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EPF-Lausanne/28 August 2008
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- B1 = B0034 (vector) + LacIm (Insert) | - B1 = B0034 (vector) + LacIm (Insert) | ||
| - | - A2 = A1 (Insert) + B0034 (Vector) | + | - A2 = A1 (Insert) + B0034 (Vector) -> will not be used |
- D2 = D1 (Insert) + RFP E1010 (Vector) | - D2 = D1 (Insert) + RFP E1010 (Vector) | ||
| Line 15: | Line 15: | ||
PCR of Plac from pHD1. | PCR of Plac from pHD1. | ||
New digestion : Plac with XP (insert), B0015 with SP (Vector) | New digestion : Plac with XP (insert), B0015 with SP (Vector) | ||
| + | |||
| + | ---- | ||
| + | |||
| + | Test surface chemistry for MITOMI experiment : | ||
| + | |||
| + | -> success / GFP bounded under button | ||
Latest revision as of 15:39, 26 October 2008
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Ligation of the day :
- F1 = C0070 (insert) + B0015 (vector) for the 2nd plasmid.
- B1 = B0034 (vector) + LacIm (Insert)
- A2 = A1 (Insert) + B0034 (Vector) -> will not be used
- D2 = D1 (Insert) + RFP E1010 (Vector)
PCR of Plac from pHD1. New digestion : Plac with XP (insert), B0015 with SP (Vector)
Test surface chemistry for MITOMI experiment :
-> success / GFP bounded under button
