Team:Caltech/Project/Vitamins/Parts

From 2008.igem.org

(Difference between revisions)
(New page: {{Caltech_iGEM_08| Content= <div style="font-size:18pt;"> <font face="verdana" style="color:#CC3300">Folate Production Parts</font></div> <br> ==Basic Parts== {| border="1" |+ Basic Parts...)
 
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==Basic Parts==
==Basic Parts==
-
{| border="1"
+
{{{{!}}}border="1"
-
|+ Basic Parts (Extracted from ''L.lactis subspe. IL1403'' genome)
+
{{{!}}}+ Basic Parts (Extracted from ''L.lactis subspe. IL1403'' genome)
! Part Name !! Registry # !! Description !! Cloned? !! Sequence confirmed?   
! Part Name !! Registry # !! Description !! Cloned? !! Sequence confirmed?   
-
|-
+
{{{!}}}-
! Entire folate synthesis operon
! Entire folate synthesis operon
-
| [http://partsregistry.org/Part:BBa_K137002 BBa_K137002]|| Includes folB+folKE+folP+ylgG+folC || NO || NO
+
{{{!}}} [http://partsregistry.org/Part:BBa_K137002 BBa_K137002]{{{!}}}{{{!}}} Includes folB+folKE+folP+ylgG+folC {{{!}}}{{{!}}} NO {{{!}}}{{{!}}} NO
-
|-
+
{{{!}}}-
! folB
! folB
-
| [http://partsregistry.org/Part:BBa_K137009 BBa_K137009]|| dihydroneopterin aldolase || YES|| YES
+
{{{!}}} [http://partsregistry.org/Part:BBa_K137009 BBa_K137009]{{{!}}}{{{!}}} dihydroneopterin aldolase {{{!}}}{{{!}}} YES{{{!}}}{{{!}}} YES
-
|-
+
{{{!}}}-
! folKE
! folKE
-
| [http://partsregistry.org/Part:BBa_K137011 BBa_K137011]|| fusion gene: GTP cyclohydrolase & 2-amino-4-hydroxy-6- hydroxymethyldihydropteridine pyrophosphokinase  || YES|| YES
+
{{{!}}} [http://partsregistry.org/Part:BBa_K137011 BBa_K137011]{{{!}}}{{{!}}} fusion gene: GTP cyclohydrolase & 2-amino-4-hydroxy-6- hydroxymethyldihydropteridine pyrophosphokinase  {{{!}}}{{{!}}} YES{{{!}}}{{{!}}} YES
-
|-
+
{{{!}}}-
! folP
! folP
-
| [http://partsregistry.org/Part:BBa_K137012 BBa_K137012]|| Dihydropteroate synthase  || NO|| NO
+
{{{!}}} [http://partsregistry.org/Part:BBa_K137012 BBa_K137012]{{{!}}}{{{!}}} Dihydropteroate synthase  {{{!}}}{{{!}}} NO{{{!}}}{{{!}}} NO
-
|-
+
{{{!}}}-
! folC
! folC
-
| [http://partsregistry.org/Part:BBa_K137013 BBa_K137013]|| folate synthetase/polyglutamyl folate synthetase  || NO|| NO
+
{{{!}}} [http://partsregistry.org/Part:BBa_K137013 BBa_K137013]{{{!}}}{{{!}}} folate synthetase/polyglutamyl folate synthetase  {{{!}}}{{{!}}} NO{{{!}}}{{{!}}} NO
-
|-
+
{{{!}}}-
! pabA
! pabA
-
| [http://partsregistry.org/Part:BBa_K137005 BBa_K137005]|| para-aminobenzoate synthetase component II || YES|| YES
+
{{{!}}} [http://partsregistry.org/Part:BBa_K137005 BBa_K137005]{{{!}}}{{{!}}} para-aminobenzoate synthetase component II {{{!}}}{{{!}}} YES{{{!}}}{{{!}}} YES
-
|-
+
{{{!}}}-
! pabB
! pabB
-
