Template:Team:UC Berkeley/Notebook/AL construction

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Latest revision as of 02:10, 29 October 2008

1 pBca1256-K112300
2 pBca1256-K112301
3 pBca1256-K112302
4 pBca1256-K112303
5 pBca1256-K112304
6 pBca1256-K112305
7 pBca1256-K112306
8 pBca1256-K112307
9 pBca1256-K112308
10 pBca1256-K112309
11 pBca1256-K112310
12 pBca1256-K112311
13 pBca1256-K112312
14 pBca1256-K112313
15 pBca1256-K112314
16 pBca1256-K112315
17 pBca1256-K112316
18 pBca1256-K112317
19 pBca1256-K112318
20 pBca1256-K112319
21 pBca1256-K112320
22 pBjh1601AC-K112321
23 pBjh1601CK-K112322
24 pBCa1601AC-K112323
25 pBCa1601CK-K112324
26 pBCa1601CK-K112325
27 pBCa1601AC-K112327
28 pBCa1601AC-K112328
29 pBCa1601AC-K112329
30 pBCa1601AC-K112330
31 pBCa1601AC-K112331
32 pBCa1601AC-K112332
33 pBCa1601AC-K112333
34 pBca1601AK-K112334
35 pBca1601AK-K112335
36 pBca1601AK-K112336
37 pBca1601AK-K112337
38 pBca1601AK-K112339
39 pBca1601AK-K112340
40 pBca1601AK-K112341
41 pBca1601AK-K112342
42 pBca1601AK-K11234
43 pBca1601AK-K112348
44 pBca1601AK-K112349
45 pBca1601AK-K112350
46 pBca1601AK-K112351
47 pBca1601CK-K112354
48 pBca1601CK-K112355
49 pBca1601CK-K112356
50 pBca1601CK-K112357
51 pBca1601CK-K112360
52 pBca1601CK-K112361
53 pBca1601CK-K112362
54 pBca1601CK-K112363
55 pBca1601CK-K112368
56 pBca1601CK-K112369
57 pBca1601CK-K112370
58 pBca1601CK-K112371
59 pBca1601CK-K112372
60 pBca1601CK-K112373

pBca1256-K112300

PCR al0001/al0002 on pSB3C6-Bjh1501     (508bp, EcoRI/BamHI/DpnI)
Sub into pBca1256                      (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112300
-----------------------------------------------
al0001	Forward Biobricking of lambda lysozyme with start and stop codons
CCATAGAATTCatgAGATCTatggtagaaatcaataatcaacgtaaggcgttcctcg
al0002	Reverse Biobricking of lambda lysozyme with start and stop codons
CGttaGGATCCtcatacatcaatctctctgaccgttccgcc

pBca1256-K112301

PCR al0003/al0002 on pSB3C6-Bjh1501     (505bp, EcoRI/BamHI/DpnI)
Sub into pBca1256                      (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112301
-----------------------------------------------
al0003	Forward Biobricking of Lambda Lysozyme with stop codon and no start codon
CCATAGAATTCatgAGATCTgtagaaatcaataatcaacgtaaggcgttcctcg
al0002	Reverse Biobricking of Lambda Lysozyme with stop codon and no start codon
CGttaGGATCCtcatacatcaatctctctgaccgttccgcc

pBca1256-K112302

PCR al0001/al0004 on pSB3C6-Bjh1501     (505bp, EcoRI/BamHI/DpnI)
Sub into pBca1256                      (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112302
-----------------------------------------------
al0001	Forward Biobricking of lambda lysozyme with start codon and no stop codon
CCATAGAATTCatgAGATCTatggtagaaatcaataatcaacgtaaggcgttcctcg
al0004	Reverse Biobricking of lambda lysozyme with start codon and no stop codon
CGttaGGATCCtacatcaatctctctgaccgttccgc

pBca1256-K112303

PCR al0003/al0004 on pSB3C6-Bjh1501     (502bp, EcoRI/BamHI/DpnI)
Sub into pBca1256                      (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112303
-----------------------------------------------
al0003	Forward Biobricking of lambda lysozyme with no start/stop codon
CCATAGAATTCatgAGATCTgtagaaatcaataatcaacgtaaggcgttcctcg
al0004	Reverse Biobricking of lambda lysozyme with no start/stop codon
CGttaGGATCCtacatcaatctctctgaccgttccgc

pBca1256-K112304

PCR al0005/al0002 on pSB3C6-Bjh1501     (512bp, EcoRI/BamHI/DpnI)
Sub into pBca1256                      (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112304
-----------------------------------------------
al0005	Forward Biobricking of lambda lysozyme with spacer
CCATAGAATTCatgAGATCTgaagatggtagaaatcaataatcaacgtaaggcgttcctcg
al0002	Reverse Biobricking of lambda lysozyme with spacer
CGttaGGATCCtcatacatcaatctctctgaccgttccgcc

pBca1256-K112305

PCR al0006/al0002 on pSB3C6-Bjh1501     (527bp, EcoRI/BamHI/DpnI)
Sub into pBca1256                      (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112305
-----------------------------------------------
al0006	Forward Biobricking of lambda lysozyme with rbs site
CCATAGAATTCatgAGATCTaaaaaagccggagtagaagatgg
al0002	Reverse Biobricking of lambda lysozyme with rbs site
CGttaGGATCCtcatacatcaatctctctgaccgttccgcc

