Template:Team:UC Berkeley/Notebook/Molly construction

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== Molly's Construction Files ==
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[[Team:UC_Berkeley/Notebook/Molly_Allen|Molly's Notebook]]<br>
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<pre>
<pre>
Construction of {xis!} Biobrick Part K112200
Construction of {xis!} Biobrick Part K112200
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mea001 Forward Biobricking of {xis!} ccataGAATTCatgAGATCTatgtacttgacacttcaggag
mea001 Forward Biobricking of {xis!} ccataGAATTCatgAGATCTatgtacttgacacttcaggag
-
mea002 Reverse Biobricking of {xis!} cgttaGGATCCtcatgacttcgccttcttcc
+
mea002 Reverse Biobricking of {xis!} cgttaGGATCCtcatgacttcgccttcttccc
</pre>
</pre>
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mea003 Forward Biobricking of {<xis!} ccataGAATTCatgAGATCTtacttgacacttcaggagtg
mea003 Forward Biobricking of {<xis!} ccataGAATTCatgAGATCTtacttgacacttcaggagtg
-
mea002 Reverse Biobricking of {xis!} cgttaGGATCCtcatgacttcgccttcttcc
+
mea002 Reverse Biobricking of {xis!} cgttaGGATCCtcatgacttcgccttcttccc
</pre>
</pre>
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Product is pBca1256-K112204
Product is pBca1256-K112204
----------------------------------------
----------------------------------------
-
mea017 Forward Biobricking of {a~xis!}ccataGAATTCatgAGATCTtgcgatgtacttgacacttcagg
+
mea017 Forward Biobricking of {a~xis!}ccagtGAATTCatgAGATCTtgcgatgtacttgacacttcagg
-
mea002 Reverse Biobricking of {xis!} cgttaGGATCCtcatgacttcgccttcttcc
+
mea002 Reverse Biobricking of {xis!} cgttaGGATCCtcatgacttcgccttcttccc
</pre>
</pre>
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Product is pBca1256-K112205
Product is pBca1256-K112205
----------------------------------------
----------------------------------------
-
mea018 Forward Biobricking of {rbs.xis!}ccataGAATTCatgAGATCTtaattgcggagactttgcgatg
+
mea018 Forward Biobricking of {rbs.xis!}ccagtGAATTCatgAGATCTtaattgcggagactttgcgatg
-
mea002 Reverse Biobricking of {xis!} cgttaGGATCCtgacttcgccttcttcc
+
mea002 Reverse Biobricking of {xis!} cgttaGGATCCtcatgacttcgccttcttccc
</pre>
</pre>
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----------------------------------------
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mea005 Forward Biobricking of {int!} ccataGAATTCatgAGATCTatgggaagaaggcgaagtcatg
mea005 Forward Biobricking of {int!} ccataGAATTCatgAGATCTatgggaagaaggcgaagtcatg
-
mea006 Reverse Biobricking of {int!} cgttaGGATCCttatttgatttcaattttgtcccactc
+
mea006 Reverse Biobricking of {int!} cgttaGGATCCttatttgatttcaattttgtcccactccc
</pre>
</pre>
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----------------------------------------
----------------------------------------
mea007 Forward Biobricking of {<int!} ccataGAATTCatgAGATCTggaagaaggcgaagtcatgagc
mea007 Forward Biobricking of {<int!} ccataGAATTCatgAGATCTggaagaaggcgaagtcatgagc
-
mea006 Reverse Biobricking of {int!} cgttaGGATCCttatttgatttcaattttgtcccactc
+
mea006 Reverse Biobricking of {int!} cgttaGGATCCttatttgatttcaattttgtcccactccc
</pre>
</pre>
