Latest revision as of 21:27, 29 October 2008

A novel system for generation and screening for new interactors targeted against any bait

maximization of interaction between given bait and randomly generated protein by mutating sequence of the latter
interactor presentation on bacterial surface
selection of best bait interactor

Institutions whom we would like to thank for financial and technical support:

University of Warsaw	Faculty of Biology, University of Warsaw
The Universitatis Varsoviensis Foundation	Foundation for Polish Science

Warsaw University Foundation	Department of Molecular Biology, International Institute of Molecular and Cell Biology
Units belonging to the Faculty of Biology, University of Warsaw, who kindly provided access to laboratory space and equipment:
Department of Plant Molecular Biology	Department of Virology
Department of Bacterial Genetics	Institute of Genetics and Biotechnology

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 <html>
-<br/>
-<br/>
+<h1>A novel system for generation and screening for new interactors targeted against any bait</h1>
-<div align="center"><img src="https://static.igem.org/mediawiki/2008/6/61/IGEMUWteam-small.gif"/></div>
+<p><ul>
-<p>We want to note that our initial plans were very different from what we actually managed to do. Our plans were really amazing: we wanted to create system of biological machines which would allow to investigate protein interactions and simultaneously change sequence of one of them in order to achieve the strongest possible interaction. To make it work we planned to put the DNA sequence of tested protein on low-copy plasmid and transform it to bacterial strain in which it would be mutated (lets call the strain ‘slot machine’). Parts present on this plasmid would cause the protein to be attached to outer bacterial membrane, where the selection would occur. We planned that such selection system would allow us to search for antibodies with new specificities or screen protein libraries. </p></td>
+<li>maximization of interaction between given bait and randomly generated protein by mutating sequence of the latter</li><li>
-   <td><img src="https://static.igem.org/mediawiki/2008/5/52/Spacer.gif" width="1" height="530" border="0" alt="" /></td>
+interactor presentation on bacterial surface</li><li>
+selection of best bait interactor</li>
+</ul></p>
+<br>
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    </tr>
 <tr><td>
-<h2>Institutions whom we would like to help for financial and technical support:</h2>
+<h3>Institutions whom we would like to thank for financial and technical support:</h3>
 <table width="100%" border="0" cellspacing="10" cellpadding="0" align="center">
    <tr>
      <td align="center" valign="bottom"><a href="http://www.uw.edu.pl/en/"><img src="https://static.igem.org/mediawiki/2008/6/67/Uw.gif" width="100" height="99" border="0" /></a>
-     <a href="http://www.uw.edu.pl/en/"><h3>University of Warsaw</h3> </a></td>
+     <a href="http://www.uw.edu.pl/en/"><h4>University of Warsaw</h4> </a></td>
-     <td align="center" valign="bottom"><a href="http://www.fundacjauv.org.pl/"><img src="https://static.igem.org/mediawiki/2008/4/4d/Fundacja_uv-1.gif" width="160" height="65" border="0" /></a>
+     <td align="center" valign="bottom">
-     <a href="http://www.fundacjauv.org.pl/"><h3>The Universitatis Varsoviensis Fund </h3></a></td>
+<a href="http://www.biol.uw.edu.pl/_2008eng/"><img src="https://static.igem.org/mediawiki/2008/f/f3/Faculty-of-Biology.gif" width="57" height="75" border="0" /></a>
+     <a href="http://www.biol.uw.edu.pl/_2008eng/"><h4>Faculty of Biology, University of Warsaw </h4></a>
+</td>
    </tr>
    <tr>
