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Rensselaer/8 September 2008
From 2008.igem.org
(Difference between revisions)
(New page: '''DNA purification digest''' Fe(13ug) Plasmid(11ug) DNA 280ul 95ul XbaI 10 10 SprI 20 20 Buffer2 40 17.5 BSA 4 1.75 dH2O 46 ...) |
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'''DNA purification digest''' | '''DNA purification digest''' | ||
| - | + | ||
| + | Fe(13ug) Plasmid(11ug) | ||
| + | |||
DNA 280ul 95ul | DNA 280ul 95ul | ||
| + | |||
XbaI 10 10 | XbaI 10 10 | ||
| + | |||
SprI 20 20 | SprI 20 20 | ||
| + | |||
Buffer2 40 17.5 | Buffer2 40 17.5 | ||
| + | |||
BSA 4 1.75 | BSA 4 1.75 | ||
| + | |||
dH2O 46 30.75 | dH2O 46 30.75 | ||
| - | + | ||
| - | + | ____ _____ | |
| + | |||
| + | 400 ul 175 ul | ||
'''Insert checks digest''' | '''Insert checks digest''' | ||
| - | + | ||
| + | Fe-mRFP (~1ug) | ||
| + | |||
DNA(30ug/ul) 33 | DNA(30ug/ul) 33 | ||
| + | |||
MsCI(3U/ul) 6.7 | MsCI(3U/ul) 6.7 | ||
| + | |||
EcoRI(2U/ul) 1 | EcoRI(2U/ul) 1 | ||
| + | |||
EcoBuff 5 | EcoBuff 5 | ||
| + | |||
BSA 0.5 | BSA 0.5 | ||
| + | |||
dH2O 3.8 | dH2O 3.8 | ||
| - | + | ||
| - | + | _____ | |
| + | |||
| + | 50 ul | ||
Latest revision as of 20:55, 8 September 2008
DNA purification digest
Fe(13ug) Plasmid(11ug)
DNA 280ul 95ul
XbaI 10 10
SprI 20 20
Buffer2 40 17.5
BSA 4 1.75
dH2O 46 30.75
____ _____
400 ul 175 ul
Insert checks digest
Fe-mRFP (~1ug)
DNA(30ug/ul) 33
MsCI(3U/ul) 6.7
EcoRI(2U/ul) 1
EcoBuff 5
BSA 0.5
dH2O 3.8
_____
50 ul