pBca1256-K112306

PCR al0007/al0008 on pSB3C6-Bjh1502     (349bp, EcoRI/BamHI/DpnI)
Sub into pBca1256                      (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112306
-----------------------------------------------
al0007	Forward Biobricking of lambda holin with start and stop codons
CCATAGAATTCatgAGATCTatgccagaaaaacatgacctgttgg
al0008	Reverse Biobricking of lambda holin with start and stop codons
CGttaGGATCCttattgatttctaccatcttctactccggc

pBca1256-K112307

PCR al0009/al0008 on pSB3C6-Bjh1502     (346bp, EcoRI/BamHI/DpnI)
Sub into pBca1256                      (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112307
-----------------------------------------------
al0009	Forward Biobricking of lambda holin with stop codon and no start codon
CCATAGAATTCatgAGATCTccagaaaaacatgacctgttggcc
al0008	Reverse Biobricking of lambda holin with stop codon and no start codon
CGttaGGATCCttattgatttctaccatcttctactccggc

pBca1256-K112308

PCR al0007/al0010 on pSB3C6-Bjh1502     (346bp, EcoRI/BamHI/DpnI)
Sub into pBca1256                      (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112308
-----------------------------------------------
al0007	Forward Biobricking of lambda holin with start and no stop codon
CCATAGAATTCatgAGATCTatgccagaaaaacatgacctgttgg
al0010	Reverse Biobricking of lambda holin wtih start and no stop codon
CGttaGGATCCttgatttctaccatcttctactccggc

pBca1256-K112309

PCR al0009/al0010 on pSB3C6-Bjh1502     (343bp, EcoRI/BamHI/DpnI)
Sub into pBca1256                      (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112309
-----------------------------------------------
al0009	Forward Biobricking of lambda holin with no start and stop codon
CCATAGAATTCatgAGATCTccagaaaaacatgacctgttggcc
al0010	Reverse Biobricking of lambda holin with no start and stop codon
CGttaGGATCCttgatttctaccatcttctactccggc

pBca1256-K112310

PCR al0011/al0008 on pSB3C6-Bjh1502     (353bp, EcoRI/BamHI/DpnI)
Sub into pBca1256                      (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112310
-----------------------------------------------
al0011	Forward Biobricking of lambda holin with spacer
CCATAGAATTCatgAGATCTgaagatgccagaaaaacatgacctg
al0008	Reverse Biobricking of lambda holin with spacer
CGttaGGATCCttattgatttctaccatcttctactccggc

pBca1256-K112311

PCR al0012/al0008 on pSB3C6-Bjh1502     (369bp, EcoRI/BamHI/DpnI)
Sub into pBca1256                      (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112311
-----------------------------------------------
al0012	Forward Biobricking of lambda holin with rbs site
CCATAGAATTCatgAGATCTattgggggtaagacCtgaag
al0008	Reverse Biobricking of lambda holin with rbs site
CGttaGGATCCttattgatttctaccatcttctactccggc

pBca1256-K112312

PCR al0013/al0008 on pSB3C6-Bjh1503     (355bp, EcoRI/BamHI/DpnI)
Sub into pBca1256                      (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112312
-----------------------------------------------
al0013	Forward Biobricking of lambda antiholin with start and stop codons
CCATAGAATTCatgAGATCTatgaagCtgccagaaaaacatgacc
al0008	Reverse Biobricking of lambda antiholin with start and stop codons
CGttaGGATCCttattgatttctaccatcttctactccggc

pBca1256-K112313

PCR al0014/al0008 on pSB3C6-Bjh1503     (352bp, EcoRI/BamHI/DpnI)
Sub into pBca1256                      (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112313
-----------------------------------------------
al0014	Forward Biobricking of lambda antiholin with stop codon and no start codon
CCATAGAATTCatgAGATCTaagCtgccagaaaaacatgacctg
al0008	Reverse Biobricking of lambda antiholin with stop codon and no start codon
CGttaGGATCCttattgatttctaccatcttctactccggc

pBca1256-K112314

PCR al0013/al0010 on pSB3C6-Bjh1503     (352bp, EcoRI/BamHI/DpnI)
Sub into pBca1256                      (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112314
-----------------------------------------------
al0013	Forward Biobricking of lambda antiholin with start codon and no stop codon
CCATAGAATTCatgAGATCTatgaagCtgccagaaaaacatgacc
al0010	Reverse Biobricking of lambda antiholin with start codon and no stop codon
CGttaGGATCCttgatttctaccatcttctactccggc

pBca1256-K112315

PCR al0014/al0010 on pSB3C6-Bjh1503     (349bp, EcoRI/BamHI/DpnI)
Sub into pBca1256                      (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112315
-----------------------------------------------
al0014	Forward Biobricking of lambda antiholin with no start and stop codons
CCATAGAATTCatgAGATCTaagCtgccagaaaaacatgacctg
al0010	Reverse Biobricking of lambda antiholin with no start and stop codons
CGttaGGATCCttgatttctaccatcttctactccggc

pBca1256-K112316

PCR al0015/al0008 on pSB3C6-Bjh1503     (359bp, EcoRI/BamHI/DpnI)
Sub into pBca1256                      (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112316
-----------------------------------------------
al0015	Forward Biobricking of lambda antiholin with spacer
CCATAGAATTCatgAGATCTagacatgaagCtgccagaaaaacatgacc
al0008	Reverse Biobricking of lambda antiholin with spacer
CGttaGGATCCttattgatttctaccatcttctactccggc

