Team:Hawaii/Notebook/2008-06- 6

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{{Team:Hawaii/Header}}
{{Team:Hawaii/Header}}
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= Things we did today =
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== Wetlab work ==
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===Prepped for BioBrick Plasmid Extraction===
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:<strong> Grace</strong>
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:Made 10X TE Buffer
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== Things we did today ==
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===* Made BG-11 + 5% LB plates===
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''' Grace and Krystle '''
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:<strong> Grace</strong>
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* Made 10X TE buffer
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* Made BG-11 + 5% LB plates
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* Transformed PCC6803 w/ pRL1383a [[Initial_PCC6803_Transformation|(see experiment)]]
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* Practiced pulling plasmids off filter paper
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* Cell density test for PCC6803
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''' Norman'''
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===Transformed PCC6803 w/ pRL1383a===
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* Checked PCR products for Norman's pha_genes in agarose gel
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:<strong> Grace and Krystle </strong>
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* Thaw test for PCC6803
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:[https://2008.igem.org/Team:Hawaii/Initial_PCC6803_Transformation (see experiment)]
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== Discussion ==
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===Cell density test for PCC6803===
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:<strong> Grace</strong>
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Grace and Krystle will come back on Sunday to check on PCC6803 tranformants.
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===Checked PCR products for Norman's pha_genes in agarose gel===
 +
:<strong> Norman</strong>
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== Quote of the Day ==
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===Thaw test for PCC6803===
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:<strong> Norman</strong>
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"You know, you guys are confusing me even more now -- you're wearing the same shirt." -- NW, in reference to constantly getting Grace and Krystle mixed up.
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== Drylab work ==
 +
===Prepped for BioBrick Plasmid Extraction===
 +
:<strong> Krystle</strong>
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:Went through parts registry and found GFP parts and devices for test extractoin
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= Discussion =
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Grace will come back on Sunday to check on PCC6803 tranformants.
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= Quote of the Day =
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<blockquote>''You know, you guys are confusing me even more now -- you're wearing the same shirt.'' -- NW, in reference to constantly getting Grace and Krystle mixed up.
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[[Image:DSC00742.JPG|left|thumb|300 px| Krystle & Grace (No, we do not usually dress alike. Today was just a coincidence.) ]]
{{Team:Hawaii/Footer}}
{{Team:Hawaii/Footer}}

Latest revision as of 01:17, 12 June 2008

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Notebook (t) Meetings (t)

Contents

Things we did today

Wetlab work

Prepped for BioBrick Plasmid Extraction

Grace
Made 10X TE Buffer

* Made BG-11 + 5% LB plates

Grace

Transformed PCC6803 w/ pRL1383a

Grace and Krystle
(see experiment)

Cell density test for PCC6803

Grace

Checked PCR products for Norman's pha_genes in agarose gel

Norman

Thaw test for PCC6803

Norman

Drylab work

Prepped for BioBrick Plasmid Extraction

Krystle
Went through parts registry and found GFP parts and devices for test extractoin


Discussion

Grace will come back on Sunday to check on PCC6803 tranformants.

Quote of the Day

You know, you guys are confusing me even more now -- you're wearing the same shirt. -- NW, in reference to constantly getting Grace and Krystle mixed up.
Krystle & Grace (No, we do not usually dress alike. Today was just a coincidence.)


[http://manoa.hawaii.edu/ Sponsor_UHM.gif][http://manoa.hawaii.edu/ovcrge/ Sponsor_OVCRGE.gif][http://www.ctahr.hawaii.edu Sponsor_CTAHR.gif]

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