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----------------------------------------
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mea019 Forward Biobricking of {a~int!}ccataGAATTCatgAGATCTagaaatgggaagaaggcgaag
mea019 Forward Biobricking of {a~int!}ccataGAATTCatgAGATCTagaaatgggaagaaggcgaag
-
mea006 Reverse Biobricking of {int!} cgttaGGATCCttatttgatttcaattttgtcccactc
+
mea006 Reverse Biobricking of {int!} cgttaGGATCCttatttgatttcaattttgtcccactccc
</pre>
</pre>
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----------------------------------------
----------------------------------------
mea020 Forward Biobricking of {rbs.int!}ccataGAATTCatgAGATCTttttgaagaggatcagaaatggg
mea020 Forward Biobricking of {rbs.int!}ccataGAATTCatgAGATCTttttgaagaggatcagaaatggg
-
mea006 Reverse Biobricking of {int!} cgttaGGATCCttatttgatttcaattttgtcccactc
+
mea006 Reverse Biobricking of {int!} cgttaGGATCCttatttgatttcaattttgtcccactccc
</pre>
</pre>
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mea009 Forward Biobricking of {ihfA!}ccataGAATTCatgAGATCTatggcgcttacaaaagctgaaatg
mea009 Forward Biobricking of {ihfA!}ccataGAATTCatgAGATCTatggcgcttacaaaagctgaaatg
-
mea010 Reverse Biobricking of {ihfA!} cgttaGGATCCttactcgtctttgggcgaagc
+
mea010 Reverse Biobricking of {ihfA!} cgttaGGATCCttactcgtctttgggcgaagcg
</pre>
</pre>
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mea011 Forward Biobricking of {<ihfA!}ccataGAATTCatgAGATCTgcgcttacaaaagctgaaatgtc
mea011 Forward Biobricking of {<ihfA!}ccataGAATTCatgAGATCTgcgcttacaaaagctgaaatgtc
-
mea010 Reverse Biobricking of {ihfA!} cgttaGGATCCttactcgtctttgggcgaagc
+
mea010 Reverse Biobricking of {ihfA!} cgttaGGATCCttactcgtctttgggcgaagcg
</pre>
</pre>
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----------------------------------------
mea021 Forward Biobricking of {a~ihfA!}ccataGAATTCatgAGATCTacctatggcgcttacaaaagc
mea021 Forward Biobricking of {a~ihfA!}ccataGAATTCatgAGATCTacctatggcgcttacaaaagc
-
mea010 Reverse Biobricking of {ihfA!} cgttaGGATCCttactcgtctttgggcgaagc
+
mea010 Reverse Biobricking of {ihfA!} cgttaGGATCCttactcgtctttgggcgaagcg
</pre>
</pre>
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Product is pBca1256-K112218
Product is pBca1256-K112218
----------------------------------------
----------------------------------------
-
mea022 Forward Biobricking of {rbs.ihfA!}ccataGAATTCatgAGATCTtcattgagggattgaacctatgg
+
mea022 Forward Biobricking of {rbs.ihfA!}ccataGAATTCatgAGATCTtcattgagggattgaacctatggcgc
-
mea010 Reverse Biobricking of {ihfA!} cgttaGGATCCttactcgtctttgggcgaagc
+
mea010 Reverse Biobricking of {ihfA!} cgttaGGATCCttactcgtctttgggcgaagcg
</pre>
</pre>
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Product is pBca1256-K112219
Product is pBca1256-K112219
----------------------------------------
----------------------------------------
-
mea013 Forward Biobricking of {ihfB!}ccataGAATTCatgAGATCTatgaccaagtcagaattgatagaaagac
+
mea013 Forward Biobricking of {ihfB!} ccataGAATTCatgAGATCTatgaccaagtcagaattgatagaaagacttgccaccc
mea014 Reverse Biobricking of {ihfB!} cgttaGGATCCttaaccgtaaatattggcgcgatcg
mea014 Reverse Biobricking of {ihfB!} cgttaGGATCCttaaccgtaaatattggcgcgatcg
