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EPF-Lausanne/28 August 2008
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| + | [https://2008.igem.org/wiki/index.php?title=EPF-Lausanne/27_August_2008 <<Previous] - [https://2008.igem.org/Team:EPF-Lausanne/Notebook Back to Notebook] - | ||
| + | [https://2008.igem.org/wiki/index.php?title=EPF-Lausanne/29_August_2008 Next>>] | ||
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Ligation of the day : | Ligation of the day : | ||
- F1 = C0070 (insert) + B0015 (vector) for the 2nd plasmid. | - F1 = C0070 (insert) + B0015 (vector) for the 2nd plasmid. | ||
| + | |||
- B1 = B0034 (vector) + LacIm (Insert) | - B1 = B0034 (vector) + LacIm (Insert) | ||
| - | - | + | |
| + | - A2 = A1 (Insert) + B0034 (Vector) -> will not be used | ||
| + | |||
| + | - D2 = D1 (Insert) + RFP E1010 (Vector) | ||
PCR of Plac from pHD1. | PCR of Plac from pHD1. | ||
New digestion : Plac with XP (insert), B0015 with SP (Vector) | New digestion : Plac with XP (insert), B0015 with SP (Vector) | ||
| + | |||
| + | ---- | ||
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| + | Test surface chemistry for MITOMI experiment : | ||
| + | |||
| + | -> success / GFP bounded under button | ||
Latest revision as of 15:39, 26 October 2008
<<Previous - Back to Notebook - Next>>
Ligation of the day :
- F1 = C0070 (insert) + B0015 (vector) for the 2nd plasmid.
- B1 = B0034 (vector) + LacIm (Insert)
- A2 = A1 (Insert) + B0034 (Vector) -> will not be used
- D2 = D1 (Insert) + RFP E1010 (Vector)
PCR of Plac from pHD1. New digestion : Plac with XP (insert), B0015 with SP (Vector)
Test surface chemistry for MITOMI experiment :
-> success / GFP bounded under button
