Template:Team:UC Berkeley/Notebook/BX construction

From 2008.igem.org

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__TOC__
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==pBca1256-K112000==
<pre>
<pre>
PCR bx1/bx2 on Bjh1301 (688 bp, EcoRI/BamHI/DpnI)
PCR bx1/bx2 on Bjh1301 (688 bp, EcoRI/BamHI/DpnI)
-
Sub into pBca1256 (EcoRI/BamHI)
+
Sub into pBca1256 (EcoRI/BamHI)
Product is pBca1256-K112000
Product is pBca1256-K112000
----------------------------------------
----------------------------------------
bx1 Forward Biobricking of {T4 Holin!}
bx1 Forward Biobricking of {T4 Holin!}
-
CCATAGAATTCatgAGATCTatggcagcacctagaatatcat
+
ccagtGAATTCatgAGATCTatggcagcacctagaatatc
bx2 Reverse Biobricking of {T4 Holin!}
bx2 Reverse Biobricking of {T4 Holin!}
-
cgttaGGATCCttatttagcccttcctaatattct
+
cgttaGGATCCttatttagcccttcctaatattctggccgcc
</pre>
</pre>
 +
==pBca1256-K112001==
<pre>
<pre>
PCR bx3/bx2 on Bjh1301 (685 bp, EcoRI/BamHI/DpnI)
PCR bx3/bx2 on Bjh1301 (685 bp, EcoRI/BamHI/DpnI)
-
Sub into pBca1256 (EcoRI/BamHI)
+
Sub into pBca1256 (EcoRI/BamHI)
Product is pBca1256-K112001
Product is pBca1256-K112001
----------------------------------------
----------------------------------------
bx3 Forward biobricking of {<T4 Holin!}
bx3 Forward biobricking of {<T4 Holin!}
-
CCATAGAATTCatgAGATCTgcagcacctagaatatcattttcg
+
ccagtGAATTCatgAGATCTgcagcacctagaatatcattttcgccc
bx2 Reverse biobricking of {<T4 Holin!}
bx2 Reverse biobricking of {<T4 Holin!}
-
cgttaGGATCCttatttagcccttcctaatattc
+
cgttaGGATCCttatttagcccttcctaatattctggccgcc
</pre>
</pre>
 +
==pBca1256-K112002==
<pre>
<pre>
PCR bx1/bx4 on Bjh1301 (685 bp, EcoRI/BamHI/DpnI)
PCR bx1/bx4 on Bjh1301 (685 bp, EcoRI/BamHI/DpnI)
-
Sub into pBca1256 (EcoRI/BamHI)
+
Sub into pBca1256 (EcoRI/BamHI)
Product is pBca1256-K112002
Product is pBca1256-K112002
----------------------------------------
----------------------------------------
bx1 Forward biobricking of {T4 Holin>}
bx1 Forward biobricking of {T4 Holin>}
-
CCATAGAATTCatgAGATCTatggcagcacctagaatatcat
+
ccagtGAATTCatgAGATCTatggcagcacctagaatatc
bx4 Reverse biobricking of {T4 Holin>}
bx4 Reverse biobricking of {T4 Holin>}
cgttaGGATCCtttagcccttcctaatattctggcc
cgttaGGATCCtttagcccttcctaatattctggcc
</pre>
</pre>
 +
==pBca1256-K112003==
<pre>
<pre>
PCR bx3/bx4 on Bjh1301 (682 bp, EcoRI/BamHI/DpnI)
PCR bx3/bx4 on Bjh1301 (682 bp, EcoRI/BamHI/DpnI)
-
Sub into pBca1256 (EcoRI/BamHI)
+
Sub into pBca1256 (EcoRI/BamHI)
Product is pBca1256-K112003
Product is pBca1256-K112003
----------------------------------------
----------------------------------------
bx3 Forward Biobricking of {<T4 Holin>}
bx3 Forward Biobricking of {<T4 Holin>}
-