</pre>
</pre>
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Product is pBca1256-K112220
Product is pBca1256-K112220
----------------------------------------
----------------------------------------
-
mea015 Forward Biobricking of {<ihfB!}ccataGAATTCatgAGATCTaccaagtcagaattgatagaaagac
+
mea015 Forward Biobricking of {<ihfB!} ccataGAATTCatgAGATCTaccaagtcagaattgatagaaagacttgccaccc
mea014 Reverse Biobricking of {ihfB!} cgttaGGATCCttaaccgtaaatattggcgcgatcg
mea014 Reverse Biobricking of {ihfB!} cgttaGGATCCttaaccgtaaatattggcgcgatcg
</pre>
</pre>
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Product is pBca1256-K112221
Product is pBca1256-K112221
----------------------------------------
----------------------------------------
-
mea013 Forward Biobricking of {ihfB!}ccataGAATTCatgAGATCTatgaccaagtcagaattgatagaaagac
+
mea013 Forward Biobricking of {ihfB!} ccataGAATTCatgAGATCTatgaccaagtcagaattgatagaaagacttgccaccc
mea016 Reverse Biobricking of {ihfB>} cgttaGGATCCaccgtaaatattggcgcgatc
mea016 Reverse Biobricking of {ihfB>} cgttaGGATCCaccgtaaatattggcgcgatc
</pre>
</pre>
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Product is pBca1256-K112222
Product is pBca1256-K112222
----------------------------------------
----------------------------------------
-
mea015 Forward Biobricking of {<ihfB!} ccataGAATTCatgAGATCTaccaagtcagaattgatagaaagac mea016 Reverse Biobricking of {ihfB>} cgttaGGATCCaccgtaaatattggcgcgatc
+
mea015 Forward Biobricking of {<ihfB!} ccataGAATTCatgAGATCTaccaagtcagaattgatagaaagacttgccaccc
 +
mea016 Reverse Biobricking of {ihfB>} cgttaGGATCCaccgtaaatattggcgcgatc
</pre>
</pre>
<pre>
<pre>
Construction of {a~ihfB!} Biobrick Part K112223
Construction of {a~ihfB!} Biobrick Part K112223
-
PCR mea023/mea014 on MG1655 (310 bp, EcoRI/BamHI/DpnI)
+
PCR mea023/mea014 on MG1655 (320 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256 (EcoRI/BamHI, 2472+910, L)
Sub into pBca1256 (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112223
Product is pBca1256-K112223
----------------------------------------
----------------------------------------
-
mea023 Forward Biobricking of {a~ihfB!} ccataGAATTCatgAGATCTaatcatgaccaagtcagaattgatag
+
mea023 Forward Biobricking of {a~ihfB!}ccataGAATTCatgAGATCTaatcatgaccaagtcagaattgatag
mea014 Reverse Biobricking of {ihfB!} cgttaGGATCCttaaccgtaaatattggcgcgatcg
mea014 Reverse Biobricking of {ihfB!} cgttaGGATCCttaaccgtaaatattggcgcgatcg
</pre>
</pre>
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Product is pBca1256-K112224
Product is pBca1256-K112224
----------------------------------------
----------------------------------------
-
mea024 Forward Biobricking of {rbs.ihfB!} ccataGAATTCatgAGATCTctttaaggaaccggaggaatcatg
+
mea024 Forward Biobricking of {rbs.ihfB!}ccataGAATTCatgAGATCTctttaaggaaccggaggaatcatg
mea014 Reverse Biobricking of {ihfB!} cgttaGGATCCttaaccgtaaatattggcgcgatcg
mea014 Reverse Biobricking of {ihfB!} cgttaGGATCCttaaccgtaaatattggcgcgatcg
</pre>
</pre>
 +
 +
<pre>
 +
Construction of {ompT>} Biobrick Part K112225
 +
Wobble PCR mea025/mea026 (97 bp, EcoRI/BamHI/DpnI)
 +
