Team:UNIPV-Pavia/Protocols
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<h1>The protocols we used</h1> | <h1>The protocols we used</h1> | ||
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- | *Plasmid | + | *[[Team:UNIPV-Pavia/Protocols/Lb|LB medium preparation]] |
- | *Transformation | + | *[[Team:UNIPV-Pavia/Protocols/Resuspension|Plasmid resuspension from IGEM paper spots]] |
- | *Plasmid extraction | + | *[[Team:UNIPV-Pavia/Protocols/Transformation|Transformation]] |
- | *BioBrick digestion with restriction enzymes | + | *[[Team:UNIPV-Pavia/Protocols/PlasmidExtraction|Plasmid extraction]] |
- | *DNA gel extraction | + | *[[Team:UNIPV-Pavia/Protocols/Digestion|BioBrick digestion with restriction enzymes]] |
- | * | + | *[[Team:UNIPV-Pavia/Protocols/GelExtraction|DNA gel extraction]] |
- | *Ligation | + | *[[Team:UNIPV-Pavia/Protocols/Precipitation|DNA precipitation with sodium acetate]] |
+ | *[[Team:UNIPV-Pavia/Protocols/AntarcticPhosphatase|Antarctic Phosphatase]] | ||
+ | *[[Team:UNIPV-Pavia/Protocols/Ligation|Ligation]] | ||
+ | *[[Team:UNIPV-Pavia/Protocols/Pcr|PCR]] | ||
<br> | <br> |
Latest revision as of 12:51, 1 July 2008
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The protocols we used
- LB medium preparation
- Plasmid resuspension from IGEM paper spots
- Transformation
- Plasmid extraction
- BioBrick digestion with restriction enzymes
- DNA gel extraction
- DNA precipitation with sodium acetate
- Antarctic Phosphatase
- Ligation
- PCR