| [http://partsregistry.org/Part:BBa_K137006 BBa_K137006]|| para-aminobenzoate synthetase component I || YES|| YES
+
{{{!}}} [http://partsregistry.org/Part:BBa_K137006 BBa_K137006]{{{!}}}{{{!}}} para-aminobenzoate synthetase component I {{{!}}}{{{!}}} YES{{{!}}}{{{!}}} YES
-
|-
+
{{{!}}}-
-
|}
+
{{{!}}}}
==Construction Intermediates==
==Construction Intermediates==
-
{| border="1"
+
{{{{!}}} border="1"
-
|+ Construction Intermediates: Adding b0034 (strong RBS) before each individual gene
+
{{{!}}}+ Construction Intermediates: Adding b0034 (strong RBS) before each individual gene
! Part Name !! Registry # !! Description !! Cloned? !! Sequence confirmed?   
! Part Name !! Registry # !! Description !! Cloned? !! Sequence confirmed?   
-
|-
+
{{{!}}}-
! folB + b0034
! folB + b0034
-
| [http://partsregistry.org/Part:BBa_S03957 BBa_S03957]|| RBS + dihydroneopterin aldolase || YES|| YES
+
{{{!}}} [http://partsregistry.org/Part:BBa_S03957 BBa_S03957]{{{!}}}{{{!}}} RBS + dihydroneopterin aldolase {{{!}}}{{{!}}} YES{{{!}}}{{{!}}} YES
-
|-
+
{{{!}}}-
! folKE + b0034
! folKE + b0034
-
| [http://partsregistry.org/Part:BBa_S03958 BBa_S03958]|| RBS + fusion gene: GTP cyclohydrolase & 2-amino-4-hydroxy-6- hydroxymethyldihydropteridine pyrophosphokinase  || YES|| YES
+
{{{!}}} [http://partsregistry.org/Part:BBa_S03958 BBa_S03958]{{{!}}}{{{!}}} RBS + fusion gene: GTP cyclohydrolase & 2-amino-4-hydroxy-6- hydroxymethyldihydropteridine pyrophosphokinase  {{{!}}}{{{!}}} YES{{{!}}}{{{!}}} YES
-
|-
+
{{{!}}}-
! folP + b0034
! folP + b0034
-
| [http://partsregistry.org/Part:BBa_S03959 BBa_S03959]|| RBS + Dihydropteroate synthase  || NO|| NO
+
{{{!}}} [http://partsregistry.org/Part:BBa_S03959 BBa_S03959]{{{!}}}{{{!}}} RBS + Dihydropteroate synthase  {{{!}}}{{{!}}} NO{{{!}}}{{{!}}} NO
-
|-
+
{{{!}}}-
! folC + b0034
! folC + b0034
-
| [http://partsregistry.org/Part:BBa_S03960 BBa_S03960]|| RBS + folate synthetase/polyglutamyl folate synthetase  || NO|| NO
+
{{{!}}} [http://partsregistry.org/Part:BBa_S03960 BBa_S03960]{{{!}}}{{{!}}} RBS + folate synthetase/polyglutamyl folate synthetase  {{{!}}}{{{!}}} NO{{{!}}}{{{!}}} NO
-
|-
+
{{{!}}}-
! pabA + b0034
! pabA + b0034
-
| [http://partsregistry.org/Part:BBa_S03976 BBa_S03976]|| RBS + para-aminobenzoate synthetase component II || YES|| YES
+
{{{!}}} [http://partsregistry.org/Part:BBa_S03976 BBa_S03976]{{{!}}}{{{!}}} RBS + para-aminobenzoate synthetase component II {{{!}}}{{{!}}} YES{{{!}}}{{{!}}} YES
-
|-
+
{{{!}}}-
! pabB + b0034
! pabB + b0034
-
| [http://partsregistry.org/Part:BBa_S03977 BBa_S03977]|| RBS + para-aminobenzoate synthetase component I || YES|| YES
+
{{{!}}} [http://partsregistry.org/Part:BBa_S03977 BBa_S03977]{{{!}}}{{{!}}} RBS + para-aminobenzoate synthetase component I {{{!}}}{{{!}}} YES{{{!}}}{{{!}}} YES