Sub into pBca1256 (EcoRI/BamHI, 2472+910, L)
 +
Product is pBca1256-K112225
 +
----------------------------------------
 +
mea025 Forward Biobricking of {ompT>} cgataGAATTCatgAGATCTatgcgggcgaaactcctaggaatagtcctgacaacccctatcg
 +
mea026 Reverse Biobricking of {ompT>} cgttaGGATCCcgtcgacgcaaaagagctgatcgcgataggggttgtcaggactattc
 +
</pre>
 +
 +
<pre>
 +
Construction of {a~ompT} Biobrick Part K112226
 +
Wobble PCR mea027/mea026 (101 bp, EcoRI/BamHI/DpnI)
 +
Sub into pBca1256 (EcoRI/BamHI, 2472+910, L)
 +
Product is pBca1256-K112226
 +
----------------------------------------
 +
mea027 Forward Biobricking of {a~ompT} cgataGAATTCatgAGATCTacatatgcgggcgaaactcctaggaatagtcctgacaacccctatc
 +
mea026 Reverse Biobricking of {ompT>} cgttaGGATCCcgtcgacgcaaaagagctgatcgcgataggggttgtcaggactattc
 +
</pre>
 +
 +
<pre>
 +
Construction of {rbs.ompT} Biobrick Part K112227
 +
Wobble PCR mea028/mea026 (114 bp, EcoRI/BamHI/DpnI)
 +
Sub into pBca1256 (EcoRI/BamHI, 2472+910, L)
 +
Product is pBca1256-K112227
 +
----------------------------------------
 +
mea028 Forward Biobricking of {rbs.ompT}cgataGAATTCatgAGATCTaagaaggagatatacatatgcgggcgaaactcctaggaatagtcctgacaacccc
 +
mea026 Reverse Biobricking of {ompT>} cgttaGGATCCcgtcgacgcaaaagagctgatcgcgataggggttgtcaggactattc
 +
</pre>
 +
 +
<pre>
 +
Construction of {pelB>} Biobrick Part K112228
 +
Wobble PCR mea029/mea030 (97 bp, EcoRI/BamHI/DpnI)
 +
Sub into pBca1256 (EcoRI/BamHI, 2472+910, L)
 +
Product is pBca1256-K112228
 +
----------------------------------------
 +
mea029 Forward Biobricking of {pelB>} cgataGAATTCatgAGATCTatgaaatacctgctgccgaccgctgctgctggtctgctgctcc
 +
mea030 Reverse Biobricking of {pelB>} cgttaGGATCCggccatcgccggctgggcagcgaggagcagcagaccagcagcagcgg
 +
</pre>
 +
 +
<pre>
 +
Construction of {a~pelB} Biobrick Part K112229
 +
Wobble PCR mea031/mea030 (101 bp, EcoRI/BamHI/DpnI)
 +
Sub into pBca1256 (EcoRI/BamHI, 2472+910, L)
 +
Product is pBca1256-K112229
 +
----------------------------------------
 +
mea031 Forward Biobricking of {a~pelB} cgataGAATTCatgAGATCTacatatgaaatacctgctgccgaccgctgctgctggtctgctg
 +
mea030 Reverse Biobricking of {pelB>} cgttaGGATCCggccatcgccggctgggcagcgaggagcagcagaccagcagcagcgg
 +
</pre>
 +
 +
<pre>
 +
Construction of {rbs.pelB} Biobrick Part K112230
 +
Wobble PCR mea032/mea030 (116 bp, EcoRI/BamHI/DpnI)
 +
Sub into pBca1256 (EcoRI/BamHI, 2472+910, L)
 +
Product is pBca1256-K112230
 +
----------------------------------------
 +
mea032 Forward Biobricking of {rbs.pelB} cgataGAATTCatgAGATCTttaagaaggagatatacatatgaaatacctgctgccgaccgctgctgctggtctgctg
 +
mea030 Reverse Biobricking of {pelB>} cgttaGGATCCggccatcgccggctgggcagcgaggagcagcagaccagcagcagcgg
 +
</pre>
 +
 +
<pre>
 +
Construction of {rbs.xis.int!} Biobrick Part K112234
 +
PCR mea018/mea006 on pAH57         (1317 bp, EcoRI/BamHI/DpnI)
 +
Sub into pBca1256 (EcoRI/BamHI, 2472+910, L)
 +
Product is pBca1256-K112234
 +
----------------------------------------
 +
mea018 Forward Biobricking of {rbs.xis!}ccagtGAATTCatgAGATCTtaattgcggagactttgcgatg
 +
mea006 Reverse Biobricking of {int!} cgttaGGATCCttatttgatttcaattttgtcccactccc
 +
</pre>
 +
 +
 +
[[Team:UC_Berkeley/Notebook/Molly_Allen|Molly's Notebook]]<br>

