Team:Hawaii/Notebook/2008-06- 6

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(New page: {{Team:Hawaii/Header}} == Things we did today == ''' Grace and Krystle ''' * Made 10X TE buffer * Made BG-11 + 5% LB plates * Transformed PCC6803 w/ pRL1383a [[Initial_PCC6803_Transformat...)
 
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{{Team:Hawaii/Header}}
{{Team:Hawaii/Header}}
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= Things we did today =
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== Wetlab work ==
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===Prepped for BioBrick Plasmid Extraction===
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:<strong> Grace</strong>
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:Made 10X TE Buffer
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== Things we did today ==
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===* Made BG-11 + 5% LB plates===
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''' Grace and Krystle '''
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:<strong> Grace</strong>
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* Made 10X TE buffer
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* Made BG-11 + 5% LB plates
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* Transformed PCC6803 w/ pRL1383a [[Initial_PCC6803_Transformation |(see experiment)]]
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* Practiced pulling plasmids off filter paper
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''' Norman'''
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===Transformed PCC6803 w/ pRL1383a===
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* Checked PCR products for Norman's pha_genes in agarose gel
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:<strong> Grace and Krystle </strong>
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:[https://2008.igem.org/Team:Hawaii/Initial_PCC6803_Transformation (see experiment)]
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== Discussion ==
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===Cell density test for PCC6803===
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:<strong> Grace</strong>
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* If our action is not recorded, we are doomed to either repeat the error, or fail to replicate the success.
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===Checked PCR products for Norman's pha_genes in agarose gel===
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<blockquote>''History is the only laboratory we have in which to test the consequences of thought.'' - Étienne Gilson</blockquote>
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:<strong> Norman</strong>
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===Thaw test for PCC6803===
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:<strong> Norman</strong>
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== Drylab work ==
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===Prepped for BioBrick Plasmid Extraction===
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:<strong> Krystle</strong>
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:Went through parts registry and found GFP parts and devices for test extractoin
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= Discussion =
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Grace will come back on Sunday to check on PCC6803 tranformants.
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= Quote of the Day =
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<blockquote>''You know, you guys are confusing me even more now -- you're wearing the same shirt.'' -- NW, in reference to constantly getting Grace and Krystle mixed up.
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[[Image:DSC00742.JPG|left|thumb|300 px| Krystle & Grace (No, we do not usually dress alike. Today was just a coincidence.) ]]
{{Team:Hawaii/Footer}}
{{Team:Hawaii/Footer}}

Latest revision as of 01:17, 12 June 2008

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Notebook (t) Meetings (t)

Contents

Things we did today

Wetlab work

Prepped for BioBrick Plasmid Extraction

Grace
Made 10X TE Buffer

* Made BG-11 + 5% LB plates

Grace

Transformed PCC6803 w/ pRL1383a

Grace and Krystle
(see experiment)

Cell density test for PCC6803

Grace

Checked PCR products for Norman's pha_genes in agarose gel

Norman

Thaw test for PCC6803

Norman

Drylab work

Prepped for BioBrick Plasmid Extraction

Krystle
Went through parts registry and found GFP parts and devices for test extractoin


Discussion

Grace will come back on Sunday to check on PCC6803 tranformants.

Quote of the Day

You know, you guys are confusing me even more now -- you're wearing the same shirt. -- NW, in reference to constantly getting Grace and Krystle mixed up.
Krystle & Grace (No, we do not usually dress alike. Today was just a coincidence.)


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