pBca1256-K112317

PCR al0016/al0008 on pSB3C6-Bjh1503     (375bp, EcoRI/BamHI/DpnI)
Sub into pBca1256                      (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112317
-----------------------------------------------
al0016	Forward Biobricking of lambda antiholin with rbs site
CCATAGAATTCatgAGATCTccccttattgggggtaagacatg
al0008	Reverse Biobricking of lambda antiholin with rbs site
CGttaGGATCCttattgatttctaccatcttctactccggc

pBca1256-K112318

PCR al0017/al0018 on MG1655     (467bp, EcoRI/BamHI/DpnI)
Sub into pBca1256                      (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112318
-----------------------------------------------
al0017	Forward Biobricking of bolA promoter
CCATAGAATTCatgAGATCTtgctgtggcagtgtaatcgtc
al0018	Reverse Biobricking of bolA promoter
CGttaGGATCCcttctatccgctcacgtatc

pBca1256-K112319

PCR al0019/al0024 on MG1655 (404bp, gp = A)
PCR al0023/al0023 on MG1655 (86bp, gp=B)
-----------------------------------------------
PCR al0019/al0020 on A+B     (465bp, EcoRI/BamHI/DpnI)
Sub into pBca1256                      (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112319
-----------------------------------------------
al0019	Forward Biobricking of ftsQ promoter
CCATAGAATTCatgAGATCTttgattgaaaaatggctaagtgggccgg
al0020	Reverse Biobricking of ftsQ promoter
CGttaGGATCCcctcttcttcgctgtttcgc
al0023 Forward Removing of EcoRI
ggtagtacgaattGtggaactggcg
al0024 Reverse Removing of EcoRI
cgccagttccaCaattcgtactacc

pBca1256-K112320

PCR al0021/al0022 on MG1655     (804bp, EcoRI/BamHI/DpnI)
Sub into pBca1256                      (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112320
-----------------------------------------------
al0021	Forward Biobricking of ftsAZ promoter
CCATAGAATTCatgAGATCTaacgacagtgttggtgagcg
al0022	Reverse Biobricking of ftsAZ promoter
CGttaGGATCCgtaccaatctccagtcctactaccag

pBjh1601AC-K112321

PCR JH088F/JHO88R on Salmonella L2.     (435bp, EcoRI/BamHI/DpnI)
Sub into pBjh1601AC                      (EcoRI/BamHI, 3195+910, L)
Product is pBjh1601AC-K112321
-----------------------------------------------
JH088F   Forward biobricking of {H-NS!}   
cgataGAATTCatgAGATCTATGAGCGAAGCACTTAAAATTC
JH088R   Reverse biobricking of {H-NS!}   
cgttaGGATCCTTATTGCTTGATCAGGAAATCG

pBjh1601CK-K112322

PCR JH089F/JH089R on Salmonella L2.    (379 bp, EcoRI/BamHI/DpnI)
Sub into pBjh1601CK             (EcoRI/BamHI, 3134+910, L)
Product is pBjh1601CK-K112322
-----------------------------------------------
JH089F   Forward biobricking of {Pdps}   
cgataGAATTCatgAGATCTaacgattcgacgcgaaccgc
JH089R   Reverse biobricking of {Pdps}   
cgttaGGATCCaatctcatatcctcttgatg

pBCa1601AC-K112323

PCR ca998/JH090R on pBjh1601AC-K112321-2    (445 bp, EcoRI/BamHI)
Sub into pBjh1601AC                      (EcoRI/BamHI, 3195+910, L)
Product is pBjh1601AC-K112323
-----------------------------------------------
ca998   Forward biobricking of {H-NS!} Salmonella   
gtatcacgaggcagaatttcag
JH090R   Reverse biobricking of {H-NS!} Salmonella   
cgttaGGATCCTTATTcCTTGATCAGG

pBCa1601CK-K112324

Digest (BgIII methylated) K112902 CA, (BamHI methylated) K112709 AK      (BamHI/BgIII/XHoI)
Product is pBjh1601CK-K112324

pBCa1601CK-K112325

Digest (BgIII methylated) K112704 CA, (BamHI methylated) K112709 AK      (BamHI/BgIII/XHoI)
Product is pBjh1601CK-K112325

pBCa1601AC-K112327

Digest (BgIII methylated) K112230 AK, (BamHI methylated) K112203 KC      (BamHI/BgIII/XHoI)
Product is pBjh1601AC-K112327

pBCa1601AC-K112328

Digest (BgIII methylated) K112227 AK, (BamHI methylated) K112102 KC      (BamHI/BgIII/XHoI)
Product is pBjh1601AC-K112328

pBCa1601AC-K112329

Digest  (BgIII methylated) K112230 AK, (BamHI methylated) K112113 CK      (BamHI/BgIII/XHoI)
Product is pBjh1601AC-K112329

pBCa1601AC-K112330

Digest K112614 AK (BgIII, XhoI)
Digest K112105 KC(BamHI, XhoI)
Product is pBjh1601AC-K112330

