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Alberta NINT/21 August 2008
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[[Team:Alberta_NINT/Notebook | < Back to notebook]] | [[Team:Alberta_NINT/Notebook | < Back to notebook]] | ||
| - | [[Alberta_NINT/20_August_2008 | < Previous entry]] | + | [[Alberta_NINT/20_August_2008 | < Previous entry]] | [[Alberta_NINT/22_August_2008 | Next entry >]] |
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lab work (SD): | lab work (SD): | ||
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lab work (JD): | lab work (JD): | ||
Digested mini-prep of cotransformed cells with EcoR1 | Digested mini-prep of cotransformed cells with EcoR1 | ||
| - | + | -to run on gel and check if in fact both plasmids inside the cell are low copy | |
| + | plasmids, bands should be about the same brightness | ||
Ran on gel and found that the band intensities were not the same. | Ran on gel and found that the band intensities were not the same. | ||
| - | [[Image: | + | [[Image:UVP0343.jpg]] |
Analyzed LacZ Assay Data | Analyzed LacZ Assay Data | ||
[[Media:Alberta_NINT_LacZ_assay_August_22_08.xls]] | [[Media:Alberta_NINT_LacZ_assay_August_22_08.xls]] | ||
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Latest revision as of 23:10, 11 October 2008
< Previous entry | Next entry >
lab work (SD):
Ligated TA2+(PCR)/B+N (NR) into K102031/B+N (GP).
Transformed into XL1-B cells and incubated overnight at 37 C.
lab work (JD):
Digested mini-prep of cotransformed cells with EcoR1
-to run on gel and check if in fact both plasmids inside the cell are low copy
plasmids, bands should be about the same brightness
Ran on gel and found that the band intensities were not the same.
Analyzed LacZ Assay Data
Media:Alberta_NINT_LacZ_assay_August_22_08.xls