CCATAGAATTCatgAGATCTgcagcacctagaatatcattttcg
+
ccagtGAATTCatgAGATCTgcagcacctagaatatcattttcgccc
bx4 Reverse Biobricking of {<T4 Holin>}
bx4 Reverse Biobricking of {<T4 Holin>}
cgttaGGATCCtttagcccttcctaatattctggcc
cgttaGGATCCtttagcccttcctaatattctggcc
</pre>
</pre>
 +
==pBca1256-K112004==
<pre>
<pre>
-
PCR bx3/bx4 on Bjh1301 (692 bp, EcoRI/BamHI/DpnI)
+
PCR bx5/bx2 on Bjh1301 (692 bp, EcoRI/BamHI/DpnI)
-
Sub into pBca1256 (EcoRI/BamHI)
+
Sub into pBca1256 (EcoRI/BamHI)
Product is pBca1256-K112004
Product is pBca1256-K112004
----------------------------------------
----------------------------------------
bx5 Forward Biobricking of {a~T4 Holin!}
bx5 Forward Biobricking of {a~T4 Holin!}
-
CCATAGAATTCatgAGATCTgtctatggcagcacctagaat
+
ccagtGAATTCatgAGATCTgtctatggcagcacctagaatatc
bx2 Reverse Biobricking of {a~T4 Holin!}
bx2 Reverse Biobricking of {a~T4 Holin!}
-
cgttaGGATCCttatttagcccttcctaatattct
+
cgttaGGATCCttatttagcccttcctaatattctggccgcc
</pre>
</pre>
 +
==pBca1256-K112005==
<pre>
<pre>
PCR bx6/bx2 on Bjh1301 (704 bp, EcoRI/BamHI/DpnI)
PCR bx6/bx2 on Bjh1301 (704 bp, EcoRI/BamHI/DpnI)
-
Sub into pBca1256 (EcoRI/BamHI)
+
Sub into pBca1256 (EcoRI/BamHI)
Product is pBca1256-K112005
Product is pBca1256-K112005
----------------------------------------
----------------------------------------
bx6 Forward Biobricking of {rbs.T4 Holin!}
bx6 Forward Biobricking of {rbs.T4 Holin!}
-
CCATAGAATTCatgAGATCTcttaaaaggagggtctatggcagcacctagaatatc
+
ccagtGAATTCatgAGATCTcttaaaaggagggtctatggcagcacctagaatatc
bx2 Reverse Biobricking of {rbs.T4 Holin!}
bx2 Reverse Biobricking of {rbs.T4 Holin!}
-
cgttaGGATCCttatttagcccttcctaatattct
+
cgttaGGATCCttatttagcccttcctaatattctggccgcc
</pre>
</pre>
 +
==pBca1256-K112006==
<pre>
<pre>
PCR bx7/bx8 on Bjh1303 (325 bp, EcoRI/BamHI/DpnI)
PCR bx7/bx8 on Bjh1303 (325 bp, EcoRI/BamHI/DpnI)
-
Sub into pBca1256 (EcoRI/BamHI)
+
Sub into pBca1256 (EcoRI/BamHI)
Product is pBca1256-K112006
Product is pBca1256-K112006
----------------------------------------
----------------------------------------
bx7 Forward biobricking of {T4 Antiholin!}
bx7 Forward biobricking of {T4 Antiholin!}
-
CCATAGAATTCatgAGATCTatggccttaaaagcaacagcac
+
ccagtGAATTCatgAGATCTatggccttaaaagcaacagcac
bx8 Reverse biobricking of {T4 Antiholin!}
bx8 Reverse biobricking of {T4 Antiholin!}
-
cgttaGGATCCtcattcagtctccaatttaatg
+
cgttaGGATCCtcattcagtctccaatttaatgttc
</pre>
</pre>
 +
==pBca1256-K112007==
<pre>
<pre>
PCR bx9/bx8 on Bjh1303 (322 bp, EcoRI/BamHI/DpnI)
PCR bx9/bx8 on Bjh1303 (322 bp, EcoRI/BamHI/DpnI)
-
Sub into pBca1256 (EcoRI/BamHI)
+
Sub into pBca1256 (EcoRI/BamHI)
Product is pBca1256-K112007
Product is pBca1256-K112007
----------------------------------------