pBCa1601AC-K112331

Digest K112615 AK (BgIII, XhoI)
Digest K112108 KC(BamHI, XhoI)
Product is pBjh1601AC-K112331

pBCa1601AC-K112332

Digest  (BgIII methylated) K112615 AK, (BamHI methylated) K112216 KC      (BamHI/BgIII/XHoI)
Product is pBjh1601AC-K112332

pBCa1601AC-K112333

Digest (BgIII methylated) K112614 AK, (BamHI methylated) K112222 KC      (BamHI/BgIII/XHoI)
Product is pBjh1601AC-K112333

pBca1601AK-K112334

Digest (BgIII methylated) K112230 AC, (BamHI methylated) K112324 CK     (BamHI/BgIII/XHoI)
Product is pBjh1601AK-K112334

pBca1601AK-K112335

Digest (BgIII methylated) K112227 AC, (BamHI methylated) K112324 CK      (BamHI/BgIII/XHoI)
Product is pBjh1601AK-K112335

pBca1601AK-K112336

Digest (BgIII methylated) K112614 AC	  (BamHI/XhoI)
Digest (BamHI methylated) K112324 CK      (BgIII/XHoI)
Product is pBjh1601AK-K112336

pBca1601AK-K112337

Digest (BgIII methylated) K112615 AC, (BamHI methylated) K112324 CK      (BamHI/BgIII/XHoI)
Product is pBjh1601AK-K112337

pBca1601AK-K112339

Digest (BgIII methylated) K112327 AC     (BamHI/XhoI)
Digest (BamHI methylated) K112324 CK     (BgIII/XHoI)
Product is pBjh1601AK-K112339

pBca1601AK-K112340

Digest (BgIII methylated) K112328 AC, (BamHI methylated) K112324 CK      (BamHI/BgIII/XHoI)
Product is pBjh1601AK-K112340

pBca1601AK-K112341

Digest (BgIII methylated) K112329 AC, (BamHI methylated) K112324 CK      (BamHI/BgIII/XHoI)
Product is pBjh1601AK-K112341

pBca1601AK-K112342

Digest (BgIII methylated) K112330 AC, (BamHI methylated) K112324 CK      (BamHI/BgIII/XHoI)
Product is pBjh1601AK-K112342

pBca1601AK-K11234

Digest (BgIII methylated) K112332 AC, (BamHI methylated) K112324 CK     (BamHI/BgIII/XHoI)
Product is pBjh1601AK-K112344

pBca1601AK-K112348

Digest (BgIII methylated) K112328 AC, (BamHI methylated) K112325 CK     (BamHI/BgIII/XHoI)
Product is pBjh1601AK-K112348

pBca1601AK-K112349

Digest (BgIII methylated) K112329 AC, (BamHI methylated) K112325 CK     (BamHI/BgIII/XHoI)
Product is pBjh1601AK-K112349

pBca1601AK-K112350

Digest (BgIII methylated) K112330 AC	 (BamHI/XhoI)
Digest (BamHI methylated) K112325 CK     (BgIII/XHoI)
Product is pBjh1601AK-K112350

pBca1601AK-K112351

Digest (BgIII methylated) K112331 AC	 (BamHI/XhoI)
Digest (BamHI methylated) K112325 CK     (BgIII/XHoI)
Product is pBjh1601AK-K112351

pBca1601CK-K112354

Digest (BgIII methylated) K112900 CA, (BamHI methylated) K112334 AK     (BamHI/BgIII/XHoI)
Product is pBjh1601CK-K112354

pBca1601CK-K112355

Digest (BgIII methylated) K112900 CA, (BamHI methylated) K112335 AK     (BamHI/BgIII/XHoI)
Product is pBjh1601CK-K112354

pBca1601CK-K112356

Digest (BgIII methylated) K112900 CA, (BamHI methylated) K112336 AK     (BamHI/BgIII/XHoI)
Product is pBjh1601CK-K112354

pBca1601CK-K112357

Digest (BgIII methylated) K112900 CA, (BamHI methylated) K112337 AK     (BamHI/BgIII/XHoI)
Product is pBjh1601CK-K112354

pBca1601CK-K112360

Digest (BgIII methylated) K112900 CA, (BamHI methylated) K112340 AK     (BamHI/BgIII/XHoI)
Product is pBjh1601CK-K112360

pBca1601CK-K112361

Digest (BgIII methylated) K112900 CA, (BamHI methylated) K112341 AK     (BamHI/BgIII/XHoI)
Product is pBjh1601CK-K112361

pBca1601CK-K112362

Digest (BgIII methylated) K112900 CA, (BamHI methylated) K112342 AK     (BamHI/BgIII/XHoI)
Product is pBjh1601CK-K112362

pBca1601CK-K112363

Digest (BgIII methylated) K112900 CA, (BamHI methylated) K112343 AK     (BamHI/BgIII/XHoI)
Product is pBjh1601CK-K112363

pBca1601CK-K112368

Digest (BgIII methylated) K112900 CA, (BamHI methylated) K112348 AK     (BamHI/BgIII/XHoI)
Product is pBjh1601CK-K112368

pBca1601CK-K112369

Digest (BgIII methylated) K112900 CA, (BamHI methylated) K112349 AK     (BamHI/BgIII/XHoI)
Product is pBjh1601CK-K112369

pBca1601CK-K112370

Digest (BgIII methylated) K112900 CA, (BamHI methylated) K112350 AK     (BamHI/BgIII/XHoI)
Product is pBjh1601CK-K112370

pBca1601CK-K112371

Digest (BgIII methylated) K112900 CA, (BamHI methylated) K112351 AK     (BamHI/BgIII/XHoI)
Product is pBjh1601CK-K112371

pBca1601CK-K112372

Digest (BgIII methylated) K112121 CA, (BamHI methylated) K112709 AK     (BamHI/BgIII/XHoI)
Product is pBjh1601CK-K112372

pBca1601CK-K112373

Digest (BgIII methylated) BBa_K112232 CA, (BamHI methylated) K112709 AK     (BamHI/BgIII/XHoI)
Product is pBjh1601CK-K112373

