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Fluorescence microscopy
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Fluorescence microscopy is a common technique for examining fluorophores (fluorescent molecules). In this case, it was used to show the expression level of two reporter genes, ''gfp'' and ''mcherry''. A light beam from a mecury vapour lamp is focused onto a dichroic mirror, which reflects a adjustable wavelength range onto the sample. This should be the optimum excition wavelength for the fluorophore being observed. In the case of GFP, this is blue light (~395nm). For mCherry, this is yellow light (~590nm). This 'excitation' light causes the fluorophore to emit its own wavelength (~510nm for GFP and ~615nm for mCherry) which is focused onto the camera. In this way, cells (or specific parts of cells) can be visualised depending on where the reporter protein is localised. | Fluorescence microscopy is a common technique for examining fluorophores (fluorescent molecules). In this case, it was used to show the expression level of two reporter genes, ''gfp'' and ''mcherry''. A light beam from a mecury vapour lamp is focused onto a dichroic mirror, which reflects a adjustable wavelength range onto the sample. This should be the optimum excition wavelength for the fluorophore being observed. In the case of GFP, this is blue light (~395nm). For mCherry, this is yellow light (~590nm). This 'excitation' light causes the fluorophore to emit its own wavelength (~510nm for GFP and ~615nm for mCherry) which is focused onto the camera. In this way, cells (or specific parts of cells) can be visualised depending on where the reporter protein is localised. | ||
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Latest revision as of 14:10, 19 September 2008
Fluorescence microscopy is a common technique for examining fluorophores (fluorescent molecules). In this case, it was used to show the expression level of two reporter genes, gfp and mcherry. A light beam from a mecury vapour lamp is focused onto a dichroic mirror, which reflects a adjustable wavelength range onto the sample. This should be the optimum excition wavelength for the fluorophore being observed. In the case of GFP, this is blue light (~395nm). For mCherry, this is yellow light (~590nm). This 'excitation' light causes the fluorophore to emit its own wavelength (~510nm for GFP and ~615nm for mCherry) which is focused onto the camera. In this way, cells (or specific parts of cells) can be visualised depending on where the reporter protein is localised.
Back to Team:Newcastle University/Results
