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Team:KULeuven/30 August 2008
From 2008.igem.org
(New page: {{:Team:KULeuven/Tools/Header}} == Lab Work == === Wet Lab === === Dry Lab === Created the oligos for the B1002 and B1006 terminators [http://partsregistry.org/cgi/partsdb/pgroup.cgi?pg...) |
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== Lab Work == | == Lab Work == | ||
=== Wet Lab === | === Wet Lab === | ||
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| + | A glycerol stock was made of the new parts and of our own parts ([http://partsregistry.org/Part:BBa_K145151 K145151], [http://partsregistry.org/Part:BBa_K145001 K145001], [http://partsregistry.org/Part:BBa_K145015 K145015]). We forgot one, so a liquid culture was made of part [http://partsregistry.org/Part:BBa_R0053 R0053]. | ||
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| + | We miniprepped the new parts and the two ligations that succeeded ([http://partsregistry.org/Part:BBa_C0040 C0040]+[http://partsregistry.org/Part:BBa_B0015 B0015] and [http://partsregistry.org/Part:BBa_C0060 C0060]+[http://partsregistry.org/Part:BBa_B0015 B0015] that were plated on Ap instead of Km). They were then cut with ''Xba''I. | ||
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| + | The digests with ''Xba''I were started: [http://partsregistry.org/Part:BBa_B0015 B0015], [http://partsregistry.org/Part:BBa_B0033 B0033], [http://partsregistry.org/Part:BBa_B0034 B0034], [http://partsregistry.org/Part:BBa_J23066 J23066], [http://partsregistry.org/Part:BBa_P0353 P0353], [http://partsregistry.org/Part:BBa_P0152 P0152], [http://partsregistry.org/Part:BBa_E0240 E0240]. | ||
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| + | The ligations we made yesterday were electroporated today (still in electrocompetent Top10 cells): [http://partsregistry.org/Part:BBa_R0011 R0011]+[http://partsregistry.org/Part:BBa_B0032 B0032], [http://partsregistry.org/Part:BBa_C0040 C0040]+[http://partsregistry.org/Part:BBa_B0015 B0015], [http://partsregistry.org/Part:BBa_C0060 C0060]+[http://partsregistry.org/Part:BBa_B0015 B0015], [http://partsregistry.org/Part:BBa_C0012 C0012]+[http://partsregistry.org/Part:BBa_B0015 B0015], [http://partsregistry.org/Part:BBa_K145015 K145015]+[http://partsregistry.org/Part:BBa_B0015 B0015], [http://partsregistry.org/Part:BBa_K145001 K145001]+[http://partsregistry.org/Part:BBa_B0015 B0015], [http://partsregistry.org/Part:BBa_K145151 K145151]+[http://partsregistry.org/Part:BBa_B0015 B0015] and ([http://partsregistry.org/Part:BBa_R0040 R0040]+[http://partsregistry.org/Part:BBa_J23022 J23022])+([http://partsregistry.org/Part:BBa_J23109 J23109]+[http://partsregistry.org/Part:BBa_J23032 J23032]). | ||
=== Dry Lab === | === Dry Lab === | ||
| + | |||
| + | ==== General ==== | ||
Created the oligos for the B1002 and B1006 terminators [http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=terminator link] | Created the oligos for the B1002 and B1006 terminators [http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=terminator link] | ||
==== Modeling ==== | ==== Modeling ==== | ||
Final updated model uploaded to the wiki (only MatLab) | Final updated model uploaded to the wiki (only MatLab) | ||
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== Remarks == | == Remarks == | ||
| - | + | I don't think the dam methylation is the problem with our ligations. This is the prefix: | |
| - | + | gaattcgcggccgct'''tctaga'''g. The recognition site of ''Xba''I is in bold. There is no overlap for dam methylation (gatc). | |
Latest revision as of 15:30, 12 October 2008
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Contents |
Lab Work
Wet Lab
A glycerol stock was made of the new parts and of our own parts ([http://partsregistry.org/Part:BBa_K145151 K145151], [http://partsregistry.org/Part:BBa_K145001 K145001], [http://partsregistry.org/Part:BBa_K145015 K145015]). We forgot one, so a liquid culture was made of part [http://partsregistry.org/Part:BBa_R0053 R0053].
We miniprepped the new parts and the two ligations that succeeded ([http://partsregistry.org/Part:BBa_C0040 C0040]+[http://partsregistry.org/Part:BBa_B0015 B0015] and [http://partsregistry.org/Part:BBa_C0060 C0060]+[http://partsregistry.org/Part:BBa_B0015 B0015] that were plated on Ap instead of Km). They were then cut with XbaI.
The digests with XbaI were started: [http://partsregistry.org/Part:BBa_B0015 B0015], [http://partsregistry.org/Part:BBa_B0033 B0033], [http://partsregistry.org/Part:BBa_B0034 B0034], [http://partsregistry.org/Part:BBa_J23066 J23066], [http://partsregistry.org/Part:BBa_P0353 P0353], [http://partsregistry.org/Part:BBa_P0152 P0152], [http://partsregistry.org/Part:BBa_E0240 E0240].
The ligations we made yesterday were electroporated today (still in electrocompetent Top10 cells): [http://partsregistry.org/Part:BBa_R0011 R0011]+[http://partsregistry.org/Part:BBa_B0032 B0032], [http://partsregistry.org/Part:BBa_C0040 C0040]+[http://partsregistry.org/Part:BBa_B0015 B0015], [http://partsregistry.org/Part:BBa_C0060 C0060]+[http://partsregistry.org/Part:BBa_B0015 B0015], [http://partsregistry.org/Part:BBa_C0012 C0012]+[http://partsregistry.org/Part:BBa_B0015 B0015], [http://partsregistry.org/Part:BBa_K145015 K145015]+[http://partsregistry.org/Part:BBa_B0015 B0015], [http://partsregistry.org/Part:BBa_K145001 K145001]+[http://partsregistry.org/Part:BBa_B0015 B0015], [http://partsregistry.org/Part:BBa_K145151 K145151]+[http://partsregistry.org/Part:BBa_B0015 B0015] and ([http://partsregistry.org/Part:BBa_R0040 R0040]+[http://partsregistry.org/Part:BBa_J23022 J23022])+([http://partsregistry.org/Part:BBa_J23109 J23109]+[http://partsregistry.org/Part:BBa_J23032 J23032]).
Dry Lab
General
Created the oligos for the B1002 and B1006 terminators [http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=terminator link]
Modeling
Final updated model uploaded to the wiki (only MatLab)
Remarks
I don't think the dam methylation is the problem with our ligations. This is the prefix: gaattcgcggccgcttctagag. The recognition site of XbaI is in bold. There is no overlap for dam methylation (gatc).
