Team:The University of Alberta/4 September 2008

From 2008.igem.org

(Difference between revisions)
(New page: ==Today== *Did NotI digests of ER1+ER2 & ER1/ER2 - gel shows that it didn't work! ==To Do== *ER1 digest w/ Age/Ngo *ER2 digest w/ Age - add antartic phosphates!!! (prevents re-ligation of...)
(Today)
 
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==Today==
==Today==
 +
/Bisphenolics
*Did NotI digests of ER1+ER2 & ER1/ER2 - gel shows that it didn't work!
*Did NotI digests of ER1+ER2 & ER1/ER2 - gel shows that it didn't work!
-
==To Do==
+
/Butanerds
-
*ER1 digest w/ Age/Ngo
+
*Sequenced 22+24 w/ VF and VR col 1,3,9
-
*ER2 digest w/ Age - add antartic phosphates!!! (prevents re-ligation of vector)
+
*Colony PCR on 21+22+23 and 24+25
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*ER1, ER2, LacIQERE digest w/ Xbal/Pst
+
*Prepped overnights for 22+24 colonies 1,3,4
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*LacTetR, ER1, ER2, into PsBIA3 for submission!!!
+

Latest revision as of 03:10, 17 September 2008

Today

/Bisphenolics

  • Did NotI digests of ER1+ER2 & ER1/ER2 - gel shows that it didn't work!

/Butanerds

  • Sequenced 22+24 w/ VF and VR col 1,3,9
  • Colony PCR on 21+22+23 and 24+25
  • Prepped overnights for 22+24 colonies 1,3,4