Team:Warsaw/Calendar-Main/9 June 2008

From 2008.igem.org

(Difference between revisions)
 
(15 intermediate revisions not shown)
Line 2: Line 2:
<!-- do not edit above me! -->
<!-- do not edit above me! -->
 +
<html><h3>Preparation of <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pMPM-T5-AID%2BAID-T7>pMPMT5-AID+AIDT7</a> construct</h3>
 +
<h4>Michał K.</h4>
 +
<ol>
 +
<li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#digest">Digest</a> of confirmed plasmid <A href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pMPM-T5-AID%2BAID-T7>pMPMT5-AID+AID-T7</a> with HindIII and SalI (2x Tango buffer).</li>
 +
<li>DNA gel electrophoresis.</li>
 +
<li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#DNA_isolation_from_agarose_gel">Isolation of DNA</a> from proper band (3300 bp).</li>
 +
<li> Sequencing of isolated band with <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#AIDseqP">AIDseqP</a> and <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#T7Rseq">T7Rseq</a>. </li></ol>
-
 
+
</html>
-
<p> Inoculation of 5 colonies of transformants (pMPM-T5 + AID transcription fusion with AID-T7 RNA-polymerase translation fusion) into liquid LB broth.<br>
+
-
 
+
-
Inoculation of 5 colonies of transformants (pMPM-T5 + T7 RNA-polymerase) into liquid LB broth</p>
+
-
 
+
-
 
+
-
 
+
-
 
+
<!-- do not remove this! -->
<!-- do not remove this! -->
{{WarNotebookEnd}}
{{WarNotebookEnd}}

Latest revision as of 15:15, 24 October 2008

Gallery Bricks Notebook Team Project Home


Previous day
return to main notebook page
Previous entry
Interactive list of topics
next notebook entry

 


Preparation of pMPMT5-AID+AIDT7 construct

Michał K.

  1. Digest of confirmed plasmid pMPMT5-AID+AID-T7 with HindIII and SalI (2x Tango buffer).
  2. DNA gel electrophoresis.
  3. Isolation of DNA from proper band (3300 bp).
  4. Sequencing of isolated band with AIDseqP and T7Rseq.

Retrieved from "http://2008.igem.org/Team:Warsaw/Calendar-Main/9_June_2008"