----------------------------------------
bx9 Forward biobricking of {<T4 Antiholin!}
bx9 Forward biobricking of {<T4 Antiholin!}
-
CCATAGAATTCatgAGATCTgccttaaaagcaacagcactt
+
ccagtGAATTCatgAGATCTgccttaaaagcaacagcac
bx8 Reverse biobricking of {<T4 Antiholin!}
bx8 Reverse biobricking of {<T4 Antiholin!}
-
cgttaGGATCCtcattcagtctccaatttaatg
+
cgttaGGATCCtcattcagtctccaatttaatgttc
</pre>
</pre>
 +
==pBca1256-K112008==
<pre>
<pre>
PCR bx7/bx10 on Bjh1303 (322 bp, EcoRI/BamHI/DpnI)
PCR bx7/bx10 on Bjh1303 (322 bp, EcoRI/BamHI/DpnI)
-
Sub into pBca1256 (EcoRI/BamHI)
+
Sub into pBca1256 (EcoRI/BamHI)
Product is pBca1256-K112008
Product is pBca1256-K112008
----------------------------------------
----------------------------------------
bx7 Forward biobricking of {T4 Antiholin>}
bx7 Forward biobricking of {T4 Antiholin>}
-
CCATAGAATTCatgAGATCTatggccttaaaagcaacagcac
+
ccagtGAATTCatgAGATCTatggccttaaaagcaacagcac
-
bx10 Reverse biobricking of {T4 Antiholin>}
+
bx10 Reverse biobricking of {T4 Antiholin>}
cgttaGGATCCttcagtctccaatttaatgttc
cgttaGGATCCttcagtctccaatttaatgttc
</pre>
</pre>
 +
==pBca1256-K112009==
<pre>
<pre>
PCR bx9/bx10 on Bjh1303 (319 bp, EcoRI/BamHI/DpnI)
PCR bx9/bx10 on Bjh1303 (319 bp, EcoRI/BamHI/DpnI)
-
Sub into pBca1256 (EcoRI/BamHI)
+
Sub into pBca1256 (EcoRI/BamHI)
Product is pBca1256-K112009
Product is pBca1256-K112009
----------------------------------------
----------------------------------------
bx9 Forward biobricking of {<T4 Antiholin>}
bx9 Forward biobricking of {<T4 Antiholin>}
-
CCATAGAATTCatgAGATCTgccttaaaagcaacagcactt
+
ccagtGAATTCatgAGATCTgccttaaaagcaacagcac
-
bx10 Reverse biobricking of {<T4 Antiholin>}
+
bx10 Reverse biobricking of {<T4 Antiholin>}
cgttaGGATCCttcagtctccaatttaatgttc
cgttaGGATCCttcagtctccaatttaatgttc
</pre>
</pre>
 +
==pBca1256-K112010==
<pre>
<pre>
-
PCR bx9/bx10 on Bjh1303 (329 bp, EcoRI/BamHI/DpnI)
+
PCR bx11/bx8 on Bjh1303 (329 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256 (EcoRI/BamHI)
Sub into pBca1256 (EcoRI/BamHI)
Product is pBca1256-K112010
Product is pBca1256-K112010
----------------------------------------
----------------------------------------
-
bx11 Forward biobricking of {a~T4 Antiholin!}
+
bx11 Forward biobricking of {a~T4 Antiholin!}
-
CCATAGAATTCatgAGATCTctttatggccttaaaagcaacagc
+
ccagtGAATTCatgAGATCTctttatggccttaaaagcaacagc
bx8 Reverse biobricking of {a~T4 Antiholin!}
bx8 Reverse biobricking of {a~T4 Antiholin!}
-
cgttaGGATCCtcattcagtctccaatttaatg
+
cgttaGGATCCtcattcagtctccaatttaatgttc
</pre>
</pre>
 +
==pBca1256-K112011==
<pre>
<pre>
PCR bx12/bx8 on Bjh1303 (339 bp, EcoRI/BamHI/DpnI)
PCR bx12/bx8 on Bjh1303 (339 bp, EcoRI/BamHI/DpnI)
-
