Alberta NINT/21 August 2008

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(Difference between revisions)

Latest revision as of 23:10, 11 October 2008

lab work (SD):

            Ligated TA2+(PCR)/B+N (NR) into K102031/B+N (GP).
            Transformed into XL1-B cells and incubated overnight at 37 C.

lab work (JD):

             Digested mini-prep of cotransformed cells with EcoR1
                   -to run on gel and check if in fact both plasmids inside the cell are low copy 
                    plasmids, bands should be about the same brightness
             Ran on gel and found that the band intensities were not the same.
             
             Analyzed LacZ Assay Data
             Media:Alberta_NINT_LacZ_assay_August_22_08.xls

@@ Line 1: / Line 1: @@
 [[Team:Alberta_NINT/Notebook | < Back to notebook]]
-[[Alberta_NINT/20_August_2008 | < Previous entry]]
+[[Alberta_NINT/20_August_2008 | < Previous entry]] | [[Alberta_NINT/22_August_2008 | Next entry >]]
 lab work (SD):
@@ Line 9: / Line 10: @@
 lab work (JD):
                Digested mini-prep of cotransformed cells with EcoR1
-                          -to run on gel and check if in fact both plasmids inside the cell are low copy plasmids, bands should be about the same brightness
+                    -to run on gel and check if in fact both plasmids inside the cell are low copy
-               Ran on gel
+                     plasmids, bands should be about the same brightness
+               Ran on gel and found that the band intensities were not the same.
+              [[Image:UVP0343.jpg]]
                Analyzed LacZ Assay Data
                [[Media:Alberta_NINT_LacZ_assay_August_22_08.xls]]
-[[Alberta_NINT/22_August_2008 | Next entry >]]