Imperial College/7 September 2008
From 2008.igem.org
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+ | ==Dry Lab: Motility Analysis== | ||
+ | =====Monday===== | ||
+ | *Track 20 cells | ||
+ | *Determine the segmentation of the data: | ||
+ | **plotting velocity to exp(i*theta) | ||
+ | **where theta is the difference between the angle of rotation of the cell's tip to its centroid and the angle of the centroid displacement | ||
+ | |||
+ | =====Tuesday===== | ||
+ | *Track 20 cells | ||
+ | *Prepare for meeting with supervisors | ||
+ | |||
+ | =====Wednesday===== | ||
+ | *Acquire longer microscope videos of swimming B. Subtilis | ||
+ | *Track at least 20 cells manually | ||
+ | *Adjust matlab code according to the segmentation of the data | ||
+ | |||
+ | =====Thursday===== | ||
+ | |||
+ | *Hopefully extract run_time, tumble_time,run velocity and tumble angle using our matlab code | ||
+ | *Plot their distributions and compare to model | ||
+ | |||
+ | =====Friday===== | ||
+ | *Acquire more data by tracking cells manually | ||
+ | *In theory we would need to track about 100 cells to 150 cells | ||
+ | |||
==Wetlab== | ==Wetlab== | ||
===''B.subtilis''=== | ===''B.subtilis''=== | ||
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*Finish any unfinished jobs from earlier in week | *Finish any unfinished jobs from earlier in week | ||
- | {{Imperial/EndPage}} | + | <br> |
+ | {{Imperial/EndPage|Notebook|Notebook}} |
Latest revision as of 20:45, 28 October 2008
7 September 2008Dry Lab: Motility AnalysisMonday
Tuesday
Wednesday
Thursday
Friday
WetlabB.subtilisMonday
Tuesday
Wednesday
Thursday
Friday
CloningOngoing/Where there is spare time
Monday
Tuesday
Wednesday
Thursday
Friday
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