Sub into pBca1256 (EcoRI/BamHI)
+
Sub into pBca1256 (EcoRI/BamHI)
Product is pBca1256-K112011
Product is pBca1256-K112011
----------------------------------------
----------------------------------------
bx12 Forward biobricking of {rbs.T4 Antiholin!}
bx12 Forward biobricking of {rbs.T4 Antiholin!}
-
CCATAGAATTCatgAGATCTgataggaggcctttatggccttaaaagcaacagc
+
ccagtGAATTCatgAGATCTgataggaggcctttatggccttaaaagcaacagc
bx8 Reverse biobricking of {rbs.T4 Antiholin!}
bx8 Reverse biobricking of {rbs.T4 Antiholin!}
-
cgttaGGATCCtcattcagtctccaatttaatg
+
cgttaGGATCCtcattcagtctccaatttaatgttc
</pre>
</pre>
 +
==pBca1256-K112012==
<pre>
<pre>
PCR bx13/bx14 on Bjh1302 (526 bp, EcoRI/BamHI/DpnI)
PCR bx13/bx14 on Bjh1302 (526 bp, EcoRI/BamHI/DpnI)
-
Sub into pBca1256 (EcoRI/BamHI)
+
Sub into pBca1256 (EcoRI/BamHI)
Product is pBca1256-K112012
Product is pBca1256-K112012
----------------------------------------
----------------------------------------
bx13      Forward biobricking of {T4 Endolysin!}
bx13      Forward biobricking of {T4 Endolysin!}
-
CCATAGAATTCatgAGATCTatgaatatatttgaaatgttacg
+
ccagtGAATTCatgAGATCTatgaatatatttgaaatgttacg
bx14      Reverse biobricking of {T4 Endolysin!}
bx14      Reverse biobricking of {T4 Endolysin!}
-
cgttaGGATCCttatagatttttatacgcgtccc
+
cgttaGGATCCttatagatttttatacgcgtcccaagtgcc
</pre>
</pre>
 +
==pBca1256-K112013==
<pre>
<pre>
PCR bx15/bx14 on Bjh1302 (523 bp, EcoRI/BamHI/DpnI)
PCR bx15/bx14 on Bjh1302 (523 bp, EcoRI/BamHI/DpnI)
-
Sub into pBca1256 (EcoRI/BamHI)
+
Sub into pBca1256 (EcoRI/BamHI)
Product is pBca1256-K112013
Product is pBca1256-K112013
----------------------------------------
----------------------------------------
bx15      Forward biobricking of {<T4 Endolysin!}
bx15      Forward biobricking of {<T4 Endolysin!}
-
CCATAGAATTCatgAGATCTaatatatttgaaatgttacgtatag
+
ccagtGAATTCatgAGATCTaatatatttgaaatgttacgtatagatg
bx14      Reverse biobricking of {<T4 Endolysin!}
bx14      Reverse biobricking of {<T4 Endolysin!}
-
cgttaGGATCCttatagatttttatacgcgtccc
+
cgttaGGATCCttatagatttttatacgcgtcccaagtgcc
</pre>
</pre>
 +
==pBca1256-K112014==
<pre>
<pre>
PCR bx13/bx16 on Bjh1302 (523 bp, EcoRI/BamHI/DpnI)
PCR bx13/bx16 on Bjh1302 (523 bp, EcoRI/BamHI/DpnI)
-
Sub into pBca1256 (EcoRI/BamHI)
+
Sub into pBca1256 (EcoRI/BamHI)
Product is pBca1256-K112014
Product is pBca1256-K112014
----------------------------------------
----------------------------------------
bx13      Forward biobricking of {T4 Endolysin>}
bx13      Forward biobricking of {T4 Endolysin>}
-
CCATAGAATTCatgAGATCTatgaatatatttgaaatgttacg
+
ccagtGAATTCatgAGATCTatgaatatatttgaaatgttacg
bx16      Reverse biobricking of {T4 Endolysin>}
bx16      Reverse biobricking of {T4 Endolysin>}