@@ Line 1: / Line 1: @@
---[[User:Aronlau|Aronlau]] 01:15, 5 June 2008 (UTC)
+==pBca1256-K112300==
 <pre>
 PCR al0001/al0002 on pSB3C6-Bjh1501     (508bp, EcoRI/BamHI/DpnI)
@@ Line 10: / Line 10: @@
 CGttaGGATCCtcatacatcaatctctctgaccgttccgcc
 </pre>
+==pBca1256-K112301==
 <pre>
 PCR al0003/al0002 on pSB3C6-Bjh1501     (505bp, EcoRI/BamHI/DpnI)
@@ Line 21: / Line 21: @@
 CGttaGGATCCtcatacatcaatctctctgaccgttccgcc
 </pre>
+==pBca1256-K112302==
 <pre>
 PCR al0001/al0004 on pSB3C6-Bjh1501     (505bp, EcoRI/BamHI/DpnI)
@@ Line 32: / Line 32: @@
 CGttaGGATCCtacatcaatctctctgaccgttccgc
 </pre>
+==pBca1256-K112303==
 <pre>
 PCR al0003/al0004 on pSB3C6-Bjh1501     (502bp, EcoRI/BamHI/DpnI)
@@ Line 43: / Line 43: @@
 CGttaGGATCCtacatcaatctctctgaccgttccgc
 </pre>
+==pBca1256-K112304==
 <pre>
 PCR al0005/al0002 on pSB3C6-Bjh1501     (512bp, EcoRI/BamHI/DpnI)
@@ Line 54: / Line 54: @@
 CGttaGGATCCtcatacatcaatctctctgaccgttccgcc
 </pre>
+==pBca1256-K112305==
 <pre>
 PCR al0006/al0002 on pSB3C6-Bjh1501     (527bp, EcoRI/BamHI/DpnI)
@@ Line 65: / Line 65: @@
 CGttaGGATCCtcatacatcaatctctctgaccgttccgcc
 </pre>
+==pBca1256-K112306==
 <pre>
 PCR al0007/al0008 on pSB3C6-Bjh1502     (349bp, EcoRI/BamHI/DpnI)
@@ Line 76: / Line 76: @@
 CGttaGGATCCttattgatttctaccatcttctactccggc
 </pre>
+==pBca1256-K112307==
 <pre>
 PCR al0009/al0008 on pSB3C6-Bjh1502     (346bp, EcoRI/BamHI/DpnI)
@@ Line 87: / Line 87: @@
 CGttaGGATCCttattgatttctaccatcttctactccggc
 </pre>
+==pBca1256-K112308==
 <pre>
 PCR al0007/al0010 on pSB3C6-Bjh1502     (346bp, EcoRI/BamHI/DpnI)
@@ Line 98: / Line 98: @@
 CGttaGGATCCttgatttctaccatcttctactccggc
 </pre>
+==pBca1256-K112309==
 <pre>
 PCR al0009/al0010 on pSB3C6-Bjh1502     (343bp, EcoRI/BamHI/DpnI)
@@ Line 109: / Line 109: @@
 CGttaGGATCCttgatttctaccatcttctactccggc
 </pre>
+==pBca1256-K112310==
 <pre>
 PCR al0011/al0008 on pSB3C6-Bjh1502     (353bp, EcoRI/BamHI/DpnI)
@@ Line 120: / Line 120: @@
 CGttaGGATCCttattgatttctaccatcttctactccggc
 </pre>
+==pBca1256-K112311==
 <pre>
 PCR al0012/al0008 on pSB3C6-Bjh1502     (369bp, EcoRI/BamHI/DpnI)
@@ Line 127: / Line 127: @@
 -----------------------------------------------
 al0012	Forward Biobricking of lambda holin with rbs site
-CCATAGAATTCatgAGATCTattgggggtaagacCtgaagatgccag
+CCATAGAATTCatgAGATCTattgggggtaagacCtgaag
 al0008	Reverse Biobricking of lambda holin with rbs site
 CGttaGGATCCttattgatttctaccatcttctactccggc
 </pre>
+==pBca1256-K112312==
 <pre>
 PCR al0013/al0008 on pSB3C6-Bjh1503     (355bp, EcoRI/BamHI/DpnI)
@@ Line 142: / Line 142: @@
 CGttaGGATCCttattgatttctaccatcttctactccggc
 </pre>
+==pBca1256-K112313==
 <pre>
 PCR al0014/al0008 on pSB3C6-Bjh1503     (352bp, EcoRI/BamHI/DpnI)
@@ Line 153: / Line 153: @@
 CGttaGGATCCttattgatttctaccatcttctactccggc
 </pre>
+==pBca1256-K112314==
 <pre>
 PCR al0013/al0010 on pSB3C6-Bjh1503     (352bp, EcoRI/BamHI/DpnI)
@@ Line 164: / Line 164: @@
 CGttaGGATCCttgatttctaccatcttctactccggc
 </pre>
+==pBca1256-K112315==
 <pre>
 PCR al0014/al0010 on pSB3C6-Bjh1503     (349bp, EcoRI/BamHI/DpnI)
@@ Line 175: / Line 175: @@
 CGttaGGATCCttgatttctaccatcttctactccggc
 </pre>
+==pBca1256-K112316==
 <pre>
 PCR al0015/al0008 on pSB3C6-Bjh1503     (359bp, EcoRI/BamHI/DpnI)
@@ Line 186: / Line 186: @@
 CGttaGGATCCttattgatttctaccatcttctactccggc
 </pre>
+==pBca1256-K112317==
 <pre>
 PCR al0016/al0008 on pSB3C6-Bjh1503     (375bp, EcoRI/BamHI/DpnI)
@@ Line 193: / Line 193: @@
 -----------------------------------------------
 al0016	Forward Biobricking of lambda antiholin with rbs site
-CCATAGAATTCatgAGATCTccccttattgggggtaagacatgaagCtgccagaaaaacatgacc
+CCATAGAATTCatgAGATCTccccttattgggggtaagacatg
 al0008	Reverse Biobricking of lambda antiholin with rbs site
 CGttaGGATCCttattgatttctaccatcttctactccggc
 </pre>
+==pBca1256-K112318==
 <pre>
-PCR al0017/al0018      (467bp, EcoRI/BamHI/DpnI)
+PCR al0017/al0018 on MG1655     (467bp, EcoRI/BamHI/DpnI)
 Sub into pBca1256                      (EcoRI/BamHI, 2472+910, L)
 Product is pBca1256-K112318
@@ Line 209: / Line 209: @@
 </pre>
+==pBca1256-K112319==