Latest revision as of 00:06, 4 August 2008

Molly's Construction Files

Molly's Notebook

Construction of {xis!} Biobrick Part K112200
PCR mea001/mea002 on pAH57		(230 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256			(EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112200
----------------------------------------
mea001	Forward Biobricking of {xis!}	ccataGAATTCatgAGATCTatgtacttgacacttcaggag
mea002	Reverse Biobricking of {xis!}	cgttaGGATCCtcatgacttcgccttcttccc

Construction of {<xis!} Biobrick Part K112201
PCR mea003/mea002 on pAH57		(227 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256			(EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112201
----------------------------------------
mea003	Forward Biobricking of {<xis!}	ccataGAATTCatgAGATCTtacttgacacttcaggagtg
mea002	Reverse Biobricking of {xis!}	cgttaGGATCCtcatgacttcgccttcttccc

Construction of {xis>} Biobrick Part K112202
PCR mea001/mea004 on pAH57		(227 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256			(EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112202
----------------------------------------
mea001	Forward Biobricking of {xis!}	ccataGAATTCatgAGATCTatgtacttgacacttcaggag
mea004	Reverse Biobricking of {xis>}	cgttaGGATCCtgacttcgccttcttcccatttc

Construction of {<xis>} Biobrick Part K112203
PCR mea003/mea004 on pAH57		(224 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256			(EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112203
----------------------------------------
mea003	Forward Biobricking of {<xis!}	ccataGAATTCatgAGATCTtacttgacacttcaggagtg
mea004	Reverse Biobricking of {xis>}	cgttaGGATCCtgacttcgccttcttcccatttc

Construction of {a~xis!} Biobrick Part K112204
PCR mea017/mea002 on pAH57		(234 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256			(EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112204
----------------------------------------
mea017	Forward Biobricking of {a~xis!}ccagtGAATTCatgAGATCTtgcgatgtacttgacacttcagg
mea002	Reverse Biobricking of {xis!}	cgttaGGATCCtcatgacttcgccttcttccc

Construction of {rbs.xis!} Biobrick Part K112205
PCR mea018/mea002 on pAH57		(249 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256			(EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112205
----------------------------------------
mea018	Forward Biobricking of {rbs.xis!}ccagtGAATTCatgAGATCTtaattgcggagactttgcgatg
mea002	Reverse Biobricking of {xis!}	cgttaGGATCCtcatgacttcgccttcttccc

Construction of {int!} Biobrick Part K112207
PCR mea005/mea006 on pAH57		(1102 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256			(EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112207
----------------------------------------
mea005	Forward Biobricking of {int!}	ccataGAATTCatgAGATCTatgggaagaaggcgaagtcatg
mea006	Reverse Biobricking of {int!}	cgttaGGATCCttatttgatttcaattttgtcccactccc

Construction of {<int!} Biobrick Part K112208
PCR mea007/mea006 on pAH57		(1099 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256			(EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112208
----------------------------------------
mea007	Forward Biobricking of {<int!}	ccataGAATTCatgAGATCTggaagaaggcgaagtcatgagc
mea006	Reverse Biobricking of {int!}	cgttaGGATCCttatttgatttcaattttgtcccactccc

Construction of {int>} Biobrick Part K112209
PCR mea005/mea008 on pAH57		(1099 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256			(EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112209
----------------------------------------
mea005	Forward Biobricking of {int!}	ccataGAATTCatgAGATCTatgggaagaaggcgaagtcatg
mea008	Reverse Biobricking of {int>}	cgttaGGATCCtttgatttcaattttgtcccactccc

Construction of {<int>} Biobrick Part K112210
PCR mea007/mea008 on pAH57		(1096 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256			(EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112210
----------------------------------------
mea007	Forward Biobricking of {<int!}	ccataGAATTCatgAGATCTggaagaaggcgaagtcatgagc
mea008	Reverse Biobricking of {int>}	cgttaGGATCCtttgatttcaattttgtcccactccc

Construction of {a~int!} Biobrick Part K112211
PCR mea019/mea006 on pAH57		(1106 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256			(EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112211
----------------------------------------
mea019	Forward Biobricking of {a~int!}ccataGAATTCatgAGATCTagaaatgggaagaaggcgaag
mea006	Reverse Biobricking of {int!}	cgttaGGATCCttatttgatttcaattttgtcccactccc

Construction of {rbs.int!} Biobrick Part K112212
PCR mea020/mea006 on pAH57		(1120 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256			(EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112212
----------------------------------------
mea020	Forward Biobricking of {rbs.int!}ccataGAATTCatgAGATCTttttgaagaggatcagaaatggg
mea006	Reverse Biobricking of {int!}	cgttaGGATCCttatttgatttcaattttgtcccactccc

