac
 +
</pre>
 +
 
 +
==pBca1256-K112405==
 +
<pre>
 +
Biobricking of promoter for cadAB genes
 +
PCR cad-F and cad-R on MG1655           (402 bp, EcoRI/BamHI)
 +
Sub into pBca1256                   (EcoRI/BamHI 910+2472bp, L)
 +
Product is pBca1256-K112405
 +
----------------------------
 +
cb10011-cad-F          cgataGAATTCatgAGATCTggtatattccagacttctgttcc
 +
cb10012-cad-R          cgtagGGATCCtgaccagaccaaactggcg
 +
</pre>
 +
 
 +
==pBca1256-K112406==
 +
<pre>
 +
Biobricking of cadC promoter and cadC gene
 +
PCR cadC-F and cadC-R on MG1655   (2336 bp, EcoR1/BamH1)
 +
Sub into pBca1256                   (EcoR1/BamH1 910+2472bp, L)
 +
Product is pBca1256-K112406
 +
----------------------------
 +
cb10013-cadC-F                cgataGAATTCatgAGATCTcaccagcaatttgcgggcatatc
 +
cb10012-cad-R                cgtagGGATCCtgaccagaccaaactggcg
 +
</pre>
 +
 
 +
==pBca1256-K112407==
 +
<pre>
 +
Biobricking of ygeF pseudogene promoter
 +
PCR ygeF-F and ygeF-R on MG1655   (540 bp, EcoR1/BamH1)
 +
Sub into pBca1256                   (EcoR1/BamH1 910+2472bp, L)
 +
Product is pBca1256-K112407
 +
----------------------------
 +
cb10014-ygeF-F      cgataGAATTCatgAGATCTcagtttactcagtgaatcaatagagg
 +
cb10015-ygeF-R      cgtagGGATCCatttgattagagcaaaatccCaattcgcagc
 +
</pre>
 +
 
 +
==pBjh1601CA-K112408==
 +
<pre>
 +
T4 derived lysis device
 +
Sub into pBjh1601KA     (5025 bp, EcoR1/BamH1)
 +
Product is pBjh1601KA-K112408      (EcoR1/BamH1 3196/1824 S)
 +
----------------------------
 +
</pre>

Latest revision as of 04:22, 24 October 2008

Contents

pBca1256-K112400

Biobricking of promoter for grpE protein 
 PCR grpE-F and grpE-R on MG1655 	  (129 bp, EcoRI/BamHI)
 Sub into pBca1256	                  (EcoRI/BamHI 910+2472bp, L)
 Product is pBca1256-K112400
 ----------------------------
cb0001-grpE-F Forward Biobricking    cgataGAATTCatgAGATCTccgaggtccttgttgcgaag
cb0002-grpE-R Reverse Biobricking    cgtagGGATCCcgcattcatctcgctaacttc

pBca1256-K112401

Biobricking of promoter for recA protein 
 PCR recA-F and recA-R on MG1655 	  (317 bp, EcoR1/BamH1)
 Sub into pBca1256	                  (EcoR1/BamH1 910+2472bp, L)
 Product is pBca1256-K112401
   ----------------------------
cb0003-recA-F Forward Biobricking    cgataGAATTCatgAGATCTagagaagcctgtcggcaccg
cb0004-recA-R Reverse Biobricking    cgtagGGATCCcccagtgctgccgccaacgc

pBca1256-K112402

Biobricking of promoter for fabA protein 
 PCR fabA-F and fabA-R on MG1655 	  (285 bp, EcoR1/BamH1)
 Sub into pBca1256	                  (EcoR1/BamH1 910+2472bp, L)
 Product is pBca1256-K112402
 ----------------------------
cb0005-fabA-F Forward Biobricking   cgataGAATTCatgAGATCTggccattacgttggctgaac 
cb0006-fabA-R Reverse Biobricking   cgtagGGATCCgaagttaccacccgtttcgg

pBca1256-K112403

Biobricking of signal peptide (prepro) of phoA (alkaline phosphatase) 
 PCR cb10007-F and cb10008 on MG1655	(114 bp, EcoRI/BamHI)
 Sub into pBca1256	        	(EcoRI/BamHI 910+2472bp, L)	                  
 Product is pBca1256-K112403
 ----------------------------
cb10007-phoA-F	cgataGAATTCatgAGATCTgtacatggagaaaataaaatgaaacaaagc
cb10008-phoA-R	cgtagGGATCCgggcttttgtcacaggggtaaac

pBca1256-K112404

Biobricking of signal peptide (prepro) of lamB protein 
 PCR cb10009-F and cb10010 on MG1655	(132 bp, EcoRI/BamHI)
 Sub into pBca1256	        	(EcoRI/BamHI 910+2472bp, L)	                  
 Product is pBca1256-K112404
 ----------------------------
cb10009-lamB-F 		cgataGAATTCatgAGATCTgaaaagcaatgactcaggagatag
cb10010-lamB-R 		cgtagGGATCCcagccattgcctgagcagacattac

pBca1256-K112405

Biobricking of promoter for cadAB genes 
 PCR cad-F and cad-R on MG1655 	          (402 bp, EcoRI/BamHI)
 Sub into pBca1256	                  (EcoRI/BamHI 910+2472bp, L)
 Product is pBca1256-K112405
 ----------------------------
cb10011-cad-F           cgataGAATTCatgAGATCTggtatattccagacttctgttcc
cb10012-cad-R           cgtagGGATCCtgaccagaccaaactggcg

pBca1256-K112406

Biobricking of cadC promoter and cadC gene
 PCR cadC-F and cadC-R on MG1655 	   (2336 bp, EcoR1/BamH1)
 Sub into pBca1256	                  (EcoR1/BamH1 910+2472bp, L)
 Product is pBca1256-K112406
 ----------------------------
cb10013-cadC-F                 cgataGAATTCatgAGATCTcaccagcaatttgcgggcatatc
cb10012-cad-R                 cgtagGGATCCtgaccagaccaaactggcg

pBca1256-K112407

Biobricking of ygeF pseudogene promoter
 PCR ygeF-F and ygeF-R on MG1655 	   (540 bp, EcoR1/BamH1)
 Sub into pBca1256	                  (EcoR1/BamH1 910+2472bp, L)
 Product is pBca1256-K112407
 ----------------------------
cb10014-ygeF-F      cgataGAATTCatgAGATCTcagtttactcagtgaatcaatagagg
cb10015-ygeF-R      cgtagGGATCCatttgattagagcaaaatccCaattcgcagc

pBjh1601CA-K112408

T4 derived lysis device
 Sub into pBjh1601KA		    (5025 bp, EcoR1/BamH1)
 Product is pBjh1601KA-K112408      (EcoR1/BamH1 3196/1824 S)
----------------------------
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