 <pre>
-PCR al0019/al0020      (454bp, EcoRI/BamHI/DpnI)
+PCR al0019/al0024 on MG1655 (404bp, gp = A)
+PCR al0023/al0023 on MG1655 (86bp, gp=B)
+-----------------------------------------------
+PCR al0019/al0020 on A+B     (465bp, EcoRI/BamHI/DpnI)
 Sub into pBca1256                      (EcoRI/BamHI, 2472+910, L)
 Product is pBca1256-K112319
@@ Line 218: / Line 222: @@
 al0020	Reverse Biobricking of ftsQ promoter
 CGttaGGATCCcctcttcttcgctgtttcgc
+al0023 Forward Removing of EcoRI
+ggtagtacgaattGtggaactggcg
+al0024 Reverse Removing of EcoRI
+cgccagttccaCaattcgtactacc
 </pre>
+==pBca1256-K112320==
 <pre>
-PCR al0021/al0022      (804bp, EcoRI/BamHI/DpnI)
+PCR al0021/al0022 on MG1655     (804bp, EcoRI/BamHI/DpnI)
 Sub into pBca1256                      (EcoRI/BamHI, 2472+910, L)
 Product is pBca1256-K112320
@@ Line 229: / Line 238: @@
 al0022	Reverse Biobricking of ftsAZ promoter
 CGttaGGATCCgtaccaatctccagtcctactaccag
+</pre>
+==pBjh1601AC-K112321==
+<pre>
+PCR JH088F/JHO88R on Salmonella L2.     (435bp, EcoRI/BamHI/DpnI)
+Sub into pBjh1601AC                      (EcoRI/BamHI, 3195+910, L)
+Product is pBjh1601AC-K112321
+-----------------------------------------------
+JH088F   Forward biobricking of {H-NS!}
+cgataGAATTCatgAGATCTATGAGCGAAGCACTTAAAATTC
+JH088R   Reverse biobricking of {H-NS!}
+cgttaGGATCCTTATTGCTTGATCAGGAAATCG
+</pre>
+==pBjh1601CK-K112322==
+<pre>
+PCR JH089F/JH089R on Salmonella L2.    (379 bp, EcoRI/BamHI/DpnI)
+Sub into pBjh1601CK             (EcoRI/BamHI, 3134+910, L)
+Product is pBjh1601CK-K112322
+-----------------------------------------------
+JH089F   Forward biobricking of {Pdps}
+cgataGAATTCatgAGATCTaacgattcgacgcgaaccgc
+JH089R   Reverse biobricking of {Pdps}
+cgttaGGATCCaatctcatatcctcttgatg
+</pre>
+==pBCa1601AC-K112323==
+<pre>
+PCR ca998/JH090R on pBjh1601AC-K112321-2    (445 bp, EcoRI/BamHI)
+Sub into pBjh1601AC                      (EcoRI/BamHI, 3195+910, L)
+Product is pBjh1601AC-K112323
+-----------------------------------------------
+ca998   Forward biobricking of {H-NS!} Salmonella
+gtatcacgaggcagaatttcag
+JH090R   Reverse biobricking of {H-NS!} Salmonella
+cgttaGGATCCTTATTcCTTGATCAGG
+</pre>
+==pBCa1601CK-K112324==
+<pre>
+Digest (BgIII methylated) K112902 CA, (BamHI methylated) K112709 AK      (BamHI/BgIII/XHoI)
+Product is pBjh1601CK-K112324
+</pre>
+==pBCa1601CK-K112325==
+<pre>
+Digest (BgIII methylated) K112704 CA, (BamHI methylated) K112709 AK      (BamHI/BgIII/XHoI)
+Product is pBjh1601CK-K112325
+</pre>
+==pBCa1601AC-K112327==
+<pre>
+Digest (BgIII methylated) K112230 AK, (BamHI methylated) K112203 KC      (BamHI/BgIII/XHoI)
+Product is pBjh1601AC-K112327
+</pre>
+==pBCa1601AC-K112328==
+<pre>
+Digest (BgIII methylated) K112227 AK, (BamHI methylated) K112102 KC      (BamHI/BgIII/XHoI)
+Product is pBjh1601AC-K112328
+</pre>
+==pBCa1601AC-K112329==
+<pre>
+Digest  (BgIII methylated) K112230 AK, (BamHI methylated) K112113 CK      (BamHI/BgIII/XHoI)
+Product is pBjh1601AC-K112329
+</pre>
+==pBCa1601AC-K112330==
+<pre>
+Digest K112614 AK (BgIII, XhoI)
+Digest K112105 KC(BamHI, XhoI)
+Product is pBjh1601AC-K112330
+</pre>
+==pBCa1601AC-K112331==
+<pre>
+Digest K112615 AK (BgIII, XhoI)
+Digest K112108 KC(BamHI, XhoI)
+Product is pBjh1601AC-K112331
+</pre>
+==pBCa1601AC-K112332==
+<pre>
+Digest  (BgIII methylated) K112615 AK, (BamHI methylated) K112216 KC      (BamHI/BgIII/XHoI)
+Product is pBjh1601AC-K112332
+</pre>
+==pBCa1601AC-K112333==
+<pre>
+Digest (BgIII methylated) K112614 AK, (BamHI methylated) K112222 KC      (BamHI/BgIII/XHoI)
+Product is pBjh1601AC-K112333
+</pre>
+==pBca1601AK-K112334==
+<pre>
+Digest (BgIII methylated) K112230 AC, (BamHI methylated) K112324 CK     (BamHI/BgIII/XHoI)
+Product is pBjh1601AK-K112334
+</pre>
+==pBca1601AK-K112335==
+<pre>
+Digest (BgIII methylated) K112227 AC, (BamHI methylated) K112324 CK      (BamHI/BgIII/XHoI)
+Product is pBjh1601AK-K112335
+</pre>
+==pBca1601AK-K112336==
+<pre>
+Digest (BgIII methylated) K112614 AC	  (BamHI/XhoI)