-
|-
+
{{{!}}}-
! b0034 + folB + b0034 + folKE  
! b0034 + folB + b0034 + folKE  
-
| [http://partsregistry.org/Part:BBa_S03961 BBa_S03961]|| Combining folB (with RBS) + folKE (with RBS) || YES|| YES
+
{{{!}}} [http://partsregistry.org/Part:BBa_S03961 BBa_S03961]{{{!}}}{{{!}}} Combining folB (with RBS) + folKE (with RBS) {{{!}}}{{{!}}} YES{{{!}}}{{{!}}} YES
-
|-
+
{{{!}}}-
-
|}
+
{{{!}}}}
-
{| border="1"
+
{{{{!}}} border="1"
-
|+ Construction Intermediates: Adding j23100 (constitutive promoter) to each gene (with RBS already)
+
{{{!}}}+ Construction Intermediates: Adding j23100 (constitutive promoter) to each gene (with RBS already)
! Part Name !! Registry # !! Description !! Cloned? !! Sequence confirmed?   
! Part Name !! Registry # !! Description !! Cloned? !! Sequence confirmed?   
-
|-
+
{{{!}}}-
! folB + j23100
! folB + j23100
-
| [http://partsregistry.org/Part:BBa_S04032 BBa_S04032]|| Promoter(j23100) + RBS(b0034) + dihydroneopterin aldolase || YES|| YES
+
{{{!}}} [http://partsregistry.org/Part:BBa_S04032 BBa_S04032]{{{!}}}{{{!}}} Promoter(j23100) + RBS(b0034) + dihydroneopterin aldolase {{{!}}}{{{!}}} YES{{{!}}}{{{!}}} YES
-
|-
+
{{{!}}}-
! folKE + j23100
! folKE + j23100
-
| [http://partsregistry.org/Part:BBa_S04033 BBa_S04033]|| Promoter + RBS + fusion gene: GTP cyclohydrolase & 2-amino-4-hydroxy-6- hydroxymethyldihydropteridine pyrophosphokinase  || YES|| YES
+
{{{!}}} [http://partsregistry.org/Part:BBa_S04033 BBa_S04033]{{{!}}}{{{!}}} Promoter + RBS + fusion gene: GTP cyclohydrolase & 2-amino-4-hydroxy-6- hydroxymethyldihydropteridine pyrophosphokinase  {{{!}}}{{{!}}} YES{{{!}}}{{{!}}} YES
-
|-
+
{{{!}}}-
! pabA + j23100
! pabA + j23100
-
| [http://partsregistry.org/Part:BBa_S04034 BBa_S04034]|| Promoter + RBS + para-aminobenzoate synthetase component II || YES|| YES
+
{{{!}}} [http://partsregistry.org/Part:BBa_S04034 BBa_S04034]{{{!}}}{{{!}}} Promoter + RBS + para-aminobenzoate synthetase component II {{{!}}}{{{!}}} YES{{{!}}}{{{!}}} YES
-
|-
+
{{{!}}}-
! folBKE + j23100
! folBKE + j23100
-
| [http://partsregistry.org/Part:BBa_S04035 BBa_S04035]|| Promoter + folB (with RBS) + folKE (with RBS) || YES || YES
+
{{{!}}} [http://partsregistry.org/Part:BBa_S04035 BBa_S04035]{{{!}}}{{{!}}} Promoter + folB (with RBS) + folKE (with RBS) {{{!}}}{{{!}}} YES {{{!}}}{{{!}}} YES
-
|-
+
{{{!}}}-
! pabB + j23100
! pabB + j23100
-
| [http://partsregistry.org/Part:BBa_S04039 BBa_S04039]|| Promoter + pabB  || YES || YES  
+
{{{!}}} [http://partsregistry.org/Part:BBa_S04039 BBa_S04039]{{{!}}}{{{!}}} Promoter + pabB  {{{!}}}{{{!}}} YES {{{!}}}{{{!}}} YES  
-
|-
+
{{{!}}}-
! pabB + b0015
! pabB + b0015
-
| [http://partsregistry.org/Part:BBa_S04038 BBa_S04038]|| pabB + b0015 terminator || MAYBE || WAITING
