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Illinois/31 July 2008
From 2008.igem.org
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(New page: '''Receptor Tyrosie Kinase Group''' PCR to asemble fragments from yesterday. {| class="wikitable" border="1" |- |tube |1 |2 |3 |4 |- |template |colspan="4"|0.5ul of both heavy and light ...) |
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Latest revision as of 04:11, 23 September 2008
Receptor Tyrosie Kinase Group
PCR to asemble fragments from yesterday.
| tube | 1 | 2 | 3 | 4 | ||||
| template from 7-30 PCR | 2+3 | 2+7 | 6+7 | 1+8 | ||||
| template | 0.5ul of both heavy and light chains | |||||||
| primers | 1ul of appropriate forward and reverse primer | |||||||
| MgCl2 | 0ul | 0ul | 3ul | 3ul | ||||
| master mix | 20ul | |||||||
| H20 | to 50ul total volume | |||||||
PCR program: 1. 94 degrees 5 min 2. 94 degrees 1 min 3. 50 degrees 1 min 4. 72 degrees 1 min 5. GOTO 2. 29 cycles 6. HOLD at 4 degrees
Products run on a 1.5% agarose gel, no products of 700-800bp.