+Digest (BamHI methylated) K112324 CK      (BgIII/XHoI)
+Product is pBjh1601AK-K112336
+</pre>
+==pBca1601AK-K112337==
+<pre>
+Digest (BgIII methylated) K112615 AC, (BamHI methylated) K112324 CK      (BamHI/BgIII/XHoI)
+Product is pBjh1601AK-K112337
+</pre>
+==pBca1601AK-K112339==
+<pre>
+Digest (BgIII methylated) K112327 AC     (BamHI/XhoI)
+Digest (BamHI methylated) K112324 CK     (BgIII/XHoI)
+Product is pBjh1601AK-K112339
+</pre>
+==pBca1601AK-K112340==
+<pre>
+Digest (BgIII methylated) K112328 AC, (BamHI methylated) K112324 CK      (BamHI/BgIII/XHoI)
+Product is pBjh1601AK-K112340
+</pre>
+==pBca1601AK-K112341==
+<pre>
+Digest (BgIII methylated) K112329 AC, (BamHI methylated) K112324 CK      (BamHI/BgIII/XHoI)
+Product is pBjh1601AK-K112341
+</pre>
+==pBca1601AK-K112342==
+<pre>
+Digest (BgIII methylated) K112330 AC, (BamHI methylated) K112324 CK      (BamHI/BgIII/XHoI)
+Product is pBjh1601AK-K112342
+</pre>
+==pBca1601AK-K11234==
+<pre>
+Digest (BgIII methylated) K112332 AC, (BamHI methylated) K112324 CK     (BamHI/BgIII/XHoI)
+Product is pBjh1601AK-K112344
+</pre>
+==pBca1601AK-K112348==
+<pre>
+Digest (BgIII methylated) K112328 AC, (BamHI methylated) K112325 CK     (BamHI/BgIII/XHoI)
+Product is pBjh1601AK-K112348
+</pre>
+==pBca1601AK-K112349==
+<pre>
+Digest (BgIII methylated) K112329 AC, (BamHI methylated) K112325 CK     (BamHI/BgIII/XHoI)
+Product is pBjh1601AK-K112349
+</pre>
+==pBca1601AK-K112350==
+<pre>
+Digest (BgIII methylated) K112330 AC	 (BamHI/XhoI)
+Digest (BamHI methylated) K112325 CK     (BgIII/XHoI)
+Product is pBjh1601AK-K112350
+</pre>
+==pBca1601AK-K112351==
+<pre>
+Digest (BgIII methylated) K112331 AC	 (BamHI/XhoI)
+Digest (BamHI methylated) K112325 CK     (BgIII/XHoI)
+Product is pBjh1601AK-K112351
+</pre>
+==pBca1601CK-K112354==
+<pre>
+Digest (BgIII methylated) K112900 CA, (BamHI methylated) K112334 AK     (BamHI/BgIII/XHoI)
+Product is pBjh1601CK-K112354
+</pre>
+==pBca1601CK-K112355==
+<pre>
+Digest (BgIII methylated) K112900 CA, (BamHI methylated) K112335 AK     (BamHI/BgIII/XHoI)
+Product is pBjh1601CK-K112354
+</pre>
+==pBca1601CK-K112356==
+<pre>
+Digest (BgIII methylated) K112900 CA, (BamHI methylated) K112336 AK     (BamHI/BgIII/XHoI)
+Product is pBjh1601CK-K112354
+</pre>
+==pBca1601CK-K112357==
+<pre>
+Digest (BgIII methylated) K112900 CA, (BamHI methylated) K112337 AK     (BamHI/BgIII/XHoI)
+Product is pBjh1601CK-K112354
+</pre>
+==pBca1601CK-K112360==
+<pre>
+Digest (BgIII methylated) K112900 CA, (BamHI methylated) K112340 AK     (BamHI/BgIII/XHoI)
+Product is pBjh1601CK-K112360
+</pre>
+==pBca1601CK-K112361==
+<pre>
+Digest (BgIII methylated) K112900 CA, (BamHI methylated) K112341 AK     (BamHI/BgIII/XHoI)
+Product is pBjh1601CK-K112361
+</pre>
+==pBca1601CK-K112362==
+<pre>
+Digest (BgIII methylated) K112900 CA, (BamHI methylated) K112342 AK     (BamHI/BgIII/XHoI)
+Product is pBjh1601CK-K112362
+</pre>
+==pBca1601CK-K112363==
+<pre>
+Digest (BgIII methylated) K112900 CA, (BamHI methylated) K112343 AK     (BamHI/BgIII/XHoI)
+Product is pBjh1601CK-K112363
+</pre>
+==pBca1601CK-K112368==
+<pre>
+Digest (BgIII methylated) K112900 CA, (BamHI methylated) K112348 AK     (BamHI/BgIII/XHoI)
+Product is pBjh1601CK-K112368
+</pre>
+==pBca1601CK-K112369==
+<pre>
+Digest (BgIII methylated) K112900 CA, (BamHI methylated) K112349 AK     (BamHI/BgIII/XHoI)
+Product is pBjh1601CK-K112369
+</pre>
+==pBca1601CK-K112370==
+<pre>
+Digest (BgIII methylated) K112900 CA, (BamHI methylated) K112350 AK     (BamHI/BgIII/XHoI)
+Product is pBjh1601CK-K112370
+</pre>
+==pBca1601CK-K112371==
+<pre>
+Digest (BgIII methylated) K112900 CA, (BamHI methylated) K112351 AK     (BamHI/BgIII/XHoI)
+Product is pBjh1601CK-K112371
+</pre>
+==pBca1601CK-K112372==
+<pre>
+Digest (BgIII methylated) K112121 CA, (BamHI methylated) K112709 AK     (BamHI/BgIII/XHoI)
+Product is pBjh1601CK-K112372
+</pre>
+==pBca1601CK-K112373==
+<pre>
+Digest (BgIII methylated) BBa_K112232 CA, (BamHI methylated) K112709 AK     (BamHI/BgIII/XHoI)
+Product is pBjh1601CK-K112373
 </pre>

