Latest revision as of 12:51, 1 July 2008

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 <h1>The protocols we used</h1>
-This page will be updated soon with some links.
-*[[Team:UNIPV-Pavia/Protocols/plasmid_resusp|Plasmid resuspention from IGEM paper spots]]
-*Transformation
-*Plasmid extraction
-*BioBrick digestion with restriction enzymes
-*DNA gel extraction
-*Antartic Phosphatase
-*Ligation
-<br>
+*[[Team:UNIPV-Pavia/Protocols/Lb|LB medium preparation]]
+*[[Team:UNIPV-Pavia/Protocols/Resuspension|Plasmid resuspension from IGEM paper spots]]
+*[[Team:UNIPV-Pavia/Protocols/Transformation|Transformation]]
+*[[Team:UNIPV-Pavia/Protocols/PlasmidExtraction|Plasmid extraction]]
+*[[Team:UNIPV-Pavia/Protocols/Digestion|BioBrick digestion with restriction enzymes]]
+*[[Team:UNIPV-Pavia/Protocols/GelExtraction|DNA gel extraction]]
+*[[Team:UNIPV-Pavia/Protocols/Precipitation|DNA precipitation with sodium acetate]]
+*[[Team:UNIPV-Pavia/Protocols/AntarcticPhosphatase|Antarctic Phosphatase]]
+*[[Team:UNIPV-Pavia/Protocols/Ligation|Ligation]]
+*[[Team:UNIPV-Pavia/Protocols/Pcr|PCR]]
-{|align="center" cellpadding="30"
-!|[[Team:UNIPV-Pavia/Notebook/Week1|Week 1]]
-!|[[Team:UNIPV-Pavia/Notebook/Week2|Week 2]]
-!|[[Team:UNIPV-Pavia/Notebook/Week3|Week 3]]
-!|[[Team:UNIPV-Pavia/Notebook/Week4|Week 4]]
-!|[[Team:UNIPV-Pavia/Notebook/Week5|Week 5]]
-!|[[Team:UNIPV-Pavia/Notebook/Week6|Week 6]]
-!|[[Team:UNIPV-Pavia/Notebook/Week7|Week 7]]
-|}
-<br><br>
-==Week 3: 06/03/08 - 06/08/08==
-'''06/03/08'''
 <br>
-*We transformed 60 µl of TOP10 with 2 µl of the 6 parts (DNA + glycerol) we received from IGEM HQ:
-{|cellpadding="20"
-|BBa_C0179
-|BBa_C0161