Construction of {ihfA!} Biobrick Part K112213
PCR mea009/mea010 on MG1655		(331 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256			(EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112213
----------------------------------------
mea009	Forward Biobricking of {ihfA!}ccataGAATTCatgAGATCTatggcgcttacaaaagctgaaatg
mea010	Reverse Biobricking of {ihfA!}	cgttaGGATCCttactcgtctttgggcgaagcg

Construction of {<ihfA!} Biobrick Part K112214
PCR mea011/mea010 on MG1655		(328 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256			(EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112214
----------------------------------------
mea011	Forward Biobricking of {<ihfA!}ccataGAATTCatgAGATCTgcgcttacaaaagctgaaatgtc
mea010	Reverse Biobricking of {ihfA!}	cgttaGGATCCttactcgtctttgggcgaagcg

Construction of {ihfA>} Biobrick Part K112215
PCR mea009/mea012 on MG1655		(328 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256			(EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112215
----------------------------------------
mea009	Forward Biobricking of {ihfA!}ccataGAATTCatgAGATCTatggcgcttacaaaagctgaaatg
mea012	Reverse Biobricking of {ihfA>}	cgttaGGATCCctcgtctttgggcgaagcg

Construction of {<ihfA>} Biobrick Part K112216
PCR mea011/mea012 on MG1655		(325 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256			(EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112216
----------------------------------------
mea011	Forward Biobricking of {<ihfA!}ccataGAATTCatgAGATCTgcgcttacaaaagctgaaatgtc
mea012	Reverse Biobricking of {ihfA>}	cgttaGGATCCctcgtctttgggcgaagcg

Construction of {a~ihfA!} Biobrick Part K112217
PCR mea021/mea010 on MG1655		(335 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256			(EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112217
----------------------------------------
mea021	Forward Biobricking of {a~ihfA!}ccataGAATTCatgAGATCTacctatggcgcttacaaaagc
mea010	Reverse Biobricking of {ihfA!}	cgttaGGATCCttactcgtctttgggcgaagcg

Construction of {rbs.ihfA!} Biobrick Part K112218
PCR mea022/mea010 on MG1655		(350 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256			(EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112218
----------------------------------------
mea022	Forward Biobricking of {rbs.ihfA!}ccataGAATTCatgAGATCTtcattgagggattgaacctatggcgc
mea010	Reverse Biobricking of {ihfA!}	cgttaGGATCCttactcgtctttgggcgaagcg

Construction of {ihfB!} Biobrick Part K112219
PCR mea013/mea014 on MG1655		(316 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256			(EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112219
----------------------------------------
mea013	Forward Biobricking of {ihfB!} ccataGAATTCatgAGATCTatgaccaagtcagaattgatagaaagacttgccaccc
mea014	Reverse Biobricking of {ihfB!}	cgttaGGATCCttaaccgtaaatattggcgcgatcg

Construction of {<ihfB!} Biobrick Part K112220
PCR mea015/mea014 on MG1655		(313 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256			(EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112220
----------------------------------------
mea015	Forward Biobricking of {<ihfB!} ccataGAATTCatgAGATCTaccaagtcagaattgatagaaagacttgccaccc
mea014	Reverse Biobricking of {ihfB!}	cgttaGGATCCttaaccgtaaatattggcgcgatcg

Construction of {ihfB>} Biobrick Part K112221
PCR mea013/mea016 on MG1655		(313 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256			(EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112221
----------------------------------------
mea013	Forward Biobricking of {ihfB!} ccataGAATTCatgAGATCTatgaccaagtcagaattgatagaaagacttgccaccc
mea016	Reverse Biobricking of {ihfB>}	cgttaGGATCCaccgtaaatattggcgcgatc

Construction of {<ihfB>} Biobrick Part K112222
PCR mea015/mea016 on MG1655		(310 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256			(EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112222
----------------------------------------
mea015	Forward Biobricking of {<ihfB!} ccataGAATTCatgAGATCTaccaagtcagaattgatagaaagacttgccaccc
mea016	Reverse Biobricking of {ihfB>}	cgttaGGATCCaccgtaaatattggcgcgatc

