Imperial College/19 September 2008
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=19 September 2008= | =19 September 2008= | ||
==Wet Lab== | ==Wet Lab== | ||
- | * | + | *A variety of ligations were carried out last week and to test there success we carried out single colony PCR using Psb primers to verify if an insert is present in the vector AK3. For each ligation thress seperate colonies were picked to carry out SCP and a negative colony where no DNA was present. The gel below shows the resuls of this experiment: |
- | + | [[Image:SCP-19.png|center|500px]] | |
+ | *The conditions used were as follows: | ||
+ | **1 cycle - 95<sup>o</sup>C for 30 seconds | ||
+ | **30 cycles - 95<sup>o</sup>C for 30 seconds, 60<sup>o</sup>C for 30 seconds,72<sup>o</sup>C for 30 seconds | ||
+ | **1 cycle - 72<sup>o</sup>C for 2 minutes | ||
+ | *The numbers of the ligations correspond to the following ligation reactions: | ||
+ | **5-Eps+AK3 | ||
+ | **8-PgsiB-gsiB+AK3 | ||
+ | **9-PgsiB-spoVG+AK3 | ||
+ | **12-Pveg-spoVG | ||
==Dry Lab== | ==Dry Lab== | ||
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[[Image:Velocity_Distribution_90_Cells.jpg|thumb|center|300px|Run velocity distribution for 90 cells]] | [[Image:Velocity_Distribution_90_Cells.jpg|thumb|center|300px|Run velocity distribution for 90 cells]] | ||
+ | <br> | ||
+ | {{Imperial/EndPage|Notebook|Notebook}} |
Latest revision as of 20:48, 28 October 2008
19 September 2008Wet Lab
Dry Lab
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