-
cgttaGGATCCtagatttttatacgcgtcccaagtg
+
cgttaGGATCCtagatttttatacgcgtcccaagtgcc
</pre>
</pre>
 +
==pBca1256-K112015==
<pre>
<pre>
PCR bx15/bx16 on Bjh1302 (520 bp, EcoRI/BamHI/DpnI)
PCR bx15/bx16 on Bjh1302 (520 bp, EcoRI/BamHI/DpnI)
-
Sub into pBca1256 (EcoRI/BamHI)
+
Sub into pBca1256 (EcoRI/BamHI)
Product is pBca1256-K112015
Product is pBca1256-K112015
----------------------------------------
----------------------------------------
bx15      Forward biobricking of {<T4 Endolysin>}
bx15      Forward biobricking of {<T4 Endolysin>}
-
CCATAGAATTCatgAGATCTaatatatttgaaatgttacgtatag
+
ccagtGAATTCatgAGATCTaatatatttgaaatgttacgtatagatg
bx16      Reverse biobricking of {<T4 Endolysin>}
bx16      Reverse biobricking of {<T4 Endolysin>}
-
cgttaGGATCCtagatttttatacgcgtcccaagtg
+
cgttaGGATCCtagatttttatacgcgtcccaagtgcc
</pre>
</pre>
 +
==pBca1256-K112016==
<pre>
<pre>
PCR bx17/bx16 on Bjh1302 (530 bp, EcoRI/BamHI/DpnI)
PCR bx17/bx16 on Bjh1302 (530 bp, EcoRI/BamHI/DpnI)
-
Sub into pBca1256 (EcoRI/BamHI)
+
Sub into pBca1256 (EcoRI/BamHI)
Product is pBca1256-K112016
Product is pBca1256-K112016
----------------------------------------
----------------------------------------
bx17      Forward biobricking of {a~T4 Endolysin!}
bx17      Forward biobricking of {a~T4 Endolysin!}
-
CCATAGAATTCatgAGATCTtattatgaatatatttgaaatg
+
ccagtGAATTCatgAGATCTtattatgaatatatttgaaatgttacgtatagatg
bx16      Reverse biobricking of {a~T4 Endolysin!}
bx16      Reverse biobricking of {a~T4 Endolysin!}
-
cgttaGGATCCtagatttttatacgcgtcccaagtg
+
cgttaGGATCCtagatttttatacgcgtcccaagtgcc
</pre>
</pre>
 +
==pBca1256-K112017==
<pre>
<pre>
PCR bx18/bx16 on Bjh1302 (542 bp, EcoRI/BamHI/DpnI)
PCR bx18/bx16 on Bjh1302 (542 bp, EcoRI/BamHI/DpnI)
-
Sub into pBca1256 (EcoRI/BamHI)
+
Sub into pBca1256 (EcoRI/BamHI)
Product is pBca1256-K112017
Product is pBca1256-K112017
----------------------------------------
----------------------------------------
bx18      Forward biobricking of {rbs.T4 Endolysin!}
bx18      Forward biobricking of {rbs.T4 Endolysin!}
-
CCATAGAATTCatgAGATCTatacttaggaggtattatgaatatatttgaaatgttac
+
ccagtGAATTCatgAGATCTatacttaggaggtattatgaatatatttgaaatgttac
bx16      Reverse biobricking of {rbs.T4 Endolysin!}
bx16      Reverse biobricking of {rbs.T4 Endolysin!}
-
cgttaGGATCCtagatttttatacgcgtcccaagtg
+
cgttaGGATCCtagatttttatacgcgtcccaagtgcc
</pre>
</pre>
Line 202: Line 222:
----
----
<div style="text-align: center;"> [[Team:UC_Berkeley/Notebook/Bing_Xia|Bing Xia]] </div>
<div style="text-align: center;"> [[Team:UC_Berkeley/Notebook/Bing_Xia|Bing Xia]] </div>
 +
<div style="text-align: center;"> [[Team:UC_Berkeley|Back to Berkeley]] </div>