+
{{{!}}} [http://partsregistry.org/Part:BBa_S04038 BBa_S04038]{{{!}}}{{{!}}} pabB + b0015 terminator {{{!}}}{{{!}}} MAYBE {{{!}}}{{{!}}} WAITING
-
|-
+
{{{!}}}-
-
|}
+
{{{!}}}}
===Composite Parts===
===Composite Parts===
-
{| border="1"
+
{{{{!}}} border="1"
-
|+ Composite Parts: Adding b0015 (double terminator) to complete constructs with promoter + RBS already
+
{{{!}}}+ Composite Parts: Adding b0015 (double terminator) to complete constructs with promoter + RBS already
! Part Name !! Registry # !! Description !! Cloned? !! Sequence confirmed?   
! Part Name !! Registry # !! Description !! Cloned? !! Sequence confirmed?   
-
|-
+
{{{!}}}-
! folB + b0015
! folB + b0015
-
| [http://partsregistry.org/Part:BBa_K137053 BBa_K137053]|| Promoter(j23100) + RBS(b0034) + folB (dihydroneopterin aldolase) + double terminator (b0015) || YES || YES
+
{{{!}}} [http://partsregistry.org/Part:BBa_K137053 BBa_K137053]{{{!}}}{{{!}}} Promoter(j23100) + RBS(b0034) + folB (dihydroneopterin aldolase) + double terminator (b0015) {{{!}}}{{{!}}} YES {{{!}}}{{{!}}} YES
-
|-
+
{{{!}}}-
! folKE + b0015
! folKE + b0015
-
| [http://partsregistry.org/Part:BBa_K137054 BBa_K137054]|| Promoter + RBS + folKE (fusion gene: GTP cyclohydrolase & 2-amino-4-hydroxy-6- hydroxymethyldihydropteridine pyrophosphokinase) + double terminator (b0015)  || YES || YES
+
{{{!}}} [http://partsregistry.org/Part:BBa_K137054 BBa_K137054]{{{!}}}{{{!}}} Promoter + RBS + folKE (fusion gene: GTP cyclohydrolase & 2-amino-4-hydroxy-6- hydroxymethyldihydropteridine pyrophosphokinase) + double terminator (b0015)  {{{!}}}{{{!}}} YES {{{!}}}{{{!}}} YES
-
|-
+
{{{!}}}-
! pabA + b0015
! pabA + b0015
-
| [http://partsregistry.org/Part:BBa_K137055 BBa_K137055]|| Promoter + RBS + pabA (para-aminobenzoate synthetase component II) || YES  || YES
+
{{{!}}} [http://partsregistry.org/Part:BBa_K137055 BBa_K137055]{{{!}}}{{{!}}} Promoter + RBS + pabA (para-aminobenzoate synthetase component II) {{{!}}}{{{!}}} YES  {{{!}}}{{{!}}} YES
-
|-
+
{{{!}}}-
! pabB + b0015
! pabB + b0015
-
| [http://partsregistry.org/Part:BBa_K137109 BBa_K137109]|| Promoter + RBS + pabB (para-aminobenzoate synthetase component I) || YES  || NO
+
{{{!}}} [http://partsregistry.org/Part:BBa_K137109 BBa_K137109]{{{!}}}{{{!}}} Promoter + RBS + pabB (para-aminobenzoate synthetase component I) {{{!}}}{{{!}}} YES  {{{!}}}{{{!}}} NO
-
|-
+
{{{!}}}-
! folBKE + b0015
! folBKE + b0015
-
| [http://partsregistry.org/Part:BBa_K137056 BBa_K137056]|| Promoter + RBS + folB + folKE + b0015 || YES || NO
+
{{{!}}} [http://partsregistry.org/Part:BBa_K137056 BBa_K137056]{{{!}}}{{{!}}} Promoter + RBS + folB + folKE + b0015 {{{!}}}{{{!}}} YES {{{!}}}{{{!}}} NO
-
|-
+
{{{!}}}-
-
|}
+
{{{!}}}}
}}
}}