Construction of {a~ihfB!} Biobrick Part K112223
PCR mea023/mea014 on MG1655		(320 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256			(EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112223
----------------------------------------
mea023	Forward Biobricking of {a~ihfB!}ccataGAATTCatgAGATCTaatcatgaccaagtcagaattgatag
mea014	Reverse Biobricking of {ihfB!}	cgttaGGATCCttaaccgtaaatattggcgcgatcg

Construction of {rbs.ihfB!} Biobrick Part K112224
PCR mea024/mea014 on MG1655		(337 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256			(EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112224
----------------------------------------
mea024	Forward Biobricking of {rbs.ihfB!}ccataGAATTCatgAGATCTctttaaggaaccggaggaatcatg
mea014	Reverse Biobricking of {ihfB!}	cgttaGGATCCttaaccgtaaatattggcgcgatcg

Construction of {ompT>} Biobrick Part K112225
Wobble PCR mea025/mea026 		(97 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256			(EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112225
----------------------------------------
mea025	Forward Biobricking of {ompT>}	cgataGAATTCatgAGATCTatgcgggcgaaactcctaggaatagtcctgacaacccctatcg
mea026	Reverse Biobricking of {ompT>}	cgttaGGATCCcgtcgacgcaaaagagctgatcgcgataggggttgtcaggactattc

Construction of {a~ompT} Biobrick Part K112226
Wobble PCR mea027/mea026 		(101 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256			(EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112226
----------------------------------------
mea027	Forward Biobricking of {a~ompT}	cgataGAATTCatgAGATCTacatatgcgggcgaaactcctaggaatagtcctgacaacccctatc
mea026	Reverse Biobricking of {ompT>}	cgttaGGATCCcgtcgacgcaaaagagctgatcgcgataggggttgtcaggactattc

Construction of {rbs.ompT} Biobrick Part K112227
Wobble PCR mea028/mea026 		(114 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256			(EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112227
----------------------------------------
mea028	Forward Biobricking of {rbs.ompT}cgataGAATTCatgAGATCTaagaaggagatatacatatgcgggcgaaactcctaggaatagtcctgacaacccc
mea026	Reverse Biobricking of {ompT>}	cgttaGGATCCcgtcgacgcaaaagagctgatcgcgataggggttgtcaggactattc

Construction of {pelB>} Biobrick Part K112228
Wobble PCR mea029/mea030 		(97 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256			(EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112228
----------------------------------------
mea029	Forward Biobricking of {pelB>}	cgataGAATTCatgAGATCTatgaaatacctgctgccgaccgctgctgctggtctgctgctcc
mea030	Reverse Biobricking of {pelB>}	cgttaGGATCCggccatcgccggctgggcagcgaggagcagcagaccagcagcagcgg

Construction of {a~pelB} Biobrick Part K112229
Wobble PCR mea031/mea030 		(101 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256			(EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112229
----------------------------------------
mea031	Forward Biobricking of {a~pelB}	cgataGAATTCatgAGATCTacatatgaaatacctgctgccgaccgctgctgctggtctgctg
mea030	Reverse Biobricking of {pelB>}	cgttaGGATCCggccatcgccggctgggcagcgaggagcagcagaccagcagcagcgg

Construction of {rbs.pelB} Biobrick Part K112230
Wobble PCR mea032/mea030 		(116 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256			(EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112230
----------------------------------------
mea032	Forward Biobricking of {rbs.pelB} cgataGAATTCatgAGATCTttaagaaggagatatacatatgaaatacctgctgccgaccgctgctgctggtctgctg
mea030	Reverse Biobricking of {pelB>}	cgttaGGATCCggccatcgccggctgggcagcgaggagcagcagaccagcagcagcgg

Construction of {rbs.xis.int!} Biobrick Part K112234
PCR mea018/mea006 on pAH57 	        (1317 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256			(EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112234
----------------------------------------
mea018	Forward Biobricking of {rbs.xis!}ccagtGAATTCatgAGATCTtaattgcggagactttgcgatg
mea006	Reverse Biobricking of {int!}	cgttaGGATCCttatttgatttcaattttgtcccactccc


Molly's Notebook

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