Latest revision as of 01:36, 12 June 2008

Contents


pBca1256-K112000

PCR bx1/bx2 on Bjh1301		(688 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256			(EcoRI/BamHI)
Product is pBca1256-K112000
----------------------------------------
bx1		Forward Biobricking of {T4 Holin!}
ccagtGAATTCatgAGATCTatggcagcacctagaatatc
bx2		Reverse Biobricking of {T4 Holin!}
cgttaGGATCCttatttagcccttcctaatattctggccgcc

pBca1256-K112001

PCR bx3/bx2 on Bjh1301		(685 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256			(EcoRI/BamHI)
Product is pBca1256-K112001
----------------------------------------
bx3		Forward biobricking of {<T4 Holin!}
ccagtGAATTCatgAGATCTgcagcacctagaatatcattttcgccc
bx2		Reverse biobricking of {<T4 Holin!}
cgttaGGATCCttatttagcccttcctaatattctggccgcc

pBca1256-K112002

PCR bx1/bx4 on Bjh1301		(685 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256			(EcoRI/BamHI)
Product is pBca1256-K112002
----------------------------------------
bx1		Forward biobricking of {T4 Holin>}
ccagtGAATTCatgAGATCTatggcagcacctagaatatc
bx4		Reverse biobricking of {T4 Holin>}
cgttaGGATCCtttagcccttcctaatattctggcc

pBca1256-K112003

PCR bx3/bx4 on Bjh1301		(682 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256			(EcoRI/BamHI)
Product is pBca1256-K112003
----------------------------------------
bx3		Forward Biobricking of {<T4 Holin>}
ccagtGAATTCatgAGATCTgcagcacctagaatatcattttcgccc
bx4		Reverse Biobricking of {<T4 Holin>}
cgttaGGATCCtttagcccttcctaatattctggcc

pBca1256-K112004

PCR bx5/bx2 on Bjh1301		(692 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256			(EcoRI/BamHI)
Product is pBca1256-K112004
----------------------------------------
bx5		Forward Biobricking of {a~T4 Holin!}
ccagtGAATTCatgAGATCTgtctatggcagcacctagaatatc
bx2		Reverse Biobricking of {a~T4 Holin!}
cgttaGGATCCttatttagcccttcctaatattctggccgcc

pBca1256-K112005

PCR bx6/bx2 on Bjh1301		(704 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256			(EcoRI/BamHI)
Product is pBca1256-K112005
----------------------------------------
bx6		Forward Biobricking of {rbs.T4 Holin!}
ccagtGAATTCatgAGATCTcttaaaaggagggtctatggcagcacctagaatatc
bx2		Reverse Biobricking of {rbs.T4 Holin!}
cgttaGGATCCttatttagcccttcctaatattctggccgcc

pBca1256-K112006

PCR bx7/bx8 on Bjh1303		(325 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256			(EcoRI/BamHI)
Product is pBca1256-K112006
----------------------------------------
bx7		Forward biobricking of {T4 Antiholin!}
ccagtGAATTCatgAGATCTatggccttaaaagcaacagcac
bx8		Reverse biobricking of {T4 Antiholin!}
cgttaGGATCCtcattcagtctccaatttaatgttc

pBca1256-K112007

PCR bx9/bx8 on Bjh1303		(322 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256			(EcoRI/BamHI)
Product is pBca1256-K112007
----------------------------------------
bx9		Forward biobricking of {<T4 Antiholin!}
ccagtGAATTCatgAGATCTgccttaaaagcaacagcac
bx8		Reverse biobricking of {<T4 Antiholin!}
cgttaGGATCCtcattcagtctccaatttaatgttc

pBca1256-K112008

PCR bx7/bx10 on Bjh1303		(322 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256			(EcoRI/BamHI)
Product is pBca1256-K112008
----------------------------------------
bx7		Forward biobricking of {T4 Antiholin>}
ccagtGAATTCatgAGATCTatggccttaaaagcaacagcac
bx10	Reverse biobricking of {T4 Antiholin>}
cgttaGGATCCttcagtctccaatttaatgttc

