Imperial College/10 September 2008
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=='''Wet Lab'''== | =='''Wet Lab'''== | ||
===Transformation=== | ===Transformation=== | ||
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===Growth Curve=== | ===Growth Curve=== | ||
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[[Media:OD 600 v Time.xls]] | [[Media:OD 600 v Time.xls]] | ||
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+ | ===Cloning=== | ||
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+ | *All ligations from yesterday failed, no indication of why. 2 test ligations set up and used to transfom Xl1-blue. *Overnight ligations set up for transformation tomorrow | ||
+ | *Constructs 3, 6, 10 and 12 midiprepped | ||
=='''Dry Lab'''== | =='''Dry Lab'''== | ||
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===Growth Curve=== | ===Growth Curve=== | ||
Obtained sigmoidal curve for bacteria growth using MATLAB. | Obtained sigmoidal curve for bacteria growth using MATLAB. | ||
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- | {{Imperial/EndPage}} | + | {{Imperial/EndPage|Notebook|Notebook}} |
Latest revision as of 20:45, 28 October 2008
10 September 2008Wet LabTransformationGrowth CurveWe performed an OD600 against time growth curve for B. subtilis. The results are shown below:
Please click on the link below for the Excel data file:
Cloning
Dry LabMotilityTook 20 videos, binned x4 to a frame rate of 27.9fps, more than twice the rate at which previous videos were taken. However, video quality is not as good as before, but cells can still be easily distinguished. Growth CurveObtained sigmoidal curve for bacteria growth using MATLAB.
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