-     <td align="center" valign="bottom"><a href="http://www.biol.uw.edu.pl/"><img src="https://static.igem.org/mediawiki/2008/f/f3/Faculty-of-Biology.gif" width="91" height="120" border="0" /></a>
+     <td align="center" valign="bottom"><a href="http://www.fundacjauv.org.pl/"><img src="https://static.igem.org/mediawiki/2008/4/4d/Fundacja_uv-1.gif" width="160" height="65" border="0" /></a>
-     <a href="http://www.biol.uw.edu.pl/"><h3>Faculty of Biology, University of Warsaw </h3></a>      </td>
+     <a href="http://www.fundacjauv.org.pl/"><h4>The Universitatis Varsoviensis Foundation </h4></a></td>
-     <td align="center" valign="bottom"><a href="http://www.fnp.org.pl/ang/index.html"><img src="https://static.igem.org/mediawiki/2008/c/c9/LogoFNP1wers.gif" width="312" height="106" border="0" /></a>
+     <td align="center" valign="bottom"><a href="http://www.fnp.org.pl/ang/index.html"><img src="https://static.igem.org/mediawiki/2008/c/c9/LogoFNP1wers.gif" width="221" height="75" border="0" /></a>
-     <a href="http://www.fnp.org.pl/ang/index.html"><h3>Foundation for Polish Science </h3></a></td>
+     <a href="http://www.fnp.org.pl/ang/index.html"><h4>Foundation for Polish Science </h4></a></td>
    </tr>
    <tr>
      <td align="center" valign="bottom"><a href="http://oligo.pl/English/"><img src="https://static.igem.org/mediawiki/2008/8/8e/Oligo.gif" width="183" height="64" border="0" /></a>    </td>
-     <td align="center" valign="bottom"><h3>Rada Konsultacyjna ds. Studenckiego Ruchu Naukowego</h3></td>
+     <td align="center" valign="bottom"><img src="https://static.igem.org/mediawiki/2008/6/60/Rada_Konsultacyjna.gif" width="160" height="120" border="0" /></td>
+  </tr>
+  <tr>
+    <td align="center" valign="bottom">
+<a href="http://www.fuw.pl"><h4>Warsaw University Foundation</h4></a>
+</td>
+    <td align="center" valign="bottom">
+<a href="http://www.iimcb.gov.pl/zylicz_lab.php"><h4>Department of Molecular Biology, International Institute of Molecular and Cell Biology</h4></a>
+</td>
    </tr>
+<tr>
+<td colspan="2" align="center" valign="bottom">
+<p>Units belonging to the <a href="http://www.biol.uw.edu.pl/_2008eng/">Faculty of Biology, University of Warsaw</a>, who kindly provided access to laboratory space and equipment:</p>
+</td>
    <tr>
-     <td align="center" valign="bottom"><a href="http://www.biol.uw.edu.pl/_2008eng/index_2501.php?a_2501=529&amp;b_2501=5"><h3>Department of Bacterial Genetics </h3></a></td>
+     <td align="center" valign="bottom"><a href="http://www.biol.uw.edu.pl/_2008eng/index_2501.php?a_2501=541&amp;b_2501=5"><h4>Department of Plant Molecular Biology </h4></a></td>
-     <td align="center" valign="bottom"><a href="http://www.biol.uw.edu.pl/_2008eng/index_2501.php?a_2501=526&amp;b_2501=5"><h3>Department of Virology</h3> </a></td>
+     <td align="center" valign="bottom"><a href="http://www.biol.uw.edu.pl/_2008eng/index_2501.php?a_2501=526&amp;b_2501=5"><h4>Department of Virology</h4> </a> </td>
    </tr>
    <tr>
-    <td align="center" valign="bottom"><a href="http://www.biol.uw.edu.pl/_2008eng/index_2501.php?a_2501=541&amp;b_2501=5"><h3>Department of Plant Molecular Biology </h3></a></td>
+     <td align="center" valign="bottom">
-    <td align="center" valign="bottom"><a href="http://www.biol.uw.edu.pl/_2008eng/index_2501.php?a_2501=534&amp;b_2501=5"><h3>Institute of Genetics and Biotechnology</h3></a> </td>
+<a href="http://www.biol.uw.edu.pl/_2008eng/index_2501.php?a_2501=529&amp;b_2501=5"><h4>Department of Bacterial Genetics </h4></a>
+</td>
+<td align="center" valign="bottom"><a href="http://www.biol.uw.edu.pl/_2008eng/index_2501.php?a_2501=534&amp;b_2501=5"><h4>Institute of Genetics and Biotechnology</h4></a></td>
    </tr>
 </table>