pBca1256-K112009

PCR bx9/bx10 on Bjh1303		(319 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256			(EcoRI/BamHI)
Product is pBca1256-K112009
----------------------------------------
bx9		Forward biobricking of {<T4 Antiholin>}
ccagtGAATTCatgAGATCTgccttaaaagcaacagcac
bx10	Reverse biobricking of {<T4 Antiholin>}
cgttaGGATCCttcagtctccaatttaatgttc

pBca1256-K112010

PCR bx11/bx8 on Bjh1303		(329 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256			(EcoRI/BamHI)
Product is pBca1256-K112010
----------------------------------------
bx11	Forward biobricking of {a~T4 Antiholin!}
ccagtGAATTCatgAGATCTctttatggccttaaaagcaacagc
bx8		Reverse biobricking of {a~T4 Antiholin!}
cgttaGGATCCtcattcagtctccaatttaatgttc

pBca1256-K112011

PCR bx12/bx8 on Bjh1303		(339 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256			(EcoRI/BamHI)
Product is pBca1256-K112011
----------------------------------------
bx12	Forward biobricking of {rbs.T4 Antiholin!}
ccagtGAATTCatgAGATCTgataggaggcctttatggccttaaaagcaacagc
bx8		Reverse biobricking of {rbs.T4 Antiholin!}
cgttaGGATCCtcattcagtctccaatttaatgttc

pBca1256-K112012

PCR bx13/bx14 on Bjh1302		(526 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256				(EcoRI/BamHI)
Product is pBca1256-K112012
----------------------------------------
bx13      Forward biobricking of {T4 Endolysin!}
ccagtGAATTCatgAGATCTatgaatatatttgaaatgttacg
bx14      Reverse biobricking of {T4 Endolysin!}
cgttaGGATCCttatagatttttatacgcgtcccaagtgcc

pBca1256-K112013

PCR bx15/bx14 on Bjh1302		(523 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256				(EcoRI/BamHI)
Product is pBca1256-K112013
----------------------------------------
bx15      Forward biobricking of {<T4 Endolysin!}
ccagtGAATTCatgAGATCTaatatatttgaaatgttacgtatagatg
bx14      Reverse biobricking of {<T4 Endolysin!}
cgttaGGATCCttatagatttttatacgcgtcccaagtgcc

pBca1256-K112014

PCR bx13/bx16 on Bjh1302		(523 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256				(EcoRI/BamHI)
Product is pBca1256-K112014
----------------------------------------
bx13      Forward biobricking of {T4 Endolysin>}
ccagtGAATTCatgAGATCTatgaatatatttgaaatgttacg
bx16      Reverse biobricking of {T4 Endolysin>}
cgttaGGATCCtagatttttatacgcgtcccaagtgcc

pBca1256-K112015

PCR bx15/bx16 on Bjh1302		(520 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256				(EcoRI/BamHI)
Product is pBca1256-K112015
----------------------------------------
bx15      Forward biobricking of {<T4 Endolysin>}
ccagtGAATTCatgAGATCTaatatatttgaaatgttacgtatagatg
bx16      Reverse biobricking of {<T4 Endolysin>}
cgttaGGATCCtagatttttatacgcgtcccaagtgcc

pBca1256-K112016

PCR bx17/bx16 on Bjh1302		(530 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256				(EcoRI/BamHI)
Product is pBca1256-K112016
----------------------------------------
bx17      Forward biobricking of {a~T4 Endolysin!}
ccagtGAATTCatgAGATCTtattatgaatatatttgaaatgttacgtatagatg
bx16      Reverse biobricking of {a~T4 Endolysin!}
cgttaGGATCCtagatttttatacgcgtcccaagtgcc

pBca1256-K112017

PCR bx18/bx16 on Bjh1302		(542 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256				(EcoRI/BamHI)
Product is pBca1256-K112017
----------------------------------------
bx18      Forward biobricking of {rbs.T4 Endolysin!}
ccagtGAATTCatgAGATCTatacttaggaggtattatgaatatatttgaaatgttac
bx16      Reverse biobricking of {rbs.T4 Endolysin!}
cgttaGGATCCtagatttttatacgcgtcccaagtgcc



Bing Xia
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