Team:University of Ottawa/5 June 2008

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__TOC__
==Today in the Lab==
==Today in the Lab==
'''Dan, Matt'''
'''Dan, Matt'''
-
:'''igestion'''
+
:'''Digestion'''
::<li>We decided to use different enzymes - Bam HI + EcoRI for 8/7 and xhoI for 0/1.
::<li>We decided to use different enzymes - Bam HI + EcoRI for 8/7 and xhoI for 0/1.
:'''Gel Results'''
:'''Gel Results'''
::<li>Gel was unsuccessful for 1A but results for other plasmids worked well.
::<li>Gel was unsuccessful for 1A but results for other plasmids worked well.
 +
:'''Miniprep'''
::<li>We decided to choose 6 new colonies for the 1A plasmid and perform a miniprep on them.
::<li>We decided to choose 6 new colonies for the 1A plasmid and perform a miniprep on them.
::<li>Colonies were then inoculated using LB + Amp solution and left overnight.
::<li>Colonies were then inoculated using LB + Amp solution and left overnight.
:'''Glycerol Stock'''
:'''Glycerol Stock'''
::<li>Glycerol Stock was performed for plasmids 7/8 and 0.
::<li>Glycerol Stock was performed for plasmids 7/8 and 0.

Latest revision as of 22:38, 28 October 2008

Untitled Document

 

 


Contents

Today in the Lab

Dan, Matt

Digestion
  • We decided to use different enzymes - Bam HI + EcoRI for 8/7 and xhoI for 0/1.
    • Gel Results
  • Gel was unsuccessful for 1A but results for other plasmids worked well.
    • Miniprep
  • We decided to choose 6 new colonies for the 1A plasmid and perform a miniprep on them.
  • Colonies were then inoculated using LB + Amp solution and left overnight.
    • Glycerol Stock
  • Glycerol Stock was performed for plasmids 7/8 and 0.
    • Retrieved from "http://2008.igem.org/Team:University_of_Ottawa/5_June_2008"