Template:Team:UC Berkeley/Notebook/AL construction

From 2008.igem.org

Latest revision as of 02:10, 29 October 2008

Contents

pBca1256-K112300

pBca1256-K112301

pBca1256-K112302

pBca1256-K112303

pBca1256-K112304

pBca1256-K112305

pBca1256-K112306

pBca1256-K112307

pBca1256-K112308

pBca1256-K112309

pBca1256-K112310

pBca1256-K112311

pBca1256-K112312

pBca1256-K112313

pBca1256-K112314

pBca1256-K112315

pBca1256-K112316

pBca1256-K112317

pBca1256-K112318

pBca1256-K112319

pBca1256-K112320

pBjh1601AC-K112321

pBjh1601CK-K112322

pBCa1601AC-K112323

pBCa1601CK-K112324

pBCa1601CK-K112325

pBCa1601AC-K112327

pBCa1601AC-K112328

pBCa1601AC-K112329

pBCa1601AC-K112330

pBCa1601AC-K112331

pBCa1601AC-K112332

pBCa1601AC-K112333

pBca1601AK-K112334

pBca1601AK-K112335

pBca1601AK-K112336

pBca1601AK-K112337

pBca1601AK-K112339

pBca1601AK-K112340

pBca1601AK-K112341

pBca1601AK-K112342

pBca1601AK-K11234

pBca1601AK-K112348

pBca1601AK-K112349

pBca1601AK-K112350

pBca1601AK-K112351

pBca1601CK-K112354

pBca1601CK-K112355

pBca1601CK-K112356

pBca1601CK-K112357

pBca1601CK-K112360

pBca1601CK-K112361

pBca1601CK-K112362

pBca1601CK-K112363

pBca1601CK-K112368

pBca1601CK-K112369

pBca1601CK-K112370

pBca1601CK-K112371

pBca1601CK-K112372

pBca1601CK-K112373