Latest revision as of 00:13, 11 September 2008


iGEM 2008



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Folate Production Parts


Basic Parts

{{{{!}}}border="1" {{{!}}}+ Basic Parts (Extracted from L.lactis subspe. IL1403 genome)

Part Name Registry # Description Cloned? Sequence confirmed?

{{{!}}}-

Entire folate synthesis operon

{{{!}}} [http://partsregistry.org/Part:BBa_K137002 BBa_K137002]{{{!}}}{{{!}}} Includes folB+folKE+folP+ylgG+folC {{{!}}}{{{!}}} NO {{{!}}}{{{!}}} NO {{{!}}}-

folB

{{{!}}} [http://partsregistry.org/Part:BBa_K137009 BBa_K137009]{{{!}}}{{{!}}} dihydroneopterin aldolase {{{!}}}{{{!}}} YES{{{!}}}{{{!}}} YES {{{!}}}-

folKE

{{{!}}} [http://partsregistry.org/Part:BBa_K137011 BBa_K137011]{{{!}}}{{{!}}} fusion gene: GTP cyclohydrolase & 2-amino-4-hydroxy-6- hydroxymethyldihydropteridine pyrophosphokinase {{{!}}}{{{!}}} YES{{{!}}}{{{!}}} YES {{{!}}}-

folP

{{{!}}} [http://partsregistry.org/Part:BBa_K137012 BBa_K137012]{{{!}}}{{{!}}} Dihydropteroate synthase {{{!}}}{{{!}}} NO{{{!}}}{{{!}}} NO {{{!}}}-

folC

{{{!}}} [http://partsregistry.org/Part:BBa_K137013 BBa_K137013]{{{!}}}{{{!}}} folate synthetase/polyglutamyl folate synthetase {{{!}}}{{{!}}} NO{{{!}}}{{{!}}} NO {{{!}}}-

pabA

{{{!}}} [http://partsregistry.org/Part:BBa_K137005 BBa_K137005]{{{!}}}{{{!}}} para-aminobenzoate synthetase component II {{{!}}}{{{!}}} YES{{{!}}}{{{!}}} YES {{{!}}}-

pabB

{{{!}}} [http://partsregistry.org/Part:BBa_K137006 BBa_K137006]{{{!}}}{{{!}}} para-aminobenzoate synthetase component I {{{!}}}{{{!}}} YES{{{!}}}{{{!}}} YES {{{!}}}- {{{!}}}}

Construction Intermediates

{{{{!}}} border="1" {{{!}}}+ Construction Intermediates: Adding b0034 (strong RBS) before each individual gene

Part Name Registry # Description Cloned? Sequence confirmed?

{{{!}}}-

folB + b0034

{{{!}}} [http://partsregistry.org/Part:BBa_S03957 BBa_S03957]{{{!}}}{{{!}}} RBS + dihydroneopterin aldolase {{{!}}}{{{!}}} YES{{{!}}}{{{!}}} YES {{{!}}}-

folKE + b0034

{{{!}}} [http://partsregistry.org/Part:BBa_S03958 BBa_S03958]{{{!}}}{{{!}}} RBS + fusion gene: GTP cyclohydrolase & 2-amino-4-hydroxy-6- hydroxymethyldihydropteridine pyrophosphokinase {{{!}}}{{{!}}} YES{{{!}}}{{{!}}} YES {{{!}}}-

folP + b0034

{{{!}}} [http://partsregistry.org/Part:BBa_S03959 BBa_S03959]{{{!}}}{{{!}}} RBS + Dihydropteroate synthase {{{!}}}{{{!}}} NO{{{!}}}{{{!}}} NO {{{!}}}-

folC + b0034

{{{!}}} [http://partsregistry.org/Part:BBa_S03960 BBa_S03960]{{{!}}}{{{!}}} RBS + folate synthetase/polyglutamyl folate synthetase {{{!}}}{{{!}}} NO{{{!}}}{{{!}}} NO {{{!}}}-

pabA + b0034

{{{!}}} [http://partsregistry.org/Part:BBa_S03976 BBa_S03976]{{{!}}}{{{!}}} RBS + para-aminobenzoate synthetase component II {{{!}}}{{{!}}} YES{{{!}}}{{{!}}} YES {{{!}}}-

pabB + b0034

{{{!}}} [http://partsregistry.org/Part:BBa_S03977 BBa_S03977]{{{!}}}{{{!}}} RBS + para-aminobenzoate synthetase component I {{{!}}}{{{!}}} YES{{{!}}}{{{!}}} YES {{{!}}}-

b0034 + folB + b0034 + folKE

{{{!}}} [http://partsregistry.org/Part:BBa_S03961 BBa_S03961]{{{!}}}{{{!}}} Combining folB (with RBS) + folKE (with RBS) {{{!}}}{{{!}}} YES{{{!}}}{{{!}}} YES {{{!}}}- {{{!}}}}

{{{{!}}} border="1" {{{!}}}+ Construction Intermediates: Adding j23100 (constitutive promoter) to each gene (with RBS already)

Part Name Registry # Description Cloned? Sequence confirmed?

{{{!}}}-

folB + j23100

{{{!}}} [http://partsregistry.org/Part:BBa_S04032 BBa_S04032]{{{!}}}{{{!}}} Promoter(j23100) + RBS(b0034) + dihydroneopterin aldolase {{{!}}}{{{!}}} YES{{{!}}}{{{!}}} YES {{{!}}}-

folKE + j23100

{{{!}}} [http://partsregistry.org/Part:BBa_S04033 BBa_S04033]{{{!}}}{{{!}}} Promoter + RBS + fusion gene: GTP cyclohydrolase & 2-amino-4-hydroxy-6- hydroxymethyldihydropteridine pyrophosphokinase {{{!}}}{{{!}}} YES{{{!}}}{{{!}}} YES {{{!}}}-

pabA + j23100

{{{!}}} [http://partsregistry.org/Part:BBa_S04034 BBa_S04034]{{{!}}}{{{!}}} Promoter + RBS + para-aminobenzoate synthetase component II {{{!}}}{{{!}}} YES{{{!}}}{{{!}}} YES {{{!}}}-

folBKE + j23100

{{{!}}} [http://partsregistry.org/Part:BBa_S04035 BBa_S04035]{{{!}}}{{{!}}} Promoter + folB (with RBS) + folKE (with RBS) {{{!}}}{{{!}}} YES {{{!}}}{{{!}}} YES {{{!}}}-

pabB + j23100

{{{!}}} [http://partsregistry.org/Part:BBa_S04039 BBa_S04039]{{{!}}}{{{!}}} Promoter + pabB {{{!}}}{{{!}}} YES {{{!}}}{{{!}}} YES {{{!}}}-

pabB + b0015

{{{!}}} [http://partsregistry.org/Part:BBa_S04038 BBa_S04038]{{{!}}}{{{!}}} pabB + b0015 terminator {{{!}}}{{{!}}} MAYBE {{{!}}}{{{!}}} WAITING {{{!}}}- {{{!}}}}

Composite Parts

{{{{!}}} border="1" {{{!}}}+ Composite Parts: Adding b0015 (double terminator) to complete constructs with promoter + RBS already

Part Name Registry # Description Cloned? Sequence confirmed?

{{{!}}}-

folB + b0015

{{{!}}} [http://partsregistry.org/Part:BBa_K137053 BBa_K137053]{{{!}}}{{{!}}} Promoter(j23100) + RBS(b0034) + folB (dihydroneopterin aldolase) + double terminator (b0015) {{{!}}}{{{!}}} YES {{{!}}}{{{!}}} YES {{{!}}}-

folKE + b0015

{{{!}}} [http://partsregistry.org/Part:BBa_K137054 BBa_K137054]{{{!}}}{{{!}}} Promoter + RBS + folKE (fusion gene: GTP cyclohydrolase & 2-amino-4-hydroxy-6- hydroxymethyldihydropteridine pyrophosphokinase) + double terminator (b0015) {{{!}}}{{{!}}} YES {{{!}}}{{{!}}} YES {{{!}}}-

pabA + b0015

{{{!}}} [http://partsregistry.org/Part:BBa_K137055 BBa_K137055]{{{!}}}{{{!}}} Promoter + RBS + pabA (para-aminobenzoate synthetase component II) {{{!}}}{{{!}}} YES {{{!}}}{{{!}}} YES {{{!}}}-

pabB + b0015

{{{!}}} [http://partsregistry.org/Part:BBa_K137109 BBa_K137109]{{{!}}}{{{!}}} Promoter + RBS + pabB (para-aminobenzoate synthetase component I) {{{!}}}{{{!}}} YES {{{!}}}{{{!}}} NO {{{!}}}-

folBKE + b0015

{{{!}}} [http://partsregistry.org/Part:BBa_K137056 BBa_K137056]{{{!}}}{{{!}}} Promoter + RBS + folB + folKE + b0015 {{{!}}}{{{!}}} YES {{{!}}}{{{!}}} NO {{{!}